Trpm3 (NM_001191562) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217685 representing NM_001191562 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAAGAAGTGGAGGGACGCGGGCGAGATGGAGCGAGGCTGCTCTGATCGCGAGGGCAGCGCGGAGA GCCGCCGGCGCAGCCGCAGCGCCAGCCGGGGCAGGTTTGCCGAGTCGTGGAAAAGGTTAAGTTCCAAGCA GGGGTCCACCAAGCGCTCGGGACTGCCAGCACAGCAGACACCGGCTCAGAAATCCTGGATTGAAAGAGCA TTTTATAAAAGAGAATGTGTTCACATCATACCCAGCACCAAAGACCCCCATAGGTGTTGCTGTGGGCGCC TGATAGGCCAGCATGTCGGACTCACTCCCAGTATCTCTGTGCTTCAGAATGAGAAAAATGAGAGTCGCCT CTCCCGAAATGACATCCAGTCTGAGAAGTGGTCTATCAGCAAACACACTCAGCTCAGCCCAACGGATGCT TTTGGGACTATTGAGTTCCAAGGAGGCGGTCATTCCAACAAAGCCATGTATGTCCGAGTATCTTTTGATA CGAAACCTGATCTCTTGCTACACCTGATGACCAAGGAATGGCAACTAGAGCTTCCCAAACTTCTCATCTC TGTGCACGGAGGGCTGCAGAACTTTGAACTCCAGCCCAAACTCAAGCAAGTCTTCGGGAAAGGGCTCATC AAAGCAGCCATGACAACTGGAGCCTGGATCTTCACTGGAGGGGTCAACACAGGTGTCATCCGTCATGTGG GAGATGCCTTGAAGGACCACGCATCTAAGTCTCGAGGGAAGATATGCACCATAGGTATCGCACCCTGGGG AATTGTGGAAAACCAGGAAGACCTGATCGGAAGAGATGTAGTCCGACCATACCAGACCATGTCAAACCCT ATGAGCAAGCTCACGGTTCTCAACAGCATGCACTCCCACTTCATCCTGGCTGACAATGGGACCACGGGAA AATACGGGGCCGAGGTGAAACTCCGCAGACAACTGGAAAAGCACATTTCACTCCAGAAGATAAACACAAG AATCGGTCAAGGGGTTCCAGTGGTGGCCCTCATCGTGGAAGGAGGACCCAATGTGATCTCAATTGTTTTG GAATACCTTCGAGACACCCCTCCTGTACCAGTCGTGGTCTGCGATGGCAGTGGACGGGCATCCGACATTC TGGCATTTGGGCATAAATATTCAGAAGAAGGCGGTCTTATCAATGAATCTTTGAGGGACCAGCTGTTGGT GACAATACAGAAGACCTTCACATACACCCGCACCCAAGCCCAGCACCTGTTCATCATCCTCATGGAATGC ATGAAGAAGAAGGAATTGATCACAGTGTTTCGAATGGGATCAGAAGGCCACCAGGACATTGATTTAGCTA TCCTGACAGCACTACTCAAAGGAGCCAATGCATCCGCCCCAGACCAGCTGAGCTTAGCATTAGCCTGGAA CAGAGTTGACATCGCTCGCAGCCAGATCTTTATTTACGGGCAGCAGTGGCCGGTAGGGTCCCTGGAGCAA GCCATGCTGGATGCCCTAGTCCTGGACAGAGTGGACTTTGTGAAATTACTCATAGAAAACGGAGTAAGCA TGCACCGTTTTCTCACCATCTCCAGACTAGAGGAACTGTACAATACGAGACATGGGCCCTCAAATACATT GTACCACTTGGTCAGGGATGTCAAAAAGGGAAACCTGCCCCCGGACTACAGAATCAGCCTGATAGACATT GGCCTGGTGATCGAGTACCTGATGGGTGGGGCTTACCGCTGCAACTACACACGCAAGCGCTTTCGGACCC TCTACCACAATCTCTTTGGCCCCAAAAGGCCCAAAGCCTTGAAACTGCTGGGAATGGAGGATGATATTCC CTTGAGGAGAGGAAGAAAGACAACTAAGAAACGTGAGGAAGAGGTGGACATAGACTTGGATGACCCTGAG ATCAACCACTTCCCATTCCCTTTCCATGAGCTGATGGTGTGGGCTGTCCTCATGAAGAGGCAGAAGATGG CCCTGTTCTTCTGGCAGCATGGAGAGGAGGCCATGGCTAAAGCCCTGGTGGCCTGCAAGCTCTGCAAAGC CATGGCCCATGAGGCTTCTGAGAATGATATGGTGGATGACATTTCCCAAGAGCTGAACCACAACTCCAGG GACTTTGGCCAGCTGGCTGTGGAGCTTTTGGACCAGTCCTACAAACAGGATGAGCAGCTGGCCATGAAAC TGCTGACGTATGAGTTGAAGAACTGGAGCAATGCCACATGCCTGCAGCTGGCTGTGGCTGCCAAGCACCG TGACTTCATTGCACACACGTGCAGCCAGATGTTACTTACCGACATGTGGATGGGACGGCTCCGCATGCGA AAGAACTCAGGCCTCAAGGTAATTCTGGGAATTCTACTTCCTCCTTCAATTCTCAGCTTGGAGTTCAAGA ACAAAGATGACATGCCCTATATGACTCAGGCCCAAGAAATTCATCTCCAAGAGAAGGAGCCGGAGGAACC GGAGAAGCCTACAAAGGAAAAAGATGAAGAGGACATGGAGCTAACAGCAATGTTGGGACGAAACAATGGG GAGTCATCCAGAAAGAAAGACGAAGAAGAAGTTCAAAGCAGGCATCGACTAATCCCTGTGGGCCGAAAAA TCTATGAGTTCTACAACGCACCCATTGTGAAGTTCTGGTTCTACACTCTGGCGTACATTGGATACCTGAT GCTCTTCAATTACATCGTGCTGGTGAAGATGGAGCGCTGGCCTTCCACTCAGGAATGGATCGTCATCTCC TACATCTTCACTCTGGGAATAGAGAAGATGAGAGAGATCCTGATGTCAGAGCCGGGCAAGCTGCTGCAGA AAGTCAAAGTGTGGCTGCAGGAGTACTGGAACGTCACGGACCTCATCGCCATCCTCCTCTTCTCGGTGGG AATGATCCTTCGTCTTCAAGACCAGCCCTTCAGGAGTGACGGGAGGGTCATCTACTGTGTGAACATCATT TATTGGTATATCCGTTTACTAGACATCTTCGGCGTGAACAAGTATCTGGGCCCATACGTAATGATGATTG GGAAAATGATGATAGACATGATGTACTTCGTCATCATTATGCTGGTGGTGCTGATGAGCTTTGGGGTCGC CAGGCAAGCCATTCTCTTTCCCAATGAGGAGCCATCTTGGAAACTGGCCAAGAATATCTTCTACATGCCT TATTGGATGATTTATGGGGAAGTGTTTGCTGACCAGATAGACCGTAAGCAAGTTTATGATTCTCATACAC CAAAGTCAGCTCCCTGTGGACAGAACGAGACCCGAGAGGATGGCAAGACAATCCAGCTGCCCCCATGCAA GACAGGAGCATGGATTGTGCCAGCCATCATGGCCTGCTACCTCTTAGTAGCGAACATCCTTCTGGTCAAC CTCCTTATTGCTGTCTTCAACAATACATTTTTTGAGGTCAAGTCGATATCCAACCAAGTATGGAAATTTC AGAGGTACCAGCTCATCATGACTTTCCATGAGAGACCAGTTCTGCCTCCACCGCTCATCATCTTCAGTCA CATGACCATGATATTCCAGCATGTGTGCTGCCGATGGAGGAAGCATGAGAGTGACCCGGATGAAAGGGAC TACGGCCTGAAACTCTTCATAACTGATGATGAGCTCAAGAAAGTACATGATTTTGAAGAGCAGTGCATAG AGGAATATTTCAGAGAGAAGGATGACCGCTTCAATTCATCCAATGATGAGAGGATACGGGTTACGTCAGA AAGGGTGGAGAACATGTCCATGAGGCTGGAGGAAGTCAATGAGAGAGAACATTCCATGAAGGCTTCACTC CAGACTGTGGACATCCGGCTAGCACAGCTGGAGGATCTCATCGGGCGCATGGCCACTGCCCTGGAGCGCC TAACAGGTCTGGAGCGGGCAGAGTCTAACAAAATCCGCTCAAGGACCTCCTCAGACTGCACAGACGCAGC CTACATCGTCCGCCAGAGCAGCTTCAACAGTCAGGAAGGGAACACCTTCAAACTTCAAGAGAGTATAGAC CCTGCAGGTGAGGAGACCATGTCCCCAACTTCTCCAACCTTAATGCCCCGTATGCGAAGCCATTCTTTCT ATTCGGTCAATGTGAAAGACAAAGGTGGTATAGAAAAGTTGGAGAGCATTTTCAAAGAAAGGTCCCTGAG CTTACACCGTGCTACTAGCTCCCACTCAGTAGCCAAGGAGCCCAAAGCTCCTGCAGCCCCTGCAAACACC TTGGCCATTGTTCCTGATTCCAGAAGACCATCGTCATGCATAGACATCTATGTCTCTGCCATGGATGAGC TCCACTGTGATATAGACCCTCTGGATAATTCCATGAACATCCTTGGGCTGGGCGAGCCAAGCTTTTCAGC TCTAGCACCTTCCACAGCCCCTTCAAGCAGTGCCTATGCAACCCTTGCACCTACAGACCGACCTCCAAGC AGGAGCATTGATTTTGAAGACCTCACCTCCATGGACACTAGATCTTTTTCTTCAGACTATACCCACCTCC CAGAATGCCAAAACCCCTGGGACACAGACCCTCCAATGTACCATACCATCGAGCGTTCCAAGAGTAGCCG CTACCTAGCCACTACACCCTTCCTTCTGGAAGAGGCCCCCATTGTAAAATCCCATAGCTTTATGTTTTCT CCTTCAAGGAGTTACTACGCCAACTTTGGGGTGCCCGTGAAAACAGCAGAATACACAAGTATTACAGACT GTATCGACACAAGATGTGTCAATGCCCCCCAAGCAATAGCTGACAGAGCCACCTTCCCCGGAGGTCTCGG AGACAAAGTGGAAGATTTATCTTGTTGCCACCCTGAGCGAGAAGCAGAGCTGAGCCACCCTAGCTCTGAC AGTGAGGAAAATGAGGCCAGAGGCCGGAGAGCTGCCAATCCGATATCCTCTCAGGAGACTGAAAATGCAG ACAGAACCCTATCCAACAACATCACAGTTCCCAAGATAGAGCGCGCCAACAGCTACTCGGCAGAGGAGCC AAGCGCGCCATATGCACATACCAGAAAGAGCTTCTCCATCAGTGACAAGCTTGATAGACAGAGGAACACA GCAAGCCTCCGAAATCCCTTCCAGAGAAGTAAGTCCTCCAAGCCGGAGGGCCGAGGGGACAGCGTATCCA TGAGAAGACTGTCTAGAACATCGGCTTTTCATAGCTTTGAGAGCAAGCACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217685 representing NM_001191562 Red=Cloning site Green=Tags(s) MGKKWRDAGEMERGCSDREGSAESRRRSRSASRGRFAESWKRLSSKQGSTKRSGLPAQQTPAQKSWIERA FYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTPSISVLQNEKNESRLSRNDIQSEKWSISKHTQLSPTDA FGTIEFQGGGHSNKAMYVRVSFDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLI KAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLIGRDVVRPYQTMSNP MSKLTVLNSMHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVL EYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGLINESLRDQLLVTIQKTFTYTRTQAQHLFIILMEC MKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQ AMLDALVLDRVDFVKLLIENGVSMHRFLTISRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDI GLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDIPLRRGRKTTKKREEEVDIDLDDPE INHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNHNSR DFGQLAVELLDQSYKQDEQLAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMR KNSGLKVILGILLPPSILSLEFKNKDDMPYMTQAQEIHLQEKEPEEPEKPTKEKDEEDMELTAMLGRNNG ESSRKKDEEEVQSRHRLIPVGRKIYEFYNAPIVKFWFYTLAYIGYLMLFNYIVLVKMERWPSTQEWIVIS YIFTLGIEKMREILMSEPGKLLQKVKVWLQEYWNVTDLIAILLFSVGMILRLQDQPFRSDGRVIYCVNII YWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILFPNEEPSWKLAKNIFYMP YWMIYGEVFADQIDRKQVYDSHTPKSAPCGQNETREDGKTIQLPPCKTGAWIVPAIMACYLLVANILLVN LLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHMTMIFQHVCCRWRKHESDPDERD YGLKLFITDDELKKVHDFEEQCIEEYFREKDDRFNSSNDERIRVTSERVENMSMRLEEVNEREHSMKASL QTVDIRLAQLEDLIGRMATALERLTGLERAESNKIRSRTSSDCTDAAYIVRQSSFNSQEGNTFKLQESID PAGEETMSPTSPTLMPRMRSHSFYSVNVKDKGGIEKLESIFKERSLSLHRATSSHSVAKEPKAPAAPANT LAIVPDSRRPSSCIDIYVSAMDELHCDIDPLDNSMNILGLGEPSFSALAPSTAPSSSAYATLAPTDRPPS RSIDFEDLTSMDTRSFSSDYTHLPECQNPWDTDPPMYHTIERSKSSRYLATTPFLLEEAPIVKSHSFMFS PSRSYYANFGVPVKTAEYTSITDCIDTRCVNAPQAIADRATFPGGLGDKVEDLSCCHPEREAELSHPSSD SEENEARGRRAANPISSQETENADRTLSNNITVPKIERANSYSAEEPSAPYAHTRKSFSISDKLDRQRNT ASLRNPFQRSKSSKPEGRGDSVSMRRLSRTSAFHSFESKHN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191562
ORF Size	5163 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191562.1, NP_001178491.1
RefSeq Size	6740 bp
RefSeq ORF	5166 bp
Locus ID	309407
MW	196.1 kDa

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