Lamc2 (NM_001100640) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217538 representing NM_001100640 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCGCTCTGGCTCAGCTGCTGCCTCGGGGTCGCGCTCCTGCTGCCAGCAGCCCAGGCCACCTCCA GGAGAGAAGTCTGTGATTGCAATGGGAAGTCCAGGCAGTGCGTCTTTGACCAGGAGCTCCATCGACAGAC AGGCAATGGGTTCCGTTGCCTCAACTGCAATGACAATACGGCCGGGGTTCACTGCGAGAGGTGCAGGGAG GGGTTTTACCGGCAGCGAGACAGAGATCGCTGCCTGCCCTGCAACTGCCACTCAAAAGGTTCCCTCGGTG CTGGATGTGACAACTCTGGACAATGCAGGTGTAAGCCAGGTGTGGTAGGACAGAGGTGTGACCGATGTCA GCCAGGCTTCCATATGCTCACCGATGCTGGATGTACCCGAGACCAGAGGCAACTAGATTCCAAGTGTGAC TGTGACCCAGCCGGCATCTCTGGACCCTGTGATTCTGGCCGATGTGTGTGCAAACCAGCCGTCACTGGAG AACGCTGTGACAGGTGCCGAGCAGGCTACTATCATCTGGACAGGGCAAACCCTGAGGGCTGCACCCAGTG TTTCTGCTATGGGCATTCAGACAGCTGCCACACCTCTGCTGACTTCAGTGTCCACAAAATCACCTCAACT TTCAATCAGGATGAAGATGGTTGGAAAGCAGTTCAGAGAAATTGGGCGCCTGCAAAACTCCACTGGTCAC CGCGCCATCAGGATGTATTTAGTTCTGCCCGAAGATCAGACCCAGTCTATTTCGTGGCACCCGCCAAATT TCTCGGTAATCAGCAAGTGAGTTACGGGCAGAGCCTGTCTTTTGACTACCGCGTGGACAGAGGAGGTCGA CACCCATCTGCCTATGATGTGATCCTGGAAGGTGCTGGTCTACAGATCAGAGCTCCTCTGATGCCACCAG GCAAGACACTTCCCTGTGGGATCACAAAGACTTACACGTTCAGATTAAACGAACATCCAAGCAGTCACTG GAGCCCCCAGCTGAGTTATTTCGAGTATCGAAGGTTACTGCGGAACCTCACAGCCCTCCTCATCCGAGCT ACGTACGGAGAATACAGTACAGGGTACATTGACAATGTGACCCTGATTTCAGCCCGCCCTGTCTCTGGAG CCCCAGCACCCTGGGTTGAACGTTGTGTATGCCCTGCTGGGTACAAGGGACAATTCTGCCAGGAATGTGC TTCTGGTTACAAAAGAGATTCAGCAAGACTGGGGCCTTTTGGCACCTGTGTTCCCTGTAACTGCCAAGGG GGAGGGGCCTGTGACCCAGACACAGGGGATTGCTACTCAGGGGACGAGAATCCTGACATTGAGTGTGCCG ACTGCCCCATTGGTTTCTATAACGACCCGCACGACCCCCGCAGCTGCAAGCCGTGTCCCTGCCACAATGG CTTCAGCTGTTCCGTGATGCCTGAGACAGAGGAGGTGGTGTGCAACAACTGCCCCCCTGGGGTCACAGGT GCCCGCTGTGAGCTCTGTGCAGATGGCTTCTTTGGGGACCCCTTTGGGGAACGTGGCCCAGTGAGGCCTT GTCAGCGGTGCCAATGCAACAACAATGTGGACCCCAGTGCTTCTGGGAACTGTGACCAGTTGACAGGCAG ATGTTTGAAATGCATCTACAACACAGCGGGAATCTACTGTGACCAGTGCAAAGCAGGTTACTTCGGAGAC CCATTGGCTCCCAACCCAGCAGACAAGTGTCGAGCTTGCAACTGCAACCCTGTGGGCTCGGAGCCTGGAG AGTGTCGAAGTGATGGCAGCTGTGTTTGCAAGCCAGGCTTTGGAGGCCTCAACTGTGCGCACGCCGCCGC CCTAACCAGTTGCCCTGCTTGCTACAATCAAGTGAAGACTCAGATGGACCAGTTTGCCCAGCAGCTCCAG AACTTGGAGGCCCTGGTTTCAAAGGCTCAGGGTGGTAATGGTGCAGTCCCCAGCGAGGAGCTGGAAGGCA GGATGCAGCAGGCTGAGCAGGCCCTTCGAGACATTCTGAGAGAAGCTCAGATTTCAGAAGGTGCTATGAG AGCCCTCAGCCTCCAGCTGGCCAAGGCAAGGAGCCAAGAGAACAACTACAAGAACCGCCTGGATGACCTC AAGATGACTGTGGAAAGGATTCGGGCCCTGGGCAGTCAGTATCAGAACCGAGTTCAGGATACAAGCAGAC TCATCTCTCAGATGCGCCTGAGCCTGGCAGAAAGTGAAGCTTCCCTGCAAAACACTAACATCCATTCCTC TGAGCACTACGTGGGGCCAAATGGATTTAAAAGTCTGGCTCAGGAGGCCGCAAGATTGGCAGATAGCCAT GTTGAGTCAGCTAACACAATGAAGCAGCTAACGAGGGAAACCGAGGACTATTCCAAACAAGCACTTTCAT TGGCCCGCAAGGCTCTGAGTGGAGGAGGTGGAAGCGGTGTCCTGGACAGCTCCGTGGTACAAGGTCTTAT GGGAAAATTAGAGAAAACCAAGTCCCTGACCCAGCAGTTGTCAAGGGAGGGCACCCAAGCCGACATCGAA GCTGATAGGTCCTATCAACATAGTCTCCGCCTCCTGGATTCAGCGTCTCAGCTTCAGGGAATCCGTGATT CCTCTTTTCAGGCAGAGGCAAAGAGGATCAGGCAGAAGGCTGATTCCCTCTCCAACTTGGTGACCAAACA AATGGATGCATTCACGAGTGTGCGAAACAATCTGGGGAACTGGGAAAAAGAAACACGGCAGCTCTTGCAG ACTGGGAAGGACAGAAGACAGACTTCGGATCAGCTGCTTTCCCGTGCCAACCTTGCTAAAAGCAGAGCCC AAGAAGCGCTAAGTATGGGCAATGCCACTTTTTATGAAGTTGAGAACATCCTAAAGAACCTCAGAGAGTT TGATCTGCAGGTTGAAGACAGAAAAGCAGAGGCTGAGGAGGCCATGAAGAGACTCTCCTATATTAGCCAG AAGGTTGCAGATGCCAGTGACAAGACCCAGCAAGCAGAAACGGCCCTGGGGAGCGCCACTGCTGACACCC AACGGGCAAAGAACGCAGCTACGGAGGCCCTAGAGATCACCAGCGAGATAGAGCAGGAGATAGGGAGTCT GAACTTGGAAGCCAATGTGACAGCAGATGGGGCCTTGGCCATGGAAAAGGGACTGGCCACTCTGAAGAGC GAGATGAGAGAGGTGGAAGGGGAGTTGGCCAGGAAGGAGCTTGAGTTCCACACGGATAAAGACTCCTTGC AGCTGGTGATTACTGAAGCCCAGCAAGCTGATGCCAGAGCCAAGAGCACGGGAGTTACGATCCAAGACAC GCTCAACACATTGGAAGGCATCCTGCGCCTAATAGACCAGCCTGATGCTGTGGATGAAGAGGGGCTGATG CTGTTAGAACAAGAGCTTTTCCAAGCCAAGACCCAGATCAACAGTCGACTTCGGCCCTTGATGTCTGAAC TGGAGGACAGGGCGCGTCGACAGAGCAACCACCTCCACCTGCTGGAGACCAGCATAGATGGGATTCTAGC CGATGTGAAGAACCTGGAGAACATTCGAGACAACCTGCCCCCAGGCTGCTACAACACCCAAGCTCTTGAG CAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217538 representing NM_001100640 Red=Cloning site Green=Tags(s) MPALWLSCCLGVALLLPAAQATSRREVCDCNGKSRQCVFDQELHRQTGNGFRCLNCNDNTAGVHCERCRE GFYRQRDRDRCLPCNCHSKGSLGAGCDNSGQCRCKPGVVGQRCDRCQPGFHMLTDAGCTRDQRQLDSKCD CDPAGISGPCDSGRCVCKPAVTGERCDRCRAGYYHLDRANPEGCTQCFCYGHSDSCHTSADFSVHKITST FNQDEDGWKAVQRNWAPAKLHWSPRHQDVFSSARRSDPVYFVAPAKFLGNQQVSYGQSLSFDYRVDRGGR HPSAYDVILEGAGLQIRAPLMPPGKTLPCGITKTYTFRLNEHPSSHWSPQLSYFEYRRLLRNLTALLIRA TYGEYSTGYIDNVTLISARPVSGAPAPWVERCVCPAGYKGQFCQECASGYKRDSARLGPFGTCVPCNCQG GGACDPDTGDCYSGDENPDIECADCPIGFYNDPHDPRSCKPCPCHNGFSCSVMPETEEVVCNNCPPGVTG ARCELCADGFFGDPFGERGPVRPCQRCQCNNNVDPSASGNCDQLTGRCLKCIYNTAGIYCDQCKAGYFGD PLAPNPADKCRACNCNPVGSEPGECRSDGSCVCKPGFGGLNCAHAAALTSCPACYNQVKTQMDQFAQQLQ NLEALVSKAQGGNGAVPSEELEGRMQQAEQALRDILREAQISEGAMRALSLQLAKARSQENNYKNRLDDL KMTVERIRALGSQYQNRVQDTSRLISQMRLSLAESEASLQNTNIHSSEHYVGPNGFKSLAQEAARLADSH VESANTMKQLTRETEDYSKQALSLARKALSGGGGSGVLDSSVVQGLMGKLEKTKSLTQQLSREGTQADIE ADRSYQHSLRLLDSASQLQGIRDSSFQAEAKRIRQKADSLSNLVTKQMDAFTSVRNNLGNWEKETRQLLQ TGKDRRQTSDQLLSRANLAKSRAQEALSMGNATFYEVENILKNLREFDLQVEDRKAEAEEAMKRLSYISQ KVADASDKTQQAETALGSATADTQRAKNAATEALEITSEIEQEIGSLNLEANVTADGALAMEKGLATLKS EMREVEGELARKELEFHTDKDSLQLVITEAQQADARAKSTGVTIQDTLNTLEGILRLIDQPDAVDEEGLM LLEQELFQAKTQINSRLRPLMSELEDRARRQSNHLHLLETSIDGILADVKNLENIRDNLPPGCYNTQALE QQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100640
ORF Size	3576 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100640.1, NP_001094110.1
RefSeq Size	5157 bp
RefSeq ORF	3579 bp
Locus ID	192362
MW	130.9 kDa
Gene Summary	extracellular matrix glycoprotein; plays a major role in cell migration and differentiation [RGD, Feb 2006]

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