United States
Germany
Japan
United Kingdom
China
Agxt (NM_030656) Rat Tagged ORF Clone
CAT＃: RR214253
- TrueORF®
Agxt (Myc-DDK-tagged ORF) - Rat alanine-glyoxylate aminotransferase (Agxt), (10 ug)
"NM_030656" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AGT; Spat; SPT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214253 representing NM_030656
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCGGATGTTGGCCAAGGCCAGTGTGACGCTGGGCTCCAGGGCAGCAAGTTGGGTACGGAACATGG GCTCGCACCAGCTGCTGGTGCCACCCCCAGAGGCCCTGAGCAAGCCCCTGTCAATTCCTAAGAGGCTCCT GTTGGGTCCGGGACCCTCCAACCTGGCTCCTCGTGTGCTAGCAGCTGGAAGTCTGAGGATGATTGGCCAC ATGCAAAAAGAGATGTTTCAGATCATGGATGAGATCAAGCAGGGCATCCAGTATGTGTTCCAGACCAGGA ACCCCCTCACACTGGTTGTCAGCGGCTCAGGACATTGTGCCATGGAGACTGCCCTGTTCAACCTCCTGGA GCCTGGGGACTCCTTTCTTGTGGGAACCAATGGCATCTGGGGGATACGGGCTGCAGAGATCGCTGAGCGG ATTGGAGCCCGTGTGCACCAGATGATCAAGAAGCCTGGAGAACATTACACACTGCAGGAGGTGGAGGAGG GCCTGGCTCAGCATAAACCAGTGTTGCTGTTCCTGACCCACGGGGAGTCATCCACTGGTGTGCTGCAGCC CCTGGATGGTTTCGGGGAGCTCTGCCACAGGTATCAGTGCCTACTCCTGGTGGACTCGGTGGCATCATTG GGCGGAGTCCCTATCTACATGGACCAACAAGGCATCGACATCTTGTACTCTGGCTCTCAGAAGGTCCTGA ATGCCCCACCAGGGATCTCCCTCATCTCCTTCAACGACAAGGCCAAATCCAAAGTCTACTCCCGGAAGAC AAAGCCAGTCTCCTTCTACACAGACATCACTTATTTGTCCAAGTTGTGGGGCTGTGAGGGCAAGACCAGA GTAATTCATCATACGTTGCCTGTCATCAGCTTATACTGCCTGAGGGAGAGCCTAGCACTCATTTCAGAGC AGGGCCTGGAGAATTCCTGGCGGCGTCACAGGGAGGCTACAGCACATCTGCACAAGTGCCTGCGGGAGTT GGGCTTAAAGTTCTTTGTGAAGGACCCGGAAATCCGGCTACCTACAATCACCACCGTGACCGTGCCTGCC GGCTACAACTGGAGGGACATCGTCAGCTACGTGCTGGACCACTTCAACATTGAAATCTCTGGTGGTCTTG GGCCCTCTGAGGATAAGGTGCTGCGGATTGGCCTCCTGGGCTACAACGCCACCACAGAGAATGCGGACCG TGTAGCGGAGGCCCTGAGGGAGGCCCTGCAACATTGTCCTAAGAATAAATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214253 representing NM_030656
Red=Cloning site Green=Tags(s) MFRMLAKASVTLGSRAASWVRNMGSHQLLVPPPEALSKPLSIPKRLLLGPGPSNLAPRVLAAGSLRMIGH MQKEMFQIMDEIKQGIQYVFQTRNPLTLVVSGSGHCAMETALFNLLEPGDSFLVGTNGIWGIRAAEIAER IGARVHQMIKKPGEHYTLQEVEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYQCLLLVDSVASL GGVPIYMDQQGIDILYSGSQKVLNAPPGISLISFNDKAKSKVYSRKTKPVSFYTDITYLSKLWGCEGKTR VIHHTLPVISLYCLRESLALISEQGLENSWRRHREATAHLHKCLRELGLKFFVKDPEIRLPTITTVTVPA GYNWRDIVSYVLDHFNIEISGGLGPSEDKVLRIGLLGYNATTENADRVAEALREALQHCPKNKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030656 |
| ORF Size | 1242 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030656.2, NP_085914.2 |
| RefSeq Size | 1628 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 24792 |
| UniProt ID | P09139 |
| MW | 45.8 kDa |
| Gene Summary | This gene encodes alanine-glyoxylate aminotransferase, which catalyzes the interconversion of L-alanine and glyoxylate to pyruvate and glycine. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. The longer transcript variant includes an upstream translation start codon and a downstream translation start codon. The upstream start codon initiates the translation of the mitochondrial enzyme precursor while the downstream start codon initiates the translation of the peroxisomal enzyme (see PMID:2332438). [provided by RefSeq, Feb 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214253 | Agxt (untagged ORF) - Rat alanine-glyoxylate aminotransferase (Agxt), (10 ug) |
CNY 3,990.00 |
|
| RR214253L3 | Lenti ORF clone of Agxt (Myc-DDK-tagged ORF) - Rat alanine-glyoxylate aminotransferase (Agxt), (10 ug) |
CNY 6,080.00 |
|
| RR214253L4 | Lenti ORF clone of Agxt (mGFP-tagged ORF) - Rat alanine-glyoxylate aminotransferase (Agxt), (10 ug) |
CNY 6,650.00 |
