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Adam17 (NM_020306) Rat Tagged ORF Clone
CAT＃: RR214103
- TrueORF®
Adam17 (Myc-DDK-tagged ORF) - Rat ADAM metallopeptidase domain 17 (Adam17), (10 ug)
"NM_020306" in other vectors (3)
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CNY 10,550.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TACE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214103 representing NM_020306
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCAGCGTCTCCTCTTCCTGACCACTTTGGTGCCTTTCGTCCTGGCACCCCGACCTCCGGAGGAAC CAGGCTCTGGCTCCCACCTGCGACTTGAGAAGCTTGATTCTTTGCTCTCAGACTACGATATCCTCTCTTT ATCTAATATCCAGCAGCACTCCATAAGGAAAAGGGATCTACAGTCTGCGACACACTTAGAAACACTACTA ACTTTTTCAGCTTTGAAAAGGCATTTTAAATTATACTTGACATCAAGTACAGAACGCTTTTCACAGAACT TGCGAGTCGTGGTGGTGGATGGGAAAGAGGAAAGCGAGTACAGTGTGAAGTGGCAGGACTTCTTCAGCGG ACATGTGGTTGGTGAGCCTGACTCTAGGGTTCTAGCGCACATAGGAGATGATGATGTCACAGTAAGAATC AACACAGATGGGGCAGAATATAACATAGAGCCACTTTGGAGGTTTGTTAATGATACTAAAGATAAAAGGA TGCTGGTGTATAAGTCTGAAGATATCAAGGATTTTTCACGTTTGCAGTCTCCAAAAGTATGTGGTTATTT AAATGCAGATAGTGAAGAGTTGCTTCCAAAAGGGCTCATAGACAGAGAGCCATCTGAAGAGTTTGTCCGT CGAGTGAAGAGGCGAGCTGAACCTAACCCATTGAAGAATACTTGTAAATTACTGGTGGTGGCAGATCATC GATTTTATAAGTACATGGGCCGAGGAGAAGAGAGCACTACTACAAATTACTTAATAGAGTTAATTGACCG AGTTGATGACATATACCGGAACACCTCGTGGGACAATGCAGGATTTAAAGGTTATGGAGTACAGATAGAA CAGATTCGAATTCTCAAGTCTCCACAAGAGGTAAAACCTGGTGAAAGACACTTCAATATGGCAAAAAGTT TTCCAAATGAAGAAAAGGATGCTTGGGATGTGAAGATGCTGCTGGAGCAATTTAGCCTTGATATAGCTGA AGAGGCCTCTAAAGTCTGCCTGGCTCATCTTTTCACCTACCAAGATTTTGATATGGGAACTCTTGGATTA GCTTACGTTGGTTCTCCCAGAGCAAACAGTCATGGAGGGGTTTGTCCAAAAGCTTATTACAACCCAGGTG TGAAGAAGAACATCTATTTGAATAGTGGTCTGACAAGTACAAAAAATTATGGTAAAACCATCCTTACAAA GGAGGCTGACCTGGTTACAACTCATGAATTGGGACACAATTTTGGAGCAGAACATGATCCTGATGGGCTG GCAGAGTGTGCCCCAAATGAGGACCAAGGAGGAAAGTATGTTATGTACCCCATAGCTGTGAGTGGTGACC ATGAGAATAATAAGATGTTTTCAAACTGCAGTAAACAGTCCATCTACAAGACCATAGAAAGCAAGGCTCA AGAGTGCTTCCAGGAGCGCAGCAACAAGGTGTGCGGCAACTCCAGGGTGGACGAAGGAGAAGAGTGTGAC CCGGGCATCATGTACCTGAACAACGACACCTGCTGCAATAGTGACTGCACACTGAAGCCAGGTGTGCAGT GCAGTGATAGGAATAGTCCTTGCTGTAAAAATTGTCAGTTTGAGACGGCCCAGAAGAAGTGCCAGGAGGC TATCAATGCTACTTGCAAAGGAGTGTCTTACTGCACAGGGAATAGCAGTGAGTGCCCCCCACCAGGAGAT GCTGAAGATGACACTGTGTGCTTGGACCTGGGCAAGTGCAAGGCTGGGAAATGCATCCCTTTCTGCAAGA GGGAGCAGGAGCTGGAGTCCTGCGCATGTGCTGACACCGACAACTCGTGCAAGGTATGCTGCAGGAACCT TTCTGGCCCATGTGTGCCTTACGTCGATGCAGAGCAAAAGAACTTATTTTTGAGGAAAGGGAAGCCCTGT ACAGTAGGGTTTTGTGACATGAATGGCAAATGTGAGAAACGGGTACAGGACGTAATTGAGCGGTTTTGGG ATTTCATTGACCAGCTGAGCATCAACACTTTTGGGAAGTTTCTGGCAGACAACATCGTTGGGTCTGTTCT GGTTTTCTCCTTGATATTTTGGATTCCTTTCAGCATTCTTGTCCACTGTGTGGATAAGAAACTGGACAAG CAGTATGAGTCCCTGTCTCTGTTTCATCACAGTAACATTGAGATGTTGAGCAGCATGGACTCAGCATCTG TTCGCATTATCAAGCCGTTCCCTGCACCCCAGACTCCAGGTCGTCTGCAGGCCCTGCAGCCGGCTGCAAT GATGCCGCCAGTGTCTGCGGCTCCAAAACTGGATCACCAGAGGATGGACACCATCCAGGAAGACCCCAGC ACAGATTCACATGTGGATGATGATGGCTTTGAGAAGGACCCCTTCCCCAACAGCAGTGCAGCCGCCAAGT CCTTTGAGGACCTCACAGACCACCCAGTCACCAGGAGTGAGAAGGCCGCCTCCTTCAAGCTGCAGCGTCA GAGCCGAGTTGATAGCAAAGAGACAGAGTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214103 representing NM_020306
Red=Cloning site Green=Tags(s) MRQRLLFLTTLVPFVLAPRPPEEPGSGSHLRLEKLDSLLSDYDILSLSNIQQHSIRKRDLQSATHLETLL TFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQDFFSGHVVGEPDSRVLAHIGDDDVTVRI NTDGAEYNIEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVR RVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGVQIE QIRILKSPQEVKPGERHFNMAKSFPNEEKDAWDVKMLLEQFSLDIAEEASKVCLAHLFTYQDFDMGTLGL AYVGSPRANSHGGVCPKAYYNPGVKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGL AECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECD PGIMYLNNDTCCNSDCTLKPGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGD AEDDTVCLDLGKCKAGKCIPFCKREQELESCACADTDNSCKVCCRNLSGPCVPYVDAEQKNLFLRKGKPC TVGFCDMNGKCEKRVQDVIERFWDFIDQLSINTFGKFLADNIVGSVLVFSLIFWIPFSILVHCVDKKLDK QYESLSLFHHSNIEMLSSMDSASVRIIKPFPAPQTPGRLQALQPAAMMPPVSAAPKLDHQRMDTIQEDPS TDSHVDDDGFEKDPFPNSSAAAKSFEDLTDHPVTRSEKAASFKLQRQSRVDSKETEC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020306 |
| ORF Size | 2481 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020306.2, NP_064702.1 |
| RefSeq Size | 4126 bp |
| RefSeq ORF | 2484 bp |
| Locus ID | 57027 |
| UniProt ID | Q9Z1K9 |
| MW | 93 kDa |
| Gene Summary | expression occurs in response to oxygen glucose deprivation, may play a role in inhibition of apoptosis via induction of TNF-alpha release [RGD, Feb 2006] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ADAM17 promotes glioma cell malignant phenotype
,null,
Molecular carcinogenesis
,PubMed ID 21480393
[Adam17]
|
|
ADAM17 promotes breast cancer cell malignant phenotype through EGFR-PI3K-AKT activation
,null,
Cancer biology & therapy
,PubMed ID 19395875
[Adam17]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214103 | Adam17 (untagged ORF) - Rat ADAM metallopeptidase domain 17 (Adam17), (10 ug) |
CNY 10,550.00 |
|
| RR214103L3 | Lenti ORF clone of Adam17 (Myc-DDK-tagged ORF) - Rat ADAM metallopeptidase domain 17 (Adam17), (10 ug) |
CNY 12,450.00 |
|
| RR214103L4 | Lenti ORF clone of Adam17 (mGFP-tagged ORF) - Rat ADAM metallopeptidase domain 17 (Adam17), (10 ug) |
CNY 13,590.00 |
