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Uba1 (NM_001014080) Rat Tagged ORF Clone
CAT＃: RR211933
- TrueORF®
Uba1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug)
"NM_001014080" in other vectors (3)
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CNY 17,010.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ube1x |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR211933 representing NM_001014080
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCAGGTTCTAACTGCT CCTCTGCACAGTCAGTGCTGTCCGAAGTGTCTTCAGTGCCAACCAACGGAATGGCGAAGAACGGCAGTGA AGCAGACATAGACGAGAGCCTTTACTCCCGGCAGCTGTACGTTTTGGGCCATGAGGCAATGAAAATGCTC CAGACATCCAGTGTCCTCGTCTCAGGCTTGCGGGGCTTAGGTGTCGAGATTGCTAAGAACATCATCCTTG GTGGGGTCAAGGCTGTCACCCTACATGACCAAGGCACTACACAGTGGGCTGATCTCTCCTCCCAGTTTTA CCTTCGGGAGGAGGACATTGGTAAAAATCGAGCTGAGGTATCCCAGCCCCGACTTGCTGAACTCAACAGC TATGTACCTGTTACTGCCTACACTGGGCCTCTTGTTGAAGACTTCCTTAGTGGTTTCCAGGTGGTGGTCC TTACCAACAGCCCCCTGGAAGAACAGCTTCGAGTGGGTGAGTTCTGTCATAGCCGTGGCATCAAGCTAGT GGTGGCAGATACAAGAGGCCTATTTGGGCAACTTTTCTGTGATTTTGGAGAGGAAATGGTTCTCACAGAT TCCAATGGGGAGCAGCCACTCAGTGCTATGGTTTCAATGGTCACCAAGGACAACCCCGGTGTGGTTACCT GCCTTGATGAGGCCCGACATGGCTTTGAGACTGGTGACTTCGTCTCATTCTCAGAAGTACAGGGCATGGT CCAACTCAATGGATGTCAGCCCATAGAGATCAAAGTGCTGGGTCCTTATACTTTTAGTATCTGTGACACC TCCAACTTCTCTGACTACATCCGTGGAGGCATCGTCAGCCAGGTCAAAGTACCTAAAAAGATTAGCTTTA AATCCTTGCCAGCATCACTGGCAGAGCCTGACTTTGTGATGACTGACTTTGCCAAGTATTCTCGCCCTGC CCAACTGCACATTGGCTTTCAAGCTCTGCACCAATTCTGTGCTCAGCACAACCGACCACCTCGACCACGA AATGAGGAAGATGCAACAGAACTGGTGACCCTAGCTCAGGCTGTGAATGCTCGGTCCCCACCTGCAGTAC AACAGGACAACGTGGATGAAGACCTTATTCGGAAGCTAGCTTATGTTGCTGCTGGGGACCTGGCACCTAT AAATGCTTTCATTGGGGGCCTCGCTGCCCAGGAAGTCATGAAGGCCTGTTCTGGAAAGTTTATGCCCATC ATGCAGTGGTTGTACTTTGATGCTCTTGAATGTCTCCCAGAGGACAAAGAGGCTCTGACAGAGGACAAGT GCCTCCCACGTCAGAACCGTTATGATGGGCAGGTAGCTGTATTTGGCTCAGACCTTCAAGAGAAGCTGGG CAAGCAAAAGTACTTCCTGGTGGGTGCAGGGGCCATTGGCTGTGAATTGCTCAAGAACTTTGCCATGATT GGGTTAGGCTGTGGAGAGGGTGGAGAGGTCGTGGTCACAGACATGGACACCATTGAGAAATCGAATCTGA ACCGACAGTTTCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCCGCTGCAGCTGTGCG ACAGATGAATCCTTACATCCAGGTGACAAGCCACCAGAACCGTGTAGGCCCTGACACGGAGCGCATCTAT GATGATGATTTTTTCCAAAATTTGGATGGTGTGGCCAATGCACTGGACAATGTAGATGCCCGGATGTACA TGGATCGCCGCTGTGTGTACTATCGGAAGCCACTGCTAGAGTCTGGCACACTGGGCACAAAGGGCAATGT GCAGGTGGTGATCCCCTTCCTGACAGAATCATACAGCTCTAGCCAGGACCCACCAGAGAAATCCATCCCC ATTTGTACCCTGAAAAACTTTCCCAATGCCATCGAACACACTCTACAGTGGGCCCGGGATGAATTTGAAG GCCTTTTCAAGCAGCCAGCAGAAAATGTTAATCAGTACCTCACAGACTCCAAATTTGTGGAGCGGACATT GCGGCTGGCGGGTACCCAGCCATTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGACCA CAGACTTGGGGCGACTGTGTGACCTGGGCCTGCCACCACTGGCACACCCAGTACTGTAACAACATACGGC AGCTGCTGCACAACTTTCCTCCCGACCAGCTCACCAGCTCAGGGGCCCCTTTCTGGTCTGGACCCAAACG ATGTCCACACCCACTTACTTTTGATGTTAACAATACATTGCATCTGGATTATGTGATGGCTGCTGCCAAC CTTTTTGCCCAGACCTATGGGTTGACTGGCTCCCAAGACCGAGCTGCTGTGGCCTCACTCCTACAGTCAG TACAAGTTCCAGAGTTCACCCCCAAGTCTGGTGTCAAGATCCATGTTTCTGACCAGGAGTTGCAGAGCGC CAATGCCTCTGTTGATGACAGCCGTCTCGAGGAGCTCAAAGCCACACTGCCGAGCCCAGACAAGTTACCT GGATTTAAGATGTACCCCATTGATTTTGAAAAGGACGATGACAGCAATTTCCACATGGATTTCATTGTGG CTGCATCTAATCTTCGGGCCGAAAACTATGATATTTCCCCTGCAGACCGGCACAAGAGCAAGCTGATCGC AGGGAAGATCATCCCAGCTATCGCCACAACCACAGCTGCTGTGGTTGGCCTTGTGTGTCTGGAACTCTAC AAGGTAGTTCAGGGGCACCAACAACTTGATTCCTACAAAAATGGTTTCCTGAATTTGGCCCTGCCCTTCT TTGGGTTTTCGGAACCTCTTGCTGCGCCTCGTCACCAGTACTATAATCAAGAGTGGACATTGTGGGATCG CTTTGAAGTACAAGGGCTACAGCCTAATGGTGAGGAGATGACTCTCAAGCAGTTCCTTGATTACTTTAAG ACGGAGCACAAATTAGAGATCACCATGCTGTCCCAGGGTGTGTCCATGCTCTATTCTTTCTTCATGCCAG CTGCTAAGCTCAAGGAACGGTTGGATCAGCCGATGACAGAGATTGTGAGCCGAGTGTCAAAGAGAAAGCT GGGCCGCCATGTGCGTGCACTGGTGCTTGAGCTGTGCTGCAACGATGAAAGTGGCGAGGACGTCGAGGTC CCCTATGTACGATATACAATTCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211933 representing NM_001014080
Red=Cloning site Green=Tags(s) MSSSPLSKKRRVSGPDPKPGSNCSSAQSVLSEVSSVPTNGMAKNGSEADIDESLYSRQLYVLGHEAMKML QTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNS YVPVTAYTGPLVEDFLSGFQVVVLTNSPLEEQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTD SNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMVQLNGCQPIEIKVLGPYTFSICDT SNFSDYIRGGIVSQVKVPKKISFKSLPASLAEPDFVMTDFAKYSRPAQLHIGFQALHQFCAQHNRPPRPR NEEDATELVTLAQAVNARSPPAVQQDNVDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPI MQWLYFDALECLPEDKEALTEDKCLPRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMI GLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIY DDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIP ICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRP QTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAAN LFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELY KVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFK TEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEV PYVRYTIR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001014080 |
| ORF Size | 3174 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001014080.1, NP_001014102.1 |
| RefSeq Size | 3618 bp |
| RefSeq ORF | 3177 bp |
| Locus ID | 314432 |
| UniProt ID | Q5U300 |
| MW | 117.8 kDa |
| Gene Summary | Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN211933 | Uba1 (untagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug) |
CNY 17,010.00 |
|
| RR211933L3 | Lenti ORF clone of Uba1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug) |
CNY 18,620.00 |
|
| RR211933L4 | Lenti ORF clone of Uba1 (mGFP-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug) |
CNY 20,330.00 |
