Abcb1a (NM_133401) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Abcb1; Mdr1a
Vector	pCMV6-AC-Myc-DDK
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211714 representing NM_133401. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCTCGAAGAAGACCTTAACGGAAGAGCAGACAAGAACTTCTCAAAGATGGGCAAAAAGAGTAAA AAGGAGAAGAAAGAAAAGAAACCAGCGGTCAGTGTGCTCACAATGTTTCGCTATGCAGGTTGGCTGGAC AGATTTTACATGCTGCTGGGAACTCTGGCGGCCATTATCCATGGAATTGCGCTCCCACTTATGATGCTG GTCTTTGGAGACATGACAGATAGCTTTGCAAATGTAGGAAACAACCGTAGTATGAGTTTCTACAATGCT ACAGACATATATGCCAAGCTGGAGGACGAAATGACCACGTACGCCTACTATTACACGGGCATTGGTGCC GGTGTGCTCATCGTTGCCTACATCCAGGTTTCACTTTGGTGCCTGGCAGCTGGGAGACAAATACACAAG ATTAGGCAGAAGTTTTTCCATGCCATCATGAATCAGGAGATAGGCTGGTTTGACGTGCATGACGTTGGG GAGCTCAACACCCGGCTCACAGATGACGTCTCCAAAATTAATGAAGGAATTGGTGACAAAATTGGAATG TTCTTTCAGGCAATGGCAACATTTTTTGGTGGTTTTATAATAGGATTTACTCGCGGCTGGAAGCTAACT CTTGTGATTTTGGCCATCAGCCCTGTTCTTGGACTGTCAGCTGGTATTTGGGCAAAGATATTGTCTTCA TTTACTGATAAGGAACTCCAGGCTTATGCAAAAGCTGGAGCAGTTGCTGAAGAAGTCTTAGCAGCCATC AGAACTGTGATTGCCTTTGGAGGACAAAAGAAGGAACTTGAAAGGTACAATAACAATTTGGAAGAAGCT AAAAGGCTTGGGATAAAGAAAGCTATCACGGCCAACATTTCCATGGGTGCAGCTTTTCTGCTTATCTAT GCATCATATGCTCTGGCATTCTGGTATGGGACTTCCTTGGTCATCTCAAAAGAATACACTATTGGACAA GTGCTCACTGTCTTTTTTTCTGTATTAATTGGAGCATTCAGTGTTGGGCAGGCATCTCCAAATATTGAA GCCTTCGCCAATGCTAGAGGAGCAGCTTATGAAGTCTTCAGTATAATTGATAATAAGCCCAGTATAGAC AGCTTCTCAAAGAGTGGGCACAAACCCGACAACATACAAGGAAATTTGGAATTCAAAAATATTCACTTC AGTTACCCGTCTCGAAAAGACGTTCAGATCTTGAAGGGCCTCAACCTGAAGGTGAAGAGCGGGCAGACG GTAGCCCTGGTTGGCAACAGTGGCTGTGGGAAAAGCACAACTGTCCAGCTGCTGCAGAGGCTCTACGAC CCCATAGAGGGCGAGGTCAGTATCGACGGACAGGACATCAGGACCATCAATGTGAGGTATCTGCGGGAA ATCATTGGGGTGGTGAGTCAGGAACCCGTGCTGTTTGCCACCACAATTGCCGAAAACATTCGCTATGGC CGAGAAAACGTCACCATGGATGAGATAGAGAAAGCTGTCAAGGAAGCCAATGCCTATGATTTCATCATG AAACTGCCCCACAAATTTGACACCCTGGTTGGTGAGAGAGGGGCGCAGCTGAGTGGGGGACAGAAACAG AGGATCGCCATTGCCCGGGCCCTGGTCCGCAACCCCAAGATCCTTTTGTTGGATGAGGCCACGTCAGCC TTGGACACAGAAAGCGAAGCCGTGGTTCAGGCCGCTCTGGATAAGGCTAGAGAAGGCCGGACCACCATT GTGATAGCTCACCGCTTGTCTACAGTTCGCAATGCTGACGTCATTGCTGGTTTTGATGGTGGTGTCATT GTGGAGCAAGGAAATCATGATGAGCTCATGAGAGAGAAAGGAATTTACTTCAAACTTGTCATGACTCAG ACAGCAGGAAATGAAATTGAATTAGGAAATGAAGCTTGTGAATCTAAAGATGGAATTGATAATGTGGAC ATGTCTTCAAAAGATTCAGGATCCAGTCTAATAAGAAGAAGATCAACTCGCAAAAGCATCCGTGGGCCA CATGATCAAGACGGGGAACTTAGCACCAAAGAGGCTCTGGATGACGACGTACCTCCAGCTTCCTTTTGG CGGATCCTGAAGTTGAATTCAACTGAATGGCCTTATTTTGTGGTTGGTGTATTTTGTGCCATAATAAAT GGAGGCTTGCAACCAGCATTCTCCATAATATTTTCAAAGGTTGTAGGGGTTTTTACAAAAAATGACACC CCTGAAATCCAGCGGCAGAACAGCAACTTGTTTTCTTTATTGTTCCTGATCCTTGGGATCATCTCTTTC ATTACGTTTTTCCTTCAGGGCTTCACATTTGGCAAAGCTGGAGAGATCCTCACCAAGCGACTCCGATAC ATGGTCTTCAAATCCATGCTGAGACAGGACATAAGCTGGTTTGATGACCCTAAAAACACCACAGGAGCG CTGACCACCAGGCTTGCCAATGACGCTGCTCAAGTGAAAGGGGCTACAGGGTCTAGGCTTGCTGTTATT ACCCAGAACATAGCAAATCTTGGGACAGGCATCATCATATCCCTGATCTACGGCTGGCAATTGACACTT TTACTCCTAGCAATTGTTCCCATCATTGCTATAGCAGGAGTGGTTGAAATGAAAATGTTGTCTGGACAA GCGCTGAAAGATAAGAAGGAACTAGAAGGTTCTGGGAAGATCGCTACAGAAGCAATTGAAAACTTTCGC ACTGTCGTCTCTTTGACTCGGGAGCAGAAGTTTGAAACTATGTATGCCCAGAGCTTGCAGATACCATAC AGAAATGCTTTGAAGAAAGCGCACGTCTTTGGGATCACTTTCTCCTTCACCCAGGCCATGATGTATTTC TCCTATGCTGCTTGTTTCCGGTTTGGTGCCTACTTGGTGGCACGAGAACTCATGACATTTGAAAATGTT CTGTTAGTATTCTCAGCTATTGTCTTTGGTGCCATGGCAGTGGGGCAGGTCAGTTCATTCGCTCCTGAC TACGCGAAAGCCAAAGTCTCGGCATCCCACATCATCAGGATCATTGAGAAAATCCCTGAGATTGACAGC TACAGCACGGAGGGCTTGAAGCCTAATATGTTGGAAGGAAATGTGAAATTTAATGGAGTCATGTTCAAC TATCCCACCCGACCCAACATCCCAGTGCTTCAGGGGCTGAGCCTAGAGGTGAAGAAAGGGCAGACGCTG GCCCTCGTGGGCAGCAGTGGCTGCGGGAAGAGTACAGTGGTCCAGCTGCTTGAGCGCTTCTATGACCCC ATGGCCGGAACAGTGTTTCTAGATGGCAAAGAAATAAAGCAACTCAATGTCCAGTGGCTCCGCGCCCAC CTGGGCATTGTGTCCCAGGAGCCCATCCTGTTTGACTGCAGCATCGCCGAGAACATTGCCTACGGAGAC AACAGCCGTGTCGTGTCTCATGAGGAGATCGTGAAGGCAGCCAAGGAGGCCAACATCCACCAGTTCATC GACTCACTGCCTGAGAAATACAACACCAGAGTGGGAGACAAAGGGACTCAGCTGTCGGGCGGGCAGAAG CAGCGCATCGCCATCGCGCGCGCCCTCGTCAGACAGCCTCACATCTTACTTCTGGATGAAGCGACATCA GCTCTGGATACGGAGAGTGAAAAGGTCGTCCAGGAAGCGCTGGACAAAGCCAGGGAAGGCCGCACCTGC ATTGTGATCGCGCACCGCCTGTCCACCATCCAGAACGCAGACTTGATCGTGGTGATTCAGAACGGCCAG GTCAAGGAGCACGGCACCCACCAGCAGCTGCTGGCCCAGAAAGGCATCTATTTCTCGATGGTCAGTGTG CAGGCTGGAGCAAAGCGCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RR211714 Blue=ORF Red=Cloning site Green=Tag(s) MELEEDLNGRADKNFSKMGKKSKKEKKEKKPAVSVLTMFRYAGWLDRFYMLLGTLAAIIHGIALPLMML VFGDMTDSFANVGNNRSMSFYNATDIYAKLEDEMTTYAYYYTGIGAGVLIVAYIQVSLWCLAAGRQIHK IRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLT LVILAISPVLGLSAGIWAKILSSFTDKELQAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEA KRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYTIGQVLTVFFSVLIGAFSVGQASPNIE AFANARGAAYEVFSIIDNKPSIDSFSKSGHKPDNIQGNLEFKNIHFSYPSRKDVQILKGLNLKVKSGQT VALVGNSGCGKSTTVQLLQRLYDPIEGEVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYG RENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSA LDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQ TAGNEIELGNEACESKDGIDNVDMSSKDSGSSLIRRRSTRKSIRGPHDQDGELSTKEALDDDVPPASFW RILKLNSTEWPYFVVGVFCAIINGGLQPAFSIIFSKVVGVFTKNDTPEIQRQNSNLFSLLFLILGIISF ITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDISWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVI TQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFR TVVSLTREQKFETMYAQSLQIPYRNALKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVARELMTFENV LLVFSAIVFGAMAVGQVSSFAPDYAKAKVSASHIIRIIEKIPEIDSYSTEGLKPNMLEGNVKFNGVMFN YPTRPNIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVFLDGKEIKQLNVQWLRAH LGIVSQEPILFDCSIAENIAYGDNSRVVSHEEIVKAAKEANIHQFIDSLPEKYNTRVGDKGTQLSGGQK QRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGQ VKEHGTHQQLLAQKGIYFSMVSVQAGAKRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133401
ORF Size	3816 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133401.1, NP_596892.1
RefSeq Size	4927 bp
RefSeq ORF	3819 bp
Locus ID	170913
MW	140.3 kDa
Gene Summary	ATP-binding cassette (ABC) multiple drug transporter P-glycoprotein that is activated during liver regeneration and hepatocarcinogenesis [RGD, Feb 2006]

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