Aox1 (NM_019363) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211283 representing NM_019363 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCCCCGCAGCTGCTCTTCTACGTGAATGGCCAGAAGGTGGTAGAAAACAATGTTGATCCTGAGA TGATGCTTTTACCGTACCTGAGGAAGAACCTCCGACTCACGGGAACTAAGTATGGCTGTGGAGGCGGCGG CTGCGGTGCCTGCACAGTGATGATCTCACGGTACAACCCCAGCACCAAGAGCATCAGGCATCATCCTGTC AATGCCTGTCTGACCCCCATCTGCTCTCTGTACGGTACAGCAGTCACCACAGTAGAGGGCATAGGCAACA CCAGGACCAGACTTCATCCTGTTCAGGAGAGGATCGCCAAGTGTCACAGCACCCAGTGTGGGTTCTGTAC CCCTGCGAGGGTGATGTCCATGTATGCTCTGCTCAGGAACCACCCAGAGCCCTCTCTAGATCAGTTAACT GATGCCCTTGGGGGGAATCTGTGCCGCTGTACCGGATACAGGCCCATAATTGACGCTTGCAAGACTTTCT GTAGAGCTTCTGGTTGCTGTGAAAGTAAAGAAAATGGGGTGTGCTGTTTGGATCAAGGAATAAATGGATC GGCAGAATTTCAGGAAGGAGATGAGACAAGTCCAGAACTGTTCTCGGAAAAGGAATTTCAGCCACTGGAC CCAACCCAAGAGCTGATATTTCCTCCAGAGCTAATGAGAATAGCCGAGAAACAGCCACCAAAGACCAGAG TGTTCTACAGTAATAGAATGACATGGATCTCCCCGGTGACCCTGGAGGAACTTGTGGAAGCTAAGTTCAA GTATCCTGGGGCCCCCATTGTCATGGGGTACACCTCTGTGGGGCCTGAAGTAAAGTTTAAAGGTGTCTTC CACCCCATCATAATTTCTCCTGACAGAATTGAAGAGCTGAGTATCATAAACCAGACTGGGGATGGGCTGA CCCTGGGTGCTGGCCTCAGCCTGGACCAGGTGAAGGACATTCTCACTGACGTGGTCCAGAAGCTTCCAGA AGAGACGACACAGACATACCGTGCGCTCCTGAAGCACCTGAGAACTCTGGCTGGCTCTCAGATCAGGAAT ATGGCTTCTTTAGGGGGCCACATCGTGAGCAGACATCTGGACTCGGATCTGAATCCCCTTCTGGCTGTGG GTAATTGTACCCTCAACTTACTATCCAAAGATGGAAAACGGCAGATCCCTTTAAGTGAGCAGTTTCTCCG CAAGTGTCCTGACTCGGATCTTAAGCCTCAGGAAGTCTTGGTCTCAGTGAACATCCCCTGTTCCAGGAAG TGGGAGTTTGTGTCAGCCTTCCGACAAGCCCAGAGACAGCAGAATGCACTAGCGATTGTCAACTCTGGAA TGAGAGTCCTTTTTAGAGAAGGAGGTGGCGTCATTAAAGAGTTATCCATTTTGTATGGAGGTGTCGGTCC AACCACCATCGGTGCCAAGAACTCCTGTCAGAAACTCATTGGAAGGCCCTGGAATGAAGAGATGCTGGAC ACAGCATGCAGGCTGGTTTTGGATGAAGTCACCCTTGCAGGTTCAGCTCCTGGTGGGAAGGTGGAGTTCA AGAGGACCCTCATCATCAGCTTCCTTTTCAAGTTCTACCTGGAGGTGCTGCAGGGTCTGAAGAGGGAGGA CCCAGGTCACTATCCTAGCTTGACAAACAATTATGAGAGTGCTTTAGAAGATCTCCATTCAAAACATCAC TGGAGAACATTAACCCACCAGAACGTGGATTCGATGCAGCTTCCTCAGGACCCAATTGGCCGTCCCATCA TGCACCTTTCTGGTATTAAGCACGCTACCGGCGAGGCCATCTACTGTGACGACATGCCTGCAGTAGACCG GGAGCTTTTCCTGACTTTTGTAACAAGTTCAAGAGCACACGCTAAGATTGTGTCCATTGATCTGTCAGAA GCTCTCAGCCTGCCAGGCGTGGTGGACATCATTACTGCGGATCATCTTCAGGACACAACCACCTTCGGCA CAGAGACGCTTCTGGCCACAGATAAGGTCCACTGCGTGGGCCAACTTGTCTGTGCCGTGATTGCGGATTC TGAGACACGGGCAAAGCAAGCGGCGAAGCACGTGAAGGTGGTCTACCGAGACTTGGAGCCTCTGATCCTA ACTATTGAGGAAGCTATACAACACAAGTCCTTCTTCGAGTCAGAACGGAAGCTGGAGTGTGGAAATGTTG ATGAAGCGTTTAAAATTGCTGATCAAATTCTTGAAGGTGAGATACACATAGGCGGCCAGGAACATTTTTA TATGGAAACCCAAAGCATGCTTGTTGTCCCCAAAGGAGAGGACGGAGAGATTGACATCTATGTGTCCACA CAGTTCCCCAAGCATATACAGGATATAGTTGCTGCAACCTTGAAGCTTTCAGTCAACAAGGTCATGTGTC ATGTAAGGCGTGTTGGTGGGGCGTTCGGAGGGAAGGTAGGCAAGACCAGCATCATGGCGGCCATCACTGC ATTCGCTGCCAGCAAACACGGTCGCGCGGTCCGCTGCACTCTGGAACGAGGGGAAGACATGTTGATAACT GGGGGCCGCCATCCTTACCTTGGAAAGTATAAAGTTGGATTCATGAGGGACGGCAGAATCGTGGCCCTGG ATGTGGAGCACTATTGCAATGGAGGGAGCTCCCTGGATGAGTCCTTATGGGTGATAGAAATGGGGCTTCT GAAGATGGACAACGCTTACAAGTTTCCCAATCTACGCTGCCGGGGCTGGGCCTGCAGAACCAACCTCCCG TCCCACACTGCTCTGCGTGGGTTTGGCTTTCCTCAGGCAGGGCTGGTCACCGAAGCCTGTGTCACAGAAG TGGCAATCAGATGTGGCCTGTCTCCTGAGCAGGTTCGAACCATAAATATGTACAAGCAAATTGATAATAC CCATTACAAGCAAGAATTCAGCGCCAAGACCCTCTTTGAGTGCTGGAGAGAATGCATGGCCAAGTGTTCC TACTCTGAGAGGAAAACGGCTGTAGGAAAATTCAATGCAGAGAATTCCTGGAAGAAGAGGGGAATGGCCG TGATTCCATTGAAGTTTCCTGTGGGTGTTGGCTCAGTAGCCATGGGACAGGCGGCTGCCCTGGTCCATAT TTATCTTGATGGCTCTGCACTGGTCTCTCACGGTGGGATTGAGATGGGGCAGGGTGTCCACACTAAAATG ATCCAGGTGGTCAGCCGGGAATTAAAGATGCCAATGTCGAGTGTCCACCTGCGTGGGACAAGCACAGAAA CCGTCCCCAACACCAATGCATCTGGAGGCTCTGTGGTGGCAGATCTCAATGGATTGGCAGTAAAGGATGC TTGCCAGACCCTTCTAAAACGCCTTGAGCCCATCATCAGCAAGAACCCCCAGGGAACTTGGAAGGATTGG GCCCAGACTGCTTTTGACCAGAGCGTCAGTCTCTCGGCTGTTGGATATTTCAGGGGCTACGAGTCGAATA TAAACTGGGAGAAAGGGGAAGGCCATCCCTTCGAATACTTTGTGTATGGAGCTGCCTGCTCAGAGGTTGA AATAGACTGCCTGACCGGGGACCATAAGAATATCAGAACAGACATCGTGATGGATGTTGGCCACAGCATA AACCCAGCCCTTGACATAGGCCAGGTTGAAGGTGCATTTATTCAAGGAATGGGACTTTACACGATAGAGG AGCTGAGCTACTCTCCTCAGGGCATTCTGTACAGTCGTGGTCCAAACCAGTACAAGATCCCTGCCATCTG CGACATCCCCACCGAGATGCACATTTCTTTTTTGCCCCCATCCGAACACTCAAACACCCTGTATTCATCT AAGGGTCTGGGAGAGTCTGGGGTGTTCCTGGGCTGTTCGGTATTTCTTGCCATCCATGACGCAGTGAGGG CAGCGCGGCAGGAGAGAGGCATCTCTGGACCATGGAAGCTCACTAGTCCTCTGACTCCAGAGAAAATCAG AATGGCTTGTGAAGATAAGTTCAGAAAAATGATTCCAAGAGATGAACCTGGATCCTATGTTCCCTGGAAC ATACCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211283 representing NM_019363 Red=Cloning site Green=Tags(s) MDPPQLLFYVNGQKVVENNVDPEMMLLPYLRKNLRLTGTKYGCGGGGCGACTVMISRYNPSTKSIRHHPV NACLTPICSLYGTAVTTVEGIGNTRTRLHPVQERIAKCHSTQCGFCTPARVMSMYALLRNHPEPSLDQLT DALGGNLCRCTGYRPIIDACKTFCRASGCCESKENGVCCLDQGINGSAEFQEGDETSPELFSEKEFQPLD PTQELIFPPELMRIAEKQPPKTRVFYSNRMTWISPVTLEELVEAKFKYPGAPIVMGYTSVGPEVKFKGVF HPIIISPDRIEELSIINQTGDGLTLGAGLSLDQVKDILTDVVQKLPEETTQTYRALLKHLRTLAGSQIRN MASLGGHIVSRHLDSDLNPLLAVGNCTLNLLSKDGKRQIPLSEQFLRKCPDSDLKPQEVLVSVNIPCSRK WEFVSAFRQAQRQQNALAIVNSGMRVLFREGGGVIKELSILYGGVGPTTIGAKNSCQKLIGRPWNEEMLD TACRLVLDEVTLAGSAPGGKVEFKRTLIISFLFKFYLEVLQGLKREDPGHYPSLTNNYESALEDLHSKHH WRTLTHQNVDSMQLPQDPIGRPIMHLSGIKHATGEAIYCDDMPAVDRELFLTFVTSSRAHAKIVSIDLSE ALSLPGVVDIITADHLQDTTTFGTETLLATDKVHCVGQLVCAVIADSETRAKQAAKHVKVVYRDLEPLIL TIEEAIQHKSFFESERKLECGNVDEAFKIADQILEGEIHIGGQEHFYMETQSMLVVPKGEDGEIDIYVST QFPKHIQDIVAATLKLSVNKVMCHVRRVGGAFGGKVGKTSIMAAITAFAASKHGRAVRCTLERGEDMLIT GGRHPYLGKYKVGFMRDGRIVALDVEHYCNGGSSLDESLWVIEMGLLKMDNAYKFPNLRCRGWACRTNLP SHTALRGFGFPQAGLVTEACVTEVAIRCGLSPEQVRTINMYKQIDNTHYKQEFSAKTLFECWRECMAKCS YSERKTAVGKFNAENSWKKRGMAVIPLKFPVGVGSVAMGQAAALVHIYLDGSALVSHGGIEMGQGVHTKM IQVVSRELKMPMSSVHLRGTSTETVPNTNASGGSVVADLNGLAVKDACQTLLKRLEPIISKNPQGTWKDW AQTAFDQSVSLSAVGYFRGYESNINWEKGEGHPFEYFVYGAACSEVEIDCLTGDHKNIRTDIVMDVGHSI NPALDIGQVEGAFIQGMGLYTIEELSYSPQGILYSRGPNQYKIPAICDIPTEMHISFLPPSEHSNTLYSS KGLGESGVFLGCSVFLAIHDAVRAARQERGISGPWKLTSPLTPEKIRMACEDKFRKMIPRDEPGSYVPWN IPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019363
ORF Size	3999 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019363.3, NP_062236.2
RefSeq Size	4303 bp
RefSeq ORF	4002 bp
Locus ID	54349
UniProt ID	Q9Z0U5
MW	147 kDa
Gene Summary	Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. Plays a role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Participates in the bioactivation of prodrugs such as famciclovir, catalyzing the oxidation step from 6-deoxypenciclovir to penciclovir, which is a potent antiviral agent. Is probably involved in the regulation of reactive oxygen species homeostasis. Is a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. Also catalyzes nitric oxide (NO) production; under anaerobic conditions, reduces nitrite to NO with NADH or aldehyde as electron donor, but under aerobic conditions, NADH is the preferred substrate. These reactions may be catalyzed by several isozymes. May play a role in adipogenesis.[UniProtKB/Swiss-Prot Function]

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