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Yars (NM_001025696) Rat Tagged ORF Clone
CAT＃: RR210925
- TrueORF®
Yars (Myc-DDK-tagged ORF) - Rat tyrosyl-tRNA synthetase (Yars), (10 ug)
"NM_001025696" in other vectors (3)
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CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC112837 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210925 representing NM_001025696
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAACCTGACGGGACTAGCGTGACAGTTCCCGGCACGCGGAGACGGCGGCGAACGAGGAAAGGGCGCG GGTGCCGGCTGAGCGCGGGAAACCGAGAGAGCGGAGCCATGGGGGATGCTCCAAGCCCTGAGGAGAAGCT ACACCTTATCACCCGGAACCTGCAGGAGGTTCTGGGGGAAGAGAAGCTGAAGGAGATACTGAAGGAGCGG GAACTTAAAGTTTACTGGGGCACGGCTACCACCGGGAAGCCACACGTGGCCTACTTTGTACCCATGTCCA AGATTGCTGACTTCTTGAAGGCAGGGTGTGAGGTAACCATCCTGTTTGCAGACCTTCATGCATACCTGGA CAACATGAAAGCTCCCTGGGAACTTCTAGAACTTCGAACCAGTTACTACGAAAATGTGATCAAGGCAATG CTGGAGAGTATTGGTGTGCCCTTGGAGAAGCTCAAGTTTACCAAAGGCACCGACTACCAGCTCAGCAAAG AGTACACACTGGATGTGTACCGGCTGTCCTCCCTGGTCACACAACATGATGCCAAGAAAGCCGGGGCTGA GGTCGTAAAGCAGGTGGAGCACCCTTTGCTGAGTGGTCTGTTGTATCCCGGTCTGCAGGCCTTAGACGAA GAGTACTTAAAAGTAGATGCCCAGTTTGGCGGTATTGATCAGAGGAAGATTTTTACCTTTGCAGAGAAGT ATCTCCCCACACTTGGCTACTCAAAACGGGTCCATCTGATGAACCCCATGGTTCCTGGGTTAACTGGGAG CAAAATGAGCTCATCAGAAGAGGAGTCCAAAATCGACCTCCTTGATCGGAAGGAGGATGTGAAGAAAAAG CTGAAGAAGGCCTTCTGTGAGCCCGGCAACGTGGAGAACAATGGGGTGCTGTCCTTCGTCAAGCACGTCC TCTTTCCTCTCAAGTCTGAGTTTGTGATCCTGAGAGATGAGAAGTGGGGTGGAAACAAAACCTACACCAT TTACCAGGAGCTGGAAAAGGACTTTGCTGCTGAGGTTGTACACCCTGGAGACCTAAAGAATTCTGTTGAA GTCGCCCTGAACAAGTTGCTGGACCCCATTCGAGAAAAGTTTAATACCCCAGCCCTGAAGAAGCTGGCCA GTGCTGCCTACCCTGATCCTTCAAAGCAGAAGCCTACTGCCAAAGGCCCTGCCAAGAGTTCAGAACCAGA GGAGATCATCCCATCCCGGCTGGACATCCGTGTGGGCAAAATCCTCAGCGTGGAAAAGCACCCAGATGCA GATAGCCTGTATGTGGAGAAGATTGATGTGGGGGAAGCCGAACCCAGGACTGTGGTGAGCGGCCTCGTGC AGTTTGTGCCCAAAGAAGAACTACAGGACCGACTGGTGGTGGTGCTGTGCAACCTGAAACCCCAGAAGAT GAGAGGAGTGGACTCCCAGGGCATGCTACTGTGTGCTTCTGTAGAAGGGGTAAGCCGCCAGGTGGAACCT CTGGACCCTCCTGCAGGCTCTGCCCCTGGTGAACGGGTATTTGTACAGGGCTATGAGAAGGGCCAACCTG ACGAGGAGCTCAAGCCCAAGAAGAAAGTCTTTGAGAAGCTGCAGGCCGACTTTAAGATTTCTGATGACTG CGTCGCACAGTGGAAGCAAACCAACTTCATGACCAAGCTGGGCTTTGTCTCCTGTAAATCACTAAAAGGG GGCAACATCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210925 representing NM_001025696
Red=Cloning site Green=Tags(s) MQPDGTSVTVPGTRRRRRTRKGRGCRLSAGNRESGAMGDAPSPEEKLHLITRNLQEVLGEEKLKEILKER ELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRTSYYENVIKAM LESIGVPLEKLKFTKGTDYQLSKEYTLDVYRLSSLVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDE EYLKVDAQFGGIDQRKIFTFAEKYLPTLGYSKRVHLMNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKK LKKAFCEPGNVENNGVLSFVKHVLFPLKSEFVILRDEKWGGNKTYTIYQELEKDFAAEVVHPGDLKNSVE VALNKLLDPIREKFNTPALKKLASAAYPDPSKQKPTAKGPAKSSEPEEIIPSRLDIRVGKILSVEKHPDA DSLYVEKIDVGEAEPRTVVSGLVQFVPKEELQDRLVVVLCNLKPQKMRGVDSQGMLLCASVEGVSRQVEP LDPPAGSAPGERVFVQGYEKGQPDEELKPKKKVFEKLQADFKISDDCVAQWKQTNFMTKLGFVSCKSLKG GNIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025696 |
| ORF Size | 1692 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025696.1, NP_001020867.2 |
| RefSeq Size | 2466 bp |
| RefSeq ORF | 1695 bp |
| Locus ID | 313047 |
| UniProt ID | Q4KM49 |
| MW | 63 kDa |
| Gene Summary | Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210925 | Yars (untagged ORF) - Rat tyrosyl-tRNA synthetase (Yars), (10 ug) |
CNY 6,750.00 |
|
| RR210925L3 | Lenti ORF clone of Yars (Myc-DDK-tagged ORF) - Rat tyrosyl-tRNA synthetase (Yars), (10 ug) |
CNY 8,740.00 |
|
| RR210925L4 | Lenti ORF clone of Yars (mGFP-tagged ORF) - Rat tyrosyl-tRNA synthetase (Yars), (10 ug) |
CNY 9,600.00 |
