Top3b (NM_001105861) Rat Tagged ORF Clone

CAT＃: RR207379

  • TrueORF®

Top3b (Myc-DDK-tagged ORF) - Rat topoisomerase (DNA) III beta (Top3b), (10 ug)

Datasheet
SDS
Plasmid Map

  "NM_001105861" in other vectors (3)

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CNY 11,120.00

货期*
7周
规格
    • 10 ug

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR207379 representing NM_001105861
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACGGTGCTCATGGTAGCAGAAAAGCCATCCCTGGCACAATCCATTGCCAAAATCCTCTCCCGAG
GAAACATGTCCTCACACAAAGGGCTAAATGGGGCGTGTTCAGTGCACAAATACACAGGAACCTTTGCTGG
CCAGCCCGTCCACTTCAAGATGACATCTGTCTGTGGTCATGTGATGACACTGGATTTTCTGGGAAAGTAC
AACAAGTGGGATAAGGTAGATCCTGCAGAGTTATTCAGCCAAGCTCCAACAGAGAAAAAGGAAGCAAACC
CCAAGCTGAACATGGTCAAGTTCCTGCAGGTGGAGGGCAGAGGCTGTGACTACGTCGTGCTGTGGCTGGA
CTGTGACAAGGAAGGCGAGAATATTTGCTTTGAGGTCCTTGATGCCGTTCTGCCTGTCCTGAATAATGCC
CACAATGGTGAGAAGACAGTGTTCCGGGCCAGGTTCAGTTCCATCACAGACACGGACATCTGCAATGCCA
TGACCCGCCTGACTGAGCCAGACCACAATGAGGCACTCTCGGTGGATGCCCGCCAGGAGCTAGACCTGCG
CATCGGCTGTGCGTTCACTAGGTTTCAAACAAAGTATTTCCAGGGAAAATATGGCGACTTAGACAGCTCT
CTCATCTCCTTTGGGCCTTGTCAGACTCCAACCCTGGGATTCTGTGTAGAAAGACATGATAAAATCCAGT
CCTTCAAACCAGAGACCTACTGGGTACTTCAGGCCAAGGTTCATACTGATAAAGAGGAGTCACTCCTTTT
GGACTGGGACCGAGTTAGAGTTTTCGACCGGGAGATTGCACAGATGTTTTTAAACATGACAAAGCTAGAA
AAAGAAGCTTGGGTGGAGGCTACAAGCAAGAAAGAAAAAGCCAAGCAGAGACCACTTGCTCTGAACACTG
TGGAGATGTTGCGTGTGGCCAGTTCAGCTCTGGGTATGGGACCACAGCATGCCATGCAAGTAGCTGAGAG
ATTATACACACAGGGCTACATCAGCTACCCACGGACAGAAACCACACACTATCCCGAGAACTTTGACCTG
AAGGGCTCTTTACGACAACAGGCCAATCACCCATACTGGGCAGACTCGGTAAAGCAGTTGTTAGCAGAAG
GCATCAACCGCCCTCGGAAAGGTCACGATGCTGGTGACCATCCTCCGATCACACCAATGAAGTCAGCTAC
AGAGGCTGAATTAGGAGGTGACGCTTGGCGACTCTACGAGTACATCACCAGACACTTCATCGCCACAGTG
AGCCATGACTGCAAGTATCTCCAGAGCACCATCTCCTTCAGGATCGGGCCCGAGCACTTCACATGCATGG
GAAAGACAGTTATTTCCCCAGGCTTCACAGAGATCATGCCTTGGCAGAGCGTGCCCCTGGAAGAGAGTCT
GCCCACATGCCAGAAGGGCGATACCTTCACTGTGGGTGAGGTGAAGATGCTAGAGAAGCAGACGAGCCCC
CCGGACTATCTGACTGAGGCTGAGCTCATCACCCTCATGGAGAAGCATGGCATCGGGACGGATGCCAGTA
TCCCTGTGCACATCAACAACATCTGCCAGCGCAACTACGTCACAGTGGAGAACGGCCGCCGGCTCAAGCC
CACCAACCTCGGCATAGTTCTGGTGCACGGCTACCACAAGATTGATGCAGAGCTGGTGCTCCCCACCATC
CGCAGCGCAGTGGAGAAGCAGCTGAACCTTATTGCCCAGGGCAAAGCCAACTACCACCAGGTGCTGGGCC
ACACCCTGGATATCTTCAAGAGAAAGTTCCACTACTTTGTTGATTCTATTGCTGGCATGGACGAGTTAAT
GGAGGTATCCTTTTCACCCCTGGCTGCCACTGGCAAGCCCCTCTCGCGCTGTGGGAAGTGCCATCGATTT
ATGAAGTATATCCAGGCCAAGCCAAGCCGCCTGCACTGCTCCCACTGCGATGAGACCTACACCCTCCCCC
AGAATGGTACCATCAAGCTCTACAAGGAGCTGCGGTGCCCATTGGATGACTTTGAACTGGTCTTGTGGTC
CTCAGGCTCCCGGGGCAAGAGCTACCCCCTCTGTCCCTACTGCTATAACCACCCACCCTTCCGAGACATG
AAGAAAGGCATGGGCTGCAATGAGTGCACACACCCTACGTGCCAGCACTCACTGAGTATGCTGGGCATTG
GCCAGTGCGTGGAGTGCGAGAATGGAGTCCTGGTTCTGGACCCCACCTCCGGTCCCAAGTGGAAGGTGGC
CTGCAATACATGCAGCGTAGTGGCACACTGCTTTGACAATGCCCACCGAGTACGGGTGTCTGCTGACACC
TGCAACACCTGCGAGGCTGCCTTGCTCGATGTAGACTTCAACAAGGCCAAGTCTCCCCTTCCTGGCAACG
AGACACAGCACACAGGCTGCATCTTCTGTGACACCGTCTTCCAGGAGCTGGTGGAGCTGAAGCATGCAGC
CTCCTGCCACCCCATGCACCGAGGTGGTCCTGGAAGGAGGCAGGGTCGTGGGCGGGCCCGGGGCCGGAGA
CCTCCAGGAAAGCCCAACCCCAGGCGGCCAAAAGACAAGATGTCAGCCCTGGCTGCTTACTTTGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RR207379 representing NM_001105861
Red=Cloning site Green=Tags(s)

MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAGQPVHFKMTSVCGHVMTLDFLGKY
NKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEGENICFEVLDAVLPVLNNA
HNGEKTVFRARFSSITDTDICNAMTRLTEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSS
LISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVHTDKEESLLLDWDRVRVFDREIAQMFLNMTKLE
KEAWVEATSKKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQVAERLYTQGYISYPRTETTHYPENFDL
KGSLRQQANHPYWADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATV
SHDCKYLQSTISFRIGPEHFTCMGKTVISPGFTEIMPWQSVPLEESLPTCQKGDTFTVGEVKMLEKQTSP
PDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVENGRRLKPTNLGIVLVHGYHKIDAELVLPTI
RSAVEKQLNLIAQGKANYHQVLGHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRF
MKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDM
KKGMGCNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCSVVAHCFDNAHRVRVSADT
CNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDTVFQELVELKHAASCHPMHRGGPGRRQGRGRARGRR
PPGKPNPRRPKDKMSALAAYFV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001105861
ORF Size 2586 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001105861.1, NP_001099331.1
RefSeq Size 2966 bp
RefSeq ORF 2589 bp
Locus ID 287930
MW 96.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RN207379 Top3b (untagged ORF) - Rat topoisomerase (DNA) III beta (Top3b), (10 ug)
    • 10 ug

CNY 11,120.00

RR207379L3 Lenti ORF clone of Top3b (Myc-DDK-tagged ORF) - Rat topoisomerase (DNA) III beta (Top3b), (10 ug)
    • 10 ug

CNY 12,830.00

RR207379L4 Lenti ORF clone of Top3b (mGFP-tagged ORF) - Rat topoisomerase (DNA) III beta (Top3b), (10 ug)
    • 10 ug

CNY 14,060.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它Top3b产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
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RR207379L3

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