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Abi1 (NM_024397) Rat Tagged ORF Clone
CAT＃: RR206292
- TrueORF®
Abi1 (Myc-DDK-tagged ORF) - Rat abl-interactor 1 (Abi1), (10 ug)
"NM_024397" in other vectors (3)
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CNY 6,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E3b1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206292 representing NM_024397
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGCTGCAGATGTTACTAGAGGAGGAGATCCCGTCTGGCAAGAGGGCGCTGATAGAGAGTTACC AGAACCTGACCCGGGTGGCGGACTACTGTGAAAACAACTACATACAGGCTACAGACAAGAGGAAAGCTCT AGAAGAGACCAAAGCATATACAACTCAATCTCTAGCTAGTGTTGCTTATCAAATAAATGCATTGGCCAAC AATGTGCTCCAGCTGCTGGATATCCAGGCCTCTCAGCTGCGGAGGATGGAGTCGTCCATCAATCACATCT CACAGACTGTGGATATTCATAAAGAAAAAGTGGCTCGAAGAGAGATTGGTATTTTGACAACAAATAAGAA TACATCAAGAACTCATAAAATAATCGCACCTGCAAATATGGAGCGTCCTGTCAGGTATATTCGGAAACCT ATTGACTACACAGTTCTGGATGATGTGGGCCATGGAGTTAAGTGGCTAAAAGCCAAGCACGGAAATAACC AGCCTGCAAGAACTGGCACATTGTCGAGAACAAATCCTCCCACGCAGAAACCACCAAGCCCTCCTGTGTC AGGCCGAGGAACTTTGGGGCGGAATACCCCTTACAAAACCCTAGAGCCTGTTAAGCCTCCCACAGTTCCT AATGACTACATGACTAGTCCTGCGAGGCTTGGAAGCCAGCATAGTCCAGGCAGGACGGCTTCTTTAAATC AGAGACCAAGGACGCATAGTGGGAGTAGTGGAGGAAGCGGAAGCCGGGAAAACAGTGGGAGCAGCAGCAT TGGTATTCCCATTGCTGTGCCCACACCCTCCCCGCCCACTGCGGGCCCAGCAGCCCCTGGCGCAGCTCCT GGTTCCCAGTATGGCACAATGACCAGGCAGATTTCTCGACACAACTCTACCACTTCTTCGACATCTTCTG GTGGATATAGACGAACTCCCTCTGTGACTGCGCAATTCTCTGCTCAGCCTCATGTTAATGGAGGTCCACT TTATTCTCAAAATTCAATTTCTATTGCCCCCCCTCCTCCCCCCATGCCTCAGTTGACTCCACAGATACCT CTCACAGGCTTCGTGGCCAGGGTGCAGGAAAACATTGCTGATAGCCCAACTCCACCACCACCTCCTCCAC CAGATGACATTCCCATGTTCGATGACTCTCCACCTCCCCCACCACCTCCTCCTGTGGACTACGAAGACGA AGAAGCTGCAGTAGTTCAGTATAGTGACCCATATGCAGATGGGGACCCTGCATGGGCTCCCAAGAACTAT ATTGAGAAAGTTGTTGCAATATATGATTATACAAAAGACAAGGATGATGAGCTGTCCTTTAAAGAGGGTG CAATCATCTATGTCATAAAGAAGAACGATGATGGCTGGTTCGAAGGAGTTTGCAATCGAGTAACTGGACT CTTCCCTGGGAACTATGTTGAATCAATCATGCACTATACTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206292 representing NM_024397
Red=Cloning site Green=Tags(s) MAELQMLLEEEIPSGKRALIESYQNLTRVADYCENNYIQATDKRKALEETKAYTTQSLASVAYQINALAN NVLQLLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANMERPVRYIRKP IDYTVLDDVGHGVKWLKAKHGNNQPARTGTLSRTNPPTQKPPSPPVSGRGTLGRNTPYKTLEPVKPPTVP NDYMTSPARLGSQHSPGRTASLNQRPRTHSGSSGGSGSRENSGSSSIGIPIAVPTPSPPTAGPAAPGAAP GSQYGTMTRQISRHNSTTSSTSSGGYRRTPSVTAQFSAQPHVNGGPLYSQNSISIAPPPPPMPQLTPQIP LTGFVARVQENIADSPTPPPPPPPDDIPMFDDSPPPPPPPPVDYEDEEAAVVQYSDPYADGDPAWAPKNY IEKVVAIYDYTKDKDDELSFKEGAIIYVIKKNDDGWFEGVCNRVTGLFPGNYVESIMHYTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024397 |
| ORF Size | 1443 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024397.2, NP_077373.2 |
| RefSeq Size | 3009 bp |
| RefSeq ORF | 1446 bp |
| Locus ID | 79249 |
| MW | 52.3 kDa |
| Gene Summary | May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206292 | Abi1 (untagged ORF) - Rat abl-interactor 1 (Abi1), (10 ug) |
CNY 6,270.00 |
|
| RR206292L3 | Lenti ORF clone of Abi1 (Myc-DDK-tagged ORF) - Rat abl-interactor 1 (Abi1), (10 ug) |
CNY 8,080.00 |
|
| RR206292L4 | Lenti ORF clone of Abi1 (mGFP-tagged ORF) - Rat abl-interactor 1 (Abi1), (10 ug) |
CNY 8,840.00 |
