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P4ha1 (NM_172062) Rat Tagged ORF Clone
CAT＃: RR206083
- TrueORF®
P4ha1 (Myc-DDK-tagged ORF) - Rat procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I (P4ha1), (10 ug)
"NM_172062" in other vectors (3)
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CNY 6,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PHalphaI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206083 representing NM_172062
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTGGGGTGTATTAATGATGGGGATTCTACTTCCTCAGTGTTCAGCCCATCCAGGCTTTTTTACTT CAATTGGTCAGATGACTGACTTGATCCATAATGAGAAAGACCTGGTGACGTCACTAAAAGATTACATTAA AGCAGAAGAGGACAAGTTAGAGCAAATCAAAAAATGGGCAGAGAAGTTAGACCGGCTAACAAGTACAGCA ACAAAAGATCCAGAAGGGTTTGTCGGACACCCTGTAAATGCATTCAAGTTAATGAAACGTCTGAACACCG AGTGGAGTGAGTTGGAGAATCTGATCCTCAAGGATATGTCAGATGGCTTCATCTCTAACCTGACCATTCA GAGGCAGTACTTCCCTAACGACGAAGACCAGGTTGGGGCTGCAAAAGCTTTGTTTCGTCTGCAAGACACC TACAACCTAGACACGAATACCATCTCGAAGGGCAATCTTCCAGGAGTGAAACACAAGTCTTTTCTAACAG CTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCACACAGAACTCTGGAT GGAGCAGGCTCTGATGCAGCTGGAGGAGGGAGAGATGTCTACTGTAGACAAAGTCTCGGTTCTAGATTAT TTGAGCTATGCAGTGTACCAGCAGGGTGACCTGGATAAGGCACTTCTGCTTACAAAGAAACTTCTTGAAC TAGATCCTGAACACCAGAGAGCCAATGGTAACTTAGTATATTTTGAGTATATAATGAGTAAAGAAAAAGA TGCCAATAAGTCTGCTTCGGGTGACCAGTCTGATCAGAAAACTACACCAAAGAAAAAGGGGATTGCTGTG GACTACCTGCCAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAAATGACTCCTCGGA GACAAAAAAGGCTGTTCTGCCGCTACCATGATGGAAACCGGAATCCTAAATTTATCCTGGCCCCAGCCAA GCAGGAGGATGAGTGGGACAAGCCTCGCATCATTCGTTTCCATGACATCATCTCAGATGCCGAGATTGAG ATCGTCAAAGATTTAGCAAAGCCCAGGCTGAGCCGAGCTACAGTACATGACCCTGAGACTGGGAAATTGA CCACAGCACAGTACAGAGTATCTAAGAGTGCTTGGCTGTCTGGCTATGAAGATCCTGTGGTGTCTCGAAT TAATATGAGAATACAAGATCTCACAGGACTGGATGTTTCCACGGCAGAGGAATTACAGGTAGCAAATTAT GGAGTTGGAGGACAGTATGAACCCCATTTTGACTTTGCCAGGAAAGACGAGCCGGATGCTTTTAGAGAGC TTGGGACAGGAAATAGGATTGCCACGTGGCTCTTCTACATGAGTGATGTGTCTGCTGGAGGCGCTACTGT TTTTCCTGAAGTGGGAGCCAGTGTTTGGCCCAAAAAAGGCACTGCTGTCTTCTGGTACAATCTGTTTGCC AGTGGAGAAGGAGATTACAGTACACGGCACGCAGCCTGTCCTGTGCTAGTGGGAAACAAATGGGTATCCA ACAAATGGCTCCATGAACGTGGACAGGAATTTCGAAGGCCGTGTACCCTGTCAGAATTGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206083 representing NM_172062
Red=Cloning site Green=Tags(s) MIWGVLMMGILLPQCSAHPGFFTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTA TKDPEGFVGHPVNAFKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDT YNLDTNTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALMQLEEGEMSTVDKVSVLDY LSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTTPKKKGIAV DYLPERQKYEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIE IVKDLAKPRLSRATVHDPETGKLTTAQYRVSKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEELQVANY GVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFA SGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172062 |
| ORF Size | 1602 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172062.2, NP_742059.2 |
| RefSeq Size | 2145 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 64475 |
| UniProt ID | P54001 |
| MW | 60.9 kDa |
| Gene Summary | catalyzes the hydroxylation of -X-Pro-Gly- triplets to form hydroxyproline [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206083 | P4ha1 (untagged ORF) - Rat procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I (P4ha1), (10 ug) |
CNY 6,840.00 |
|
| RR206083L3 | Lenti ORF clone of P4ha1 (Myc-DDK-tagged ORF) - Rat procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I (P4ha1), (10 ug) |
CNY 8,740.00 |
|
| RR206083L4 | Lenti ORF clone of P4ha1 (mGFP-tagged ORF) - Rat procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I (P4ha1), (10 ug) |
CNY 9,600.00 |
