Eaf2 (NM_172047) Rat Tagged ORF Clone

CAT#: RR204903

  • TrueORF®

Eaf2 (Myc-DDK-tagged ORF) - Rat ELL associated factor 2 (Eaf2), (10 ug)



  "NM_172047" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Traits
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR204903 representing NM_172047
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGGACCAGCGGGACTCGCATACCTCGACCGTCGCGAGCGGATTCTCAAATTAGGCGAAAGTTTCG
AGAAGCAGCCGCGCTGTGCTTTCCACACCGTGCGCTATGACTTCAAACCTGCTTCTGTTGATGCCTCTTG
TGAAGGAAATCTTGAGGTTGGCAAAGGTGAACAGGTGACAATAACTCTTCCAAATATAGAAGGTTCAACT
CCACCAGTCACAGTTTTCAAAGGTTCCAAGAGACCTTACTTAAAAGAGTGCATTTTGATTATTAACCATG
ATACTGGGGAATGTCGCCTAGAAAAACTCAGCAGCAACATCACTGTGAAAAAAACAAGAGGAGAAGGAAG
TAGCAAAATCCAGTGCAGACTAGAACAACAGCAACAACAAATGTGGAATCCACCCAGGACATCCAACCTT
GTACAGCATTCTCCATCAGAAGATAAGCTGTCCCCAACGTCTCTAATGGATGATATTGAAAGAGAGCTGA
AGGCGGAAGCTAGTCTTATGGACCAGATGAGTAGTTGTGATAGTTCATCCGATTCCAGAAGTTCTTCATC
TTCAAGTAGTGAGGACAGTTCTAGTGATTCCGAAGATGATGACCGATCCTCTCCTTCCGGTCCAAGGAGG
TACAGCTCAGAGCACCCCAGCGTATCTGCTGGGCCACAGTACAGGACTTCAGATGCTGACACTACTTGTA
ACAGACTTTACGACAACAGTGCCCTTCTGATGAGTACTTTACGAAGTGATCTGCAGCTGAGTGAGTCAGA
CAGCGACAGTGAGGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR204903 representing NM_172047
Red=Cloning site Green=Tags(s)

MNGPAGLAYLDRRERILKLGESFEKQPRCAFHTVRYDFKPASVDASCEGNLEVGKGEQVTITLPNIEGST
PPVTVFKGSKRPYLKECILIINHDTGECRLEKLSSNITVKKTRGEGSSKIQCRLEQQQQQMWNPPRTSNL
VQHSPSEDKLSPTSLMDDIERELKAEASLMDQMSSCDSSSDSRSSSSSSSEDSSSDSEDDDRSSPSGPRR
YSSEHPSVSAGPQYRTSDADTTCNRLYDNSALLMSTLRSDLQLSESDSDSED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_172047
ORF Size 786 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_172047.1, NP_742044.1
RefSeq Size 1132 bp
RefSeq ORF 789 bp
Locus ID 266787
UniProt ID Q811X5
MW 28.9 kDa
Gene Summary Acts as a transcriptional transactivator of ELL, ELL2 and TCEA1 elongation activities (By similarity). Potent inducer of apoptosis in prostatic and non-prostatic cell lines.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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