Grin2c (NM_012575) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GluN2C; NR2C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204373 representing NM_012575 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCTCTGGTTTCTCTGCAGGACCCTCCAGTGGACATGGGTGGGGCCCTAGGGCCCGCCCTGCTGC TCACTTCACTCCTTGGTGCTTGGGCAAGGCTGGGCGCAGGGCAGGGAGAACAGGCCGTGACTGTGGCGGT GGTGTTTGGCAGCTCTGGGCCACTGCAGACCCAGGCCCGGACTCGTCTTACCTCCCAGAACTTCCTGGAC CTGCCTCTGGAGATCCAGCCACTCACCGTAGGGGTCAACAATACCAATCCCAGCAGCATCCTCACCCAAA TCTGCGGGCTTCTGGGTGCCGCCCGTGTCCACGGCATCGTCTTTGAGGACAACGTGGACACCGAGGCCGT GGCCCAGCTACTGGACTTCGTCTCCTCCCAGACCCACGTGCCCATCCTCAGCATCAGCGGAGGCTCTGCT GTGGTCCTCACCCCCAAGGAGCCAGGCTCCGCCTTCCTACAGCTGGGCGTGTCCCTGGAGCAGCAGCTGC AGGTGCTGTTCAAGGTGCTGGAGGAATACGACTGGAGCGCGTTCGCGGTCATCACCAGCCTGCATCCGGG CCACGCGCTCTTCCTCGAAGGCGTGCGCGCCGTCGCGGACGCCAGCTACCTGAGCTGGCGGCTGCTGGAC GTGCTCACGCTGGAGCTAGGTCCGGGTGGGCCGCGAGCGCGCACTCAGCGCCTGCTACGCCAGGTCGACG CGCCGGTGCTGGTGGCTTACTGCTCCCGTGAAGAGGCCGAGGTGCTCTTCGCTGAGGCTGCACAGGCTGG CCTGGTGGGACCCGGGCACGTGTGGTTGGTACCTAACCTGGCGCTGGGAAGCACCGACGCACCCCCTGCA GCTTTCCCGGTGGGCCTCATCAGTGTGGTCACCGAGAGTTGGCGCCTTAGTCTACGCCAGAAGGTTCGCG ACGGAGTGGCCATTCTGGCCCTCGGTGCCCACAGCTACCGACGCCAGTACGGTACCCTGCCAGCCCCGGC TGGGGACTGCCGAAGCCACCCGGGACCCGTCAGCCCTGCTAGGGAGGCCTTCTACAGGCATCTGCTGAAT GTCACCTGGGAGGGCCGAGACTTCTCTTTCAGCCCTGGTGGGTACCTGGTTCGGCCCACCATGGTTGTGA TCGCCCTCAACCGGCACCGCCTCTGGGAGATGGTGGGACGGTGGGACCATGGAGTCCTGTACATGAAGTA TCCAGTATGGCCTCGCTACAGCACTTCTCTGCAGCCTGTGGTGGACAGCCGGCACCTGACAGTGGCCACA CTGGAAGAAAGGCCCTTTGTCATTGTGGAGAGCCCTGACCCTGGCACAGGTGGCTGTGTTCCCAACACCG TGCCCTGCCGCAGACAGAGCAACCACACCTTCAGCAGCGGGGATCTAACCCCCTACACCAAGCTCTGTTG TAAGGGCTTCTGCATCGACATCCTCAAAAAGCTGGCCAAGGTGGTCAAGTTCTCCTACGACTTGTACCTG GTGACCAACGGCAAACACGGCAAGAGGGTTCGTGGTGTGTGGAACGGCATGATCGGGGAGGTATACTACA AGCGGGCAGACATGGCCATCGGCTCCCTCACCATCAATGAGGAGCGCTCTGAGATTATAGATTTCTCTGT GCCTTTTGTGGAGACCGGCATCAGTGTTATGGTATCAAGGAGCAACGGCACCGTCTCCCCCTCAGCTTTT CTGGAACCCTACAGCCCTGCCGTGTGGGTGATGATGTTCGTGATGTGTCTCACGGTGGTTGCCATCACTG TCTTCATGTTCGAGTACTTCAGCCCTGTCAGCTACAACCAGAACCTCACCAAGGGCAAGAAACCTGGTGG ACCATCTTTCACCATTGGCAAGTCCGTGTGGTTGCTGTGGGCACTGGTCTTCAACAACTCTGTTCCCATC GAGAACCCCCGGGGTACCACCAGCAAGATCATGGTCCTGGTGTGGGCCTTCTTCGCTGTCATCTTCCTCG CTAGCTATACGGCCAATCTGGCGGCCTTCATGATCCAGGAGCAATACATCGACACTGTGTCGGGCCTTAG TGACAAGAAGTTTCAGCGGCCTCAAGACCAATACCCACCCTTCCGTTTTGGCACGGTACCTAACGGCAGC ACAGAGAGGAACATCCGTAGCAACTACCGTGACATGCACACCCACATGGTCAAGTTCAACCAGCGCTCGG TGGAGGACGCTCTCACAAGCCTGAAGATGGGGAAGCTGGACGCCTTCATCTATGATGCTGCTGTGCTCAA CTACATGGCGGGCAAGGACGAAGGCTGCAAGCTGGTCACCATTGGGTCTGGCAAAGTCTTTGCCACCACT GGCTATGGCATTGCCATGCAGAAAGACTCCCACTGGAAGCGGGCCATAGACCTGGCACTCCTGCAACTTC TGGGGGATGGAGAGACGCAGAAGCTGGAGACAGTGTGGCTCTCAGGGATCTGCCAGAACGAGAAGAATGA GGTGATGAGCAGCAAGCTGGACATTGACAACATGGCGGGGGTCTTCTACATGCTGTTGGTGGCCATGGGA CTGGCCCTTCTGGTCTTTGCCTGGGAGCACCTGGTCTACTGGAAACTTCGACACTCGGTGCCCAACTCAT CCCAGCTGGACTTCCTGCTGGCTTTCAGCAGGGGCATCTACAGCTGCTTCAACGGGGTACAGAGCCTTCC GAGTCCCGCACGGCCGCCCAGCCCGGATCTCACCGCAGACTCAGCCCAGGCCAATGTGCTGAAGATGCTG CAGGCGGCCCGAGACATGGTGAACACCGCGGACGTGAGCAGCTCTTTGGACCGCGCCACTCGCACCATCG AGAACTGGGGCAACAACCGCCGCGTGCCTGCTCCCACCGCCTCTGGCCCGCGGTCCTCCACCCCGGGTCC TCCGGGACAACCGAGCCCCAGCGGCTGGGGGCCTCCTGGTGGGGGCCGCACCCCGCTAGCGCGCAGGGCC CCGCAGCCTCCGGCTCGCCCCGCGACGTGCGGGCCACCTTTGCCCGATGTGTCCCGACCATCCTGCAGGC ACGCTTCGGACGCGCGGTGGCCGGTGCGAGTTGGGCATCAGGGACCGCACGTCTCGGCTTCCGAGCGGCG CGCGCTCCCGGAGCGCTCTCTGTTGCCCGCGCACTGCCACTACAGTTCCTTCCCTCGAGCGGAGAGATCC GGGCGCCCCTACCTCCCGTTATTCCCGGAGCCCCCGGAGCCCGACGACCTGCCTCTGCTCGGGCCGGAAC AGCTGGCTCGGCGGGAAGCAATGCTGCGCGCGGCCTGGGCCCGGGGCCCGCGCCCTCGCCACGCTTCCTT GCCCAGCTCGGTGGCAGAAGCTTTCACTCGATCCAACCCTCTGCCTGCCAGGTGTACCGGTCACGCCTGT GCCTGCCCGTGCCCCCAAAGCCGGCCATCCTGCAGGCACTTGGCTCAGGCACAGTCGCTGAGGCTGCCGT CCTACCCGGAGGCCTGTGTGGAGGGCGTACCAGCAGGGGTGGCCACCTGGCAGCCCAGACAGCACGTCTG CCTGCACGCCCATACCCGCCTGCCGTTCTGTTGGGGAACTGTCTGCCGTCACCCTCCACCCTGTACCAGC CACAGCCCCTGGCTCATTGGAACCTGGGAGCCTCCAGCACACAGAGTCAGGACCCTGGGGCTAGGCACAG GCTACAGGGACAGTGGGGTGCTGGAAGAGGTCAGCAGGGAAGCCTGTGGGACACAAGGTTTTCCAAGGTC CTGCACCTGGAGGCGGGTCTCCAGCCTGGAATCAGAAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204373 representing NM_012575 Red=Cloning site Green=Tags(s) MPALVSLQDPPVDMGGALGPALLLTSLLGAWARLGAGQGEQAVTVAVVFGSSGPLQTQARTRLTSQNFLD LPLEIQPLTVGVNNTNPSSILTQICGLLGAARVHGIVFEDNVDTEAVAQLLDFVSSQTHVPILSISGGSA VVLTPKEPGSAFLQLGVSLEQQLQVLFKVLEEYDWSAFAVITSLHPGHALFLEGVRAVADASYLSWRLLD VLTLELGPGGPRARTQRLLRQVDAPVLVAYCSREEAEVLFAEAAQAGLVGPGHVWLVPNLALGSTDAPPA AFPVGLISVVTESWRLSLRQKVRDGVAILALGAHSYRRQYGTLPAPAGDCRSHPGPVSPAREAFYRHLLN VTWEGRDFSFSPGGYLVRPTMVVIALNRHRLWEMVGRWDHGVLYMKYPVWPRYSTSLQPVVDSRHLTVAT LEERPFVIVESPDPGTGGCVPNTVPCRRQSNHTFSSGDLTPYTKLCCKGFCIDILKKLAKVVKFSYDLYL VTNGKHGKRVRGVWNGMIGEVYYKRADMAIGSLTINEERSEIIDFSVPFVETGISVMVSRSNGTVSPSAF LEPYSPAVWVMMFVMCLTVVAITVFMFEYFSPVSYNQNLTKGKKPGGPSFTIGKSVWLLWALVFNNSVPI ENPRGTTSKIMVLVWAFFAVIFLASYTANLAAFMIQEQYIDTVSGLSDKKFQRPQDQYPPFRFGTVPNGS TERNIRSNYRDMHTHMVKFNQRSVEDALTSLKMGKLDAFIYDAAVLNYMAGKDEGCKLVTIGSGKVFATT GYGIAMQKDSHWKRAIDLALLQLLGDGETQKLETVWLSGICQNEKNEVMSSKLDIDNMAGVFYMLLVAMG LALLVFAWEHLVYWKLRHSVPNSSQLDFLLAFSRGIYSCFNGVQSLPSPARPPSPDLTADSAQANVLKML QAARDMVNTADVSSSLDRATRTIENWGNNRRVPAPTASGPRSSTPGPPGQPSPSGWGPPGGGRTPLARRA PQPPARPATCGPPLPDVSRPSCRHASDARWPVRVGHQGPHVSASERRALPERSLLPAHCHYSSFPRAERS GRPYLPLFPEPPEPDDLPLLGPEQLARREAMLRAAWARGPRPRHASLPSSVAEAFTRSNPLPARCTGHAC ACPCPQSRPSCRHLAQAQSLRLPSYPEACVEGVPAGVATWQPRQHVCLHAHTRLPFCWGTVCRHPPPCTS HSPWLIGTWEPPAHRVRTLGLGTGYRDSGVLEEVSREACGTQGFPRSCTWRRVSSLESEV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012575
ORF Size	3750 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012575.3, NP_036707.3
RefSeq Size	4388 bp
RefSeq ORF	3753 bp
Locus ID	24411
UniProt ID	Q00961
MW	136.6 kDa
Gene Summary	subunit that in combination with NMDAR1 displays NMDA receptor activity; may contribute to NMDA receptor functional diversity [RGD, Feb 2006]

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