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Ucp2 (NM_019354) Rat Tagged ORF Clone
CAT＃: RR204365
- TrueORF®
Ucp2 (Myc-DDK-tagged ORF) - Rat uncoupling protein 2 (mitochondrial, proton carrier) (Ucp2), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_019354" in other vectors (3)
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CNY 3,600.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204365 representing NM_019354
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGGTTTCAAGGCCACCGATGTGCCCCCCACAGCCACCGTGAAGTTCCTGGGGGCTGGGACAGCAG CCTGTATTGCAGATCTCATCACTTTCCCTCTAGACACCGCCAAAGTCCGGCTGCAGATCCAAGGAGAGAG TCAAGGGCTAGCGCGCACCGCCGCCAGCGCCCAGTACCGCGGCGTGCTGGGCACCATCCTAACCATGGTG CGCACTGAGGGTCCGCGCAGCCTCTACAATGGGCTGGTCGCCGGCCTACAGCGCCAGATGAGCTTTGCCT CCGTCCGCATTGGCCTCTACGACTCTGTAAAGCAGTTCTACACCAAGGGCTCAGAGCATGCAGGCATCGG GAGCCGCCTCCTGGCAGGTAGCACCACAGGTGCCCTGGCTGTGGCTGTGGCCCAACCTACAGATGTGGTA AAGGTCCGCTTCCAGGCCCAGGCCCGGGCTGGCGGTGGTCGGAGATACCAGAGCACTGTCGAAGCCTACA AGACCATTGCACGAGAGGAAGGGATCCGGGGCCTCTGGAAAGGGACCTCTCCCAATGTTGCCCGAAATGC CATTGTCAACTGTACTGAGCTGGTGACCTATGACCTCATCAAAGATACTCTCCTGAAAGCCAACCTCATG ACAGACGACCTCCCTTGCCACTTCACTTCTGCCTTCGGGGCGGGCTTCTGCACCACCGTCATTGCCTCCC CCGTTGATGTGGTCAAGACGAGATATATGAACTCTGCCTTGGGCCAGTACCACAGCGCCGGCCACTGTGC CCTGACCATGCTCCGGAAGGAGGGGCCCCGAGCCTTCTACAAGGGGTTCATGCCTTCCTTCCTCCGCTTG GGATCCTGGAACGTAGTAATGTTTGTCACCTATGAGCAGCTCAAAAGGGCCCTGATGGCTGCCTATGAAT CCCGGGAGGCACCCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204365 representing NM_019354
Red=Cloning site Green=Tags(s) MVGFKATDVPPTATVKFLGAGTAACIADLITFPLDTAKVRLQIQGESQGLARTAASAQYRGVLGTILTMV RTEGPRSLYNGLVAGLQRQMSFASVRIGLYDSVKQFYTKGSEHAGIGSRLLAGSTTGALAVAVAQPTDVV KVRFQAQARAGGGRRYQSTVEAYKTIAREEGIRGLWKGTSPNVARNAIVNCTELVTYDLIKDTLLKANLM TDDLPCHFTSAFGAGFCTTVIASPVDVVKTRYMNSALGQYHSAGHCALTMLRKEGPRAFYKGFMPSFLRL GSWNVVMFVTYEQLKRALMAAYESREAPF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019354 |
| ORF Size | 927 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019354.3 |
| RefSeq Size | 930 bp |
| RefSeq ORF | 930 bp |
| Locus ID | 54315 |
| UniProt ID | P56500 |
| MW | 33.8 kDa |
| Gene Summary | catalyzes a proton leak across the inner mitochondrial membrane to uncouple oxidative phosphorylation; may reduce the concentration of reactive ozygen species in mitochondria [RGD, Feb 2006] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
An interplay between UCP2 and ROS protects cells from high-salt-induced injury through autophagy stimulation
,null,
Cell Death & Disease
,PubMed ID 34625529
[Ucp2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204365 | Ucp2 (untagged ORF) - Rat uncoupling protein 2 (mitochondrial, proton carrier) (Ucp2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 3,600.00 |
|
| RR204365L3 | Lenti ORF clone of Ucp2 (Myc-DDK-tagged ORF) - Rat uncoupling protein 2 (mitochondrial, proton carrier) (Ucp2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,080.00 |
|
| RR204365L4 | Lenti ORF clone of Ucp2 (mGFP-tagged ORF) - Rat uncoupling protein 2 (mitochondrial, proton carrier) (Ucp2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,650.00 |
