Sf3b3 (NM_001106187) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203604 representing NM_001106187 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCTGTACAACTTGACCTTACAGCGGGCCACTGGCATCAGCTTTGCCATCCATGGCAACTTCTCTG GAACCAAACAACAGGAAATTGTCGTTTCCCGTGGGAAGATTTTGGAGTTACTTCGCCCAGATCCTAACAC TGGCAAAGTGCATACCCTTCTCACTGTTGAAGTTTTTGGTGTTATCCGGTCACTTATGGCATTTAGGCTA ACAGGTGGCACCAAAGACTACATTGTAGTTGGCAGTGACTCAGGCCGAATTGTTATCTTGGAATACCAGC CATCAAAGAATATGTTTGAGAAAATTCACCAAGAAACTTTCGGCAAAAGTGGATGTCGCCGTATTGTTCC TGGCCAGTTCTTAGCCGTGGATCCCAAAGGAAGAGCTGTTATGATTAGTGCGATTGAGAAGCAGAAATTG GTGTATATCTTGAACAGAGACGCTGCAGCCCGACTTACCATTTCATCTCCCCTGGAGGCCCACAAAGCAA ACACTCTAGTGTACCATGTGGTTGGAGTAGATGTGGGATTTGAAAATCCTATGTTTGCTTGTCTGGAAAT GGATTATGAGGAAGCAGACAATGATCCAACAGGGGAAGCAGCAGCCAATACCCAGCAGACACTTACTTTC TATGAATTGGACCTTGGTTTAAATCACGTGGTTCGGAAATACAGTGAACCGTTGGAGGAACATGGCAACT TCCTTATTACAGTTCCTGGAGGGTCAGATGGTCCAAGTGGAGTGCTGATCTGCTCTGAGAATTATATCAC CTACAAGAACTTTGGTGACCAGCCAGACATCCGATGTCCAATTCCCAGGAGACGGAATGACCTGGATGAC CCTGAAAGAGGAATGATTTTTGTTTGCTCTGCCACCCATAAGACCAAGTCCATGTTCTTCTTTTTGGCCC AAACGGAGCAGGGAGATATCTTTAAGATCACTTTGGAGACAGATGAAGATATGGTAACTGAGATCCGCCT CAAGTATTTTGATACTGTTCCTGTTGCTGCTGCTATGTGTGTGCTTAAAACAGGCTTCCTTTTTGTGGCA TCAGAATTTGGAAATCATTACTTGTATCAAATTGCCCATCTTGGAGATGATGATGAAGAGCCTGAGTTTT CATCTGCCATGCCTTTAGAAGAAGGAGACACATTCTTCTTTCAGCCGAGACCACTCAAAAATCTCGTGCT GGTGGATGAACTGGACAGCCTCTCTCCTATTCTCTTTTGCCAGATAGCTGATTTGGCCAATGAAGACACT CCACAGCTGTATGTAGCCTGTGGTAGGGGACCCCGGTCGTCTCTGAGAGTCTTAAGACATGGACTTGAGG TGTCGGAAATGGCTGTTTCTGAGCTCCCTGGTAACCCCAATGCTGTCTGGACTGTCCGTCGGCACATTGA AGATGAGTTTGATGCCTACATCATTGTGTCTTTCGTGAATGCCACGCTGGTGCTGTCCATCGGAGAGACT GTGGAAGAAGTGACAGACTCCGGGTTCTTGGGTACCACTCCAACCTTGTCCTGTTCCTTATTGGGAGATG ACGCGTTGGTGCAGGTGTATCCAGATGGCATTCGACACATCCGAGCAGACAAGAGAGTCAATGAGTGGAA GACTCCTGGCAAGAAGACAATTGTGAAGTGTGCAGTGAACCAGCGACAAGTGGTGATCGCCTTGACTGGG GGAGAGCTGGTCTATTTCGAGATGGATCCTTCAGGACAGTTGAATGAGTACACGGAGCGGAAAGAAATGT CAGCAGATGTGGTGTGCATGAGTCTGGCCAACGTACCCCCTGGAGAACAGCGGTCTCGGTTCCTGGCTGT GGGGCTGGTGGACAACACTGTCAGAATCATCTCCCTTGACCCATCAGACTGTTTGCAGCCTCTGAGTATG CAGGCTCTCCCAGCCCAGCCTGAGTCTCTGTGTATTGTGGAAATGGGTGGGACCGAGAAACAAGATGAGC TGGGTGAGAGGGGCTCTATCGGCTTCCTGTACCTGAACATTGGACTCCAGAACGGTGTGCTGCTGAGGAC TGTCCTAGACCCTGTTACTGGGGACCTGTCTGACACCCGCACTAGGTATCTGGGGTCCCGTCCTGTGAAG CTCTTTCGGGTTCGGATGCAAGGCCAGGAGGCTGTTCTGGCTATGTCTAGTCGCTCATGGCTGAGCTACT CTTACCAGTCTCGATTCCATCTCACCCCTCTGTCTTATGAGACCCTGGAATTTGCATCGGGCTTTGCCTC TGAACAGTGTCCTGAAGGCATCGTTGCCATCTCCACCAACACTTTGAGGATTTTGGCACTAGAGAAGCTT GGCGCTGTCTTCAATCAAGTGGCCTTTCCTTTGCAGTACACACCCAGGAAGTTTGTCATCCACCCTGAGA GTAACAACCTTATCATCATAGAGACGGATCACAATGCCTACACTGAGGCTACAAAAGCTCAGAGAAAGCA GCAGATGGCTGAGGAAATGGTAGAAGCAGCAGGGGAGGATGAACGGGAGCTGGCTGCAGAGATGGCAGCC GCGTTCCTCAATGAAAACCTCCCAGAATCCATTTTCGGAGCTCCCAAGGCTGGCAATGGGCAGTGGGCCT CTGTGATTCGGGTAATGAACCCTATCCAGGGGAATACACTAGACCTTGTCCAGCTGGAGCAGAATGAGGC AGCCTTTAGTGTGGCAGTGTGTAGGTTCTCCAACACTGGTGAAGACTGGTATGTGCTGGTTGGTGTTGCT AAGGACTTGATCCTGAGCCCTCGGTCTGTGGCAGGGGGCTTTGTGTATACATACAAGCTTGTGAACAATG GGGAAAAGTTGGAGTTTTTGCACAAGACTCCAGTGGAAGAAGTCCCTGCTGCCATTGCCCCATTCCAGGG GAGGGTACTGATTGGTGTGGGGAAACTTTTACGAGTCTATGACCTGGGGAAGAAGAAGTTACTTCGAAAG TGTGAGAATAAGCATATTGCCAACTACATATCAGGGATCCAGACAATTGGTCACAGGGTAATCGTGTCTG ATGTCCAGGAAAGTTTCATCTGGGTTCGCTACAAGCGTAATGAGAACCAGCTCATTATCTTTGCTGATGA CACCTATCCCAGATGGGTCACCACTGCCAGCCTTCTCGACTATGATACTGTGGCTGGGGCAGACAAGTTT GGCAACATTTGTGTGGTGAGGCTCCCACCTAACACCAACGATGAGGTGGATGAAGATCCCACTGGAAACA AAGCCCTGTGGGACCGGGGCTTGCTCAATGGGGCTTCACAGAAGGCAGAGGTCATCATGAACTACCACGT TGGGGAGACAGTGCTGTCGTTGCAGAAGACCACACTGATCCCTGGAGGCTCAGAATCACTTGTCTATACC ACTTTATCTGGAGGAATTGGCATCCTTGTCCCTTTCACGTCTCATGAGGACCACGACTTTTTCCAGCATG TTGAGATGCACCTGCGGTCTGAGCATCCTCCTCTCTGTGGACGGGACCACCTCAGCTTTCGTTCTTATTA TTTCCCTGTTAAGAATGTGATTGATGGAGACCTTTGTGAGCAGTTCAACTCCATGGAGCCCAACAAGCAG AAAAATGTCTCTGAAGAGCTGGACCGAACCCCACCTGAAGTGTCCAAGAAGCTTGAGGATATTCGGACCC GCTATGCTTTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203604 representing NM_001106187 Red=Cloning site Green=Tags(s) MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPDPNTGKVHTLLTVEVFGVIRSLMAFRL TGGTKDYIVVGSDSGRIVILEYQPSKNMFEKIHQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKL VYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTF YELDLGLNHVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDD PERGMIFVCSATHKTKSMFFFLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVPVAAAMCVLKTGFLFVA SEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDELDSLSPILFCQIADLANEDT PQLYVACGRGPRSSLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGET VEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTG GELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSM QALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVK LFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKL GAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAA AFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVA KDLILSPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRK CENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKF GNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYT TLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQ KNVSEELDRTPPEVSKKLEDIRTRYAF SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106187
ORF Size	3651 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106187.2, NP_001099657.2
RefSeq Size	4524 bp
RefSeq ORF	3654 bp
Locus ID	292019
MW	135.6 kDa

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