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Sema3a (NM_017310) Rat Tagged ORF Clone
CAT＃: RR201384
- TrueORF®
Sema3a (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), (10 ug)
"NM_017310" in other vectors (3)
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CNY 9,980.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201384 representing NM_017310
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGGTTCACTGGGATTGCCTGTCTTTTCTGGGGTATATTACTTACAGCAAGAGCAAACTATGCAA ACGGAAAGAACAATGTGCCAAGACTGAAATTATCGTACAAAGAAATGTTGGAATCCAACAATGTGATCAC TTTCAATGGCTTGGCCAACAGCTCCAGTTATCACACTTTTCTTCTGGATGAGGAACGGAGTAGACTGTAT GTTGGAGCAAAGGATCATATATTTTCATTCAACTTGGTGAATATTAAAGATTTTCAAAAGATTGTGTGGC CAGTATCTTATACCAGGAGAGATGAATGCAAGTGGGCTGGAAAAGATATCCTGAAAGAATGTGCAAATTT CATCAAGGTCCTCAAGGCTTATAATCAGACTCACTTGTACGCCTGTGGAACGGGGGCTTTCCATCCAATT TGCACCTATATTGAAGTTGGACATCATCCTGAGGACAACATTTTTAAACTGCAGGACTCACATTTTGAAA ACGGTCGTGGGAAGAGTCCTTATGACCCCAAACTTCTGACTGCCTCTCTTCTAATAGATGGTGAGTTATA CTCTGGAACTGCTGCGGATTTCATGGGACGAGACTTTGCTATCTTCAGAACATTGGGGCACCATCATCCC ATCAGGACCGAGCAGCACGACTCCCGGTGGCTCAATGATCCTAGATTCATCAGTGCCCATCTCATCCCAG AGAGTGATAACCCTGAAGATGACAAAGTATATTTTTTCTTCCGAGAAAATGCAATAGATGGAGAACATTC TGGAAAAGCCACTCATGCTAGAATAGGTCAGATATGCAAGAATGACTTTGGAGGACACAGAAGTCTTGTG AATAAATGGACAACTTTCCTGAAAGCACGCCTGATTTGCTCTGTGCCAGGTCCCAATGGCATTGACACAC ATTTTGATGAATTACAGGATGTATTCTTAATGAACTCTAAAGATCCTAAAAATCCAATTGTCTATGGAGT GTTCACAACATCAAGCAACATCTTTAAGGGCTCTGCTGTGTGTATGTACAGTATGAGTGATGTAAGAAGG GTGTTCCTTGGTCCATATGCTCACAGAGATGGTCCCAACTATCAGTGGGTACCTTACCAAGGAAGAGTCC CTTATCCACGACCAGGAACTTGTCCCAGTAAAACATTTGGTGGATTTGACTCCACAAAGGACCTTCCTGA TGATGTCATAACCTTTGCAAGAAGCCATCCAGCCATGTACAACCCAGTGTTTCCTATTAATAATCGCCCA ATAATGATCAAAACAGATGTAAATTATCAGTTCACACAAATTGTTGTAGACCGGGTGGATGCAGAAGATG GCCAGTATGATGTTATGTTCATCGGAACGGATGTTGGAACTGTCCTTAAAGTGGTTTCAGTCCCCAAGGA GACTTGGCATGACCTAGAAGAAGTTCTTCTGGAAGAAATGACAGTCTTCCGGGAACCAACAACTATTTCA GCAATGGAGCTTTCTACTAAACAGCAACAACTGTACATTGGCTCAACTGCTGGAGTAGCACAGCTTCCTC TACACCGTTGTGACATTTATGGCAAAGCCTGTGCGGAATGCTGCCTTGCTCGGGACCCTTATTGTGCCTG GGATGGGTCATCATGCTCACGCTATTTTCCTACTGCAAAGAGGCGCACAAGACGACAGGATATAAGGAAT GGAGACCCACTGACTCACTGCTCCGACTTGCAGCATCATGATAATCATCATGGGCACAGTCTTGAAGAGA GAATCATCTATGGAGTAGAAAATAGTAGCACATTCTTGGAATGCAGTCCGAAGTCACAGAGAGCCTTGGT ATATTGGCAATTTCAGAGGCGAAATGAAGATCGAAAGGAGGAGATCAGAGTGGGTGATCATATCATCAGG ACAGAACAGGGGCTCCTGCTTCGTAGTCTGCAGAAGAAGGATTCAGGCAATTACCTGTGTCACGCCGTGG AGCACGGTTTCATGCAAACTCTTCTTAAGGTGACCCTGGAAGTCATCGATACAGAACATTTGGAGGAACT TCTTCATAAAGATGACGATGGAGATGGCTCTAAGACCAAAGAAATGTCCAGTAGCATGACGCCCAGCCAG AAGGTCTGGTACAGAGACTTCATGCAGCTCATCAACCACCCCAACCTGAACACAATGGATGAGTTCTGTG AACAAGTGTGGAAAAGGGACCGAAAACAACGCCGACAAAGGCCGGGGCACTCTCAAGGGAGCAGCAACAA GTGGAAGCACATGCAGGAGAGCAAGAAAGGTAGAAACAGGAGGACCCACGAGTTTGAGAGGGCACCCAGG AGTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201384 representing NM_017310
Red=Cloning site Green=Tags(s) MGWFTGIACLFWGILLTARANYANGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLY VGAKDHIFSFNLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPI CTYIEVGHHPEDNIFKLQDSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHP IRTEQHDSRWLNDPRFISAHLIPESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLV NKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNSKDPKNPIVYGVFTTSSNIFKGSAVCMYSMSDVRR VFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPINNRP IMIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSVPKETWHDLEEVLLEEMTVFREPTTIS AMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSSCSRYFPTAKRRTRRQDIRN GDPLTHCSDLQHHDNHHGHSLEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEDRKEEIRVGDHIIR TEQGLLLRSLQKKDSGNYLCHAVEHGFMQTLLKVTLEVIDTEHLEELLHKDDDGDGSKTKEMSSSMTPSQ KVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHSQGSSNKWKHMQESKKGRNRRTHEFERAPR SV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017310 |
| ORF Size | 2316 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017310.1, NP_059006.1 |
| RefSeq Size | 2319 bp |
| RefSeq ORF | 2319 bp |
| Locus ID | 29751 |
| UniProt ID | Q63548 |
| MW | 88.8 kDa |
| Gene Summary | induces neuronal cell death; induces apoptosis of retinal ganglion cells in response to injury [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201384 | Sema3a (untagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), (10 ug) |
CNY 8,488.00 |
|
| RR201384L3 | Lenti ORF clone of Sema3a (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), (10 ug) |
CNY 11,780.00 |
|
| RR201384L4 | Lenti ORF clone of Sema3a (mGFP-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), (10 ug) |
CNY 12,920.00 |
