Atp7b (NM_012511) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hts; PINA; Wd
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206811 representing NM_012511 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGAACAGGAGAGAAAGGTCACAGCCAAAGAGGCCAGTCGGAAAATCTTATCTAAACTTGCTTTGC CCACACGACCGTGGGGACAATCAATGAAGCAGAGCTTCGCCTTCGATAATGTTGGCTATGAAGGGGGCCT GGACAGCACCTGCTCATCCTCAACTACCACCGGTGTGGTTAGCATCCTGGGCATGACTTGTCATTCTTGC GTCAAGTCCATCGAGGACAGGATCTCCAGTCTGAAAGGCATTGTGAGCATCAAGGTTTCTCTGGAGCAGG GCAGCGCCACTGTCAAATATGTACCGTCAGTCTTGAACCTGCAGCAGATTTGCCTTCAGATTGAGGACAT GGGCTTTGAGGCCAGCGCTGCAGAAGGAAAGGCTGCCTCCTGGCCTTCCAGGTCTTCCCCAGCCCAGGAG GCAGTGGTCAAGCTCCGGGTAGAGGGCATGACCTGTCAGTCCTGTGTCAGCTCCATCGAAGGCAAGATCC GGAAGCTGCAAGGGGTTGTGAGAGTCAAAGTCTCCCTAAGCAACCAAGAGGCAGTCATTACATATCAGCC TTACCTCATTCAACCCGAAGACCTCAGGGACCACATCTGCGACATGGGATTCGAAGCTGCCATCAAGAAC AGAACAGCTCCCTTAAGGCTGGGACCAATTGATATCAACAAGTTAGAAAGCACTAACCTAAAGAGAGCAG CAGTCCCTCCTATCCAGAATTCCAATCATTTGGAGACCCCGGGGCACCAGCAGAACCACCTGGCCACCCT CCCACTAAGAATAGACGGGATGCACTGTAAATCATGTGTTTTGAATATCGAAGGAAATATAGGCCAACTT CCAGGGGTTCAAAATATTCATGTGTCCTTGGAGAACAAAACCGCCCAAGTACAGTATGACTCTTCTTGTA TCACCCCCTTGTTCCTACAGACAGCCATCGAGGCACTACCACCTGGGTACTTTAAAGTATCCCTTCCCGA TGGCCTAGAGAAGGAGAGTGGATCTTCCAGTGTCCCCTCCCTTGGCTCCTCCCAGAGACAGCAGGAGCCA GGCCCATGCAGGACTGCGGTACTCACCATCACTGGCATTCCCCGTGACTCGTCTGTTCAGCCCATGGAAG ACATGCTGTCCCAGATGAAGGGTGTGCAGCAAATAGACATCTCTTTGGCAGAGGGGACTGGAGCAGTTCT TTACGATCCCTCAGTAGTTAGCTCGGATGAACTCCGGACGGCTGTAGAAGACATGGGCTTTGAGGTGTCA GTGAATCCCGAAAACATTACTACTAACCGTGTCAGCTCTGGGAATTCTGTGCCACAAGCCGTGGGTGATT CACCAGGGTCTGTGCAAAATATGGCTTCTGACACTAGAGGACTCCTCACACACCAAGGCCCTGGCTACTT GTCAGACAGCCCACCATCCCCTGGAGGAACAGCATCACAGAAGTGCTTTGTACAGATCAAAGGCATGACC TGTGCGTCCTGTGTGTCTAACATAGAAAGGAGTCTGCAGAGACATGCCGGTATTCTCTCCGTGTTGGTCG CCTTGATGTCGGGAAAGGCAGAGGTCAAGTATGACCCAGAGGTCATCCAGTCTCCCAGGATAGCTCAGCT CATCGAGGACCTGGGCTTCGAAGCAGCAATCATGGAGGACAACACAGTCTCTGAAGGTGACATCGAACTG ATTATCACAGGGATGACCTGCGCTTCCTGTGTTCACAACATAGAATCTAAGCTCACAAGGACAAATGGCA TCACTTACGCCTCTGTGGCCCTCGCCACCAGCAAAGCCCATGTGAAGTTTGACCCTGAAATCATTGGTCC ACGTGACATCATCAAGGTCATCGAGGAAATCGGCTTTCATGCTTCCCTGGCCCACAGAAACCCCAACGCT CATCACTTGGACCACAAGACGGAAATAAAACAGTGGAAGAAATCTTTCCTGTGCAGCCTGGTGTTTGGCA TCCCCGTCATGGGCTTGATGATCTACATGCTAATCCCCAGCAGTAAGCCCCACGAGACCATGGTCCTGGA CCACAACATCATTCCAGGACTGTCCGTTCTAAACCTCATTTTCTTCATCTTGTGTACCTTCGTCCAATTC CTGGGTGGGTGGTACTTCTATGTCCAAGCCTACAAATCGCTGAGACACAAGTCAGCCAACATGGATGTGC TCATCGTACTCGCCACGACCATTGCCTATGCCTACTCCCTGGTCATCCTGGTGGTTGCCATAGCTGAAAA GGCGGAGAAGAGCCCAGTGACCTTCTTTGACACACCCCCCATGCTCTTCGTCTTCATCGCCCTGGGACGG TGGCTGGAGCACGTGGCAAAGAGCAAAACTTCAGAAGCCCTCGCAAAACTCATGTCACTCCAAGCCACAG AAGCCACAGTTGTGACCCTGGGAGAGGACAACTTAATCCTCAGAGAGGAGCAAGTGCCCATGGAGCTGGT GCAGCGAGGTGACATCATCAAGGTTGTCCCTGGGGGCAAGTTCCCAGTGGACGGGAAAGTCCTGGAAGGC AACACCATGGCAGATGAGTCCCTCATCACAGGAGAGGCCATGCCTGTCACCAAGAAACCCGGGAGCATAG TGATTGCTGGCTCTATAAATGCTCATGGCTCTGTGCTCATTAAAGCTACCCATGTGGGCAATGACACTAC TTTGGCTCAGATTGTCAAGTTGGTGGAAGAGGCCCAGATGTCAAAGGCTCCCATTCAGCAGCTGGCTGAC CGGTTCAGTGGATATTTCGTCCCATTTATCATCATTATTTCAACCTTAACATTGGTGGTGTGGATCATCA TCGGCTTTGTCGATTTTGGTATTGTTCAGAAGTACTTTCCTAGCCCTAGCAAGCATATCTCACAGACAGA GGTGATCATCCGCTTTGCCTTCCAGACGTCCATCACCGTCCTGTGCATCGCCTGCCCCTGCTCCCTCGGG CTGGCCACACCCACAGCAGTTATGGTGGGCACTGGGGTGGCTGCCCAGAACGGCGTCCTAATCAAGGGAG GGAAGCCTCTGGAGATGGCACACAAGATAAAGACCGTTATGTTTGACAAAACGGGCACCATTACCCACGG GGTCCCCAGAGTCATGCGGTTTCTGCTGCTTGTGGACGTGGCTACCCTATCCCTCAGGAAGGTTCTGGCT GTGGTGGGCACCGCAGAGGCCAGCAGTGAGCACCCCTTAGGCGTGGCCGTCACTAAATACTGCAAAGAGG AACTCGGGACGGAGACCCTGGGGTACAGCACGGACTTCCAGGCAGTGCCAGGGTGTGGAATTAGCTGCAA AGTTAGCAACGTGGAAAGTATCCTGGCTCACAGAGGTCCAACCGCTCACCCGATTGGGGTTGGCAACCCT CCCATAGGAGAAGGTACAGGTCCCCAGACTTTCTCTGTGCTGATTGGAAACCGGGAATGGATGAGGCGCA ATGGTTTAACCATCTCCAGTGACATCAGTGACGCCATGACAGATCATGAAATGAAAGGACAGACGGCCAT CCTGGTGGCCATTGATGGTGTGCTGTGCGGGATGATCGCCATTGCAGATGCTGTTAAACCAGAGGCTGCC CTGGCTATCTACACCCTGAAAAGCATGGGCGTGGATGTGGCTCTGATCACAGGGGACAACCGGAAGACAG CCAGAGCCATTGCCACTCAGGTTGGCATCAACAAAGTCTTTGCTGAGGTACTGCCTTCTCACAAGGTGGC CAAGGTCCAGGAGCTTCAGAACAAAGGGAAAAAAGTCGCCATGGTGGGAGACGGGGTGAACGACTCCCCA GCCTTGGCCCAGGCTGACGTGGGCATTGCTATTGGGACTGGGACAGATGTCGCCATCGAAGCAGCCGACG TGGTCCTTATAAGAAATGACTTACTGGACGTGGTGGCCAGCATTCATCTCTCCAAGAGGACCGTCCGGAG GATCCGGGTCAATCTGGTGCTGGCGTTGATTTATAACATGGTTGGGATACCCATTGCTGCAGGTGTCTTC ATGCCCATTGGCATCGTGCTGCAGCCATGGATGGGCTCAGCGGCCATGGCCGCCTCCTCTGTGTCCGTGG TGCTCTCCTCTCTTCAGCTCAAGTGCTACAGAAAGCCCGACCTAGAGAGATATGAGGCACAGGCCCATGG ACGCATGAAGCCTCTGAGTGCATCCCAAGTCAGCGTGCACGTTGGCATGGATGACCGGCGGCGGGATTCT CCCAGGGCCACACCCTGGGACCAGGTCAGCTACGTGAGCCAAGTCTCTCTGTCTTCCCTGACGTCAGACA GATTGTCTCGGCATGGCGGTATGGCAGAGGATGGTGGAGACAAATGGTCCCTGCTCCTGAGTGACAGGGA TGAAGAGCAGTGCATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012511
Insert Size	4359 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012511.2, NP_036643.2
RefSeq Size	5506 bp
RefSeq ORF	4359 bp
Locus ID	24218
Gene Summary	plays a role in copper ion transport; partial gene deletion in the Long-Evans Cinnamon (LEC) strain is a model for Wilson disease [RGD, Feb 2006]

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