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DAP Kinase 1 (DAPK1) (NM_001288731) Human Tagged ORF Clone
CAT＃: RG240141
- TrueORF®
DAPK1 (tGFP-tagged) - Human death-associated protein kinase 1 (DAPK1), transcript variant 4
ORF Plasmid: DDK
"NM_001288731" in other vectors (2)
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CNY 6,080.00
货期*
2周
规格
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Tag | TurboGFP |
| Synonyms | DAPK; ROCO3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG240141 representing NM_001288731.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCGTGTTCAGGCAGGAAAACGTGGATGATTACTACGACACCGGCGAGGAACTTGGCAGTGGACAG TTTGCGGTTGTGAAGAAATGCCGTGAGAAAAGCACCGGCCTCCAGTATGCCGCCAAATTCATCAAGAAA AGGAGGACTAAGTCCAGCCGGCGGGGTGTGAGCCGCGAGGACATCGAGCGGGAGGTCAGCATCCTGAAG GAGATCCAGCACCCCAATGTCATCACCCTGCACGAGGTCTATGAGAACAAGACGGACGTCATCCTGATC TTGGAACTCGTTGCAGGTGGCGAGCTGTTTGACTTCTTAGCTGAAAAGGAATCTTTAACTGAAGAGGAA GCAACTGAATTTCTCAAACAAATTCTTAATGGTGTTTACTACCTGCACTCCCTTCAAATCGCCCACTTT GATCTTAAGCCTGAGAACATAATGCTTTTGGATAGAAATGTCCCCAAACCTCGGATCAAGATCATTGAC TTTGGGTTGGCCCATAAAATTGACTTTGGAAATGAATTTAAAAACATATTTGGGACTCCAGAGTTTGTC GCTCCTGAGATAGTCAACTATGAACCTCTTGGTCTTGAGGCAGATATGTGGAGTATCGGGGTAATAACC TATATCCTCCTAAGTGGGGCCTCCCCATTTCTTGGAGACACTAAGCAAGAAACGTTAGCAAATGTATCC GCTGTCAACTACGAATTTGAGGATGAATACTTCAGTAATACCAGTGCCCTAGCCAAAGATTTCATAAGA AGACTTCTGGTCAAGGATCCAAAGAAGAGAATGACAATTCAAGATAGTTTGCAGCATCCCTGGATCAAG CCTAAAGATACACAACAGGCACTTAGTAGAAAAGCATCAGCAGTAAACATGGAGAAATTCAAGAAGTTT GCAGCCCGGAAAAAATGGAAACAATCCGTTCGCTTGATATCACTGTGCCAAAGATTATCCAGGTCATTC CTGTCCAGAAGTAACATGAGTGTTGCCAGAAGCGATGATACTCTGGATGAGGAAGACTCCTTTGTGATG AAAGCCATCATCCATGCCATCAACGATGACAATGTCCCAGGCCTGCAGCACCTTCTGGGCTCATTATCC AACTATGATGTTAACCAACCCAACAAGCACGGGACACCTCCATTACTCATTGCTGCTGGCTGTGGGAAT ATTCAAATACTACAGTTGCTCATTAAAAGAGGCTCGAGAATCGATGTCCAGGATAAGGGCGGGTCCAAT GCCGTCTACTGGGCTGCTCGGCATGGCCACGTCGATACCTTGAAATTTCTCAGTGAGAACAAATGCCCT TTGGATGTGAAAGACAAGTCTGGAGAGATGGCCCTCCACGTGGCAGCTCGCTATGGCCATGCTGACGTG GCTCAGTTACTGTGCAGCTTCGGCTCAAATCCCAATATCCAGGACAAGGAAGAAGAAACCCCCCTGCAC TGTGCTGCTTGGCACGGCTATTACTCTGTGGCCAAAGCCCTTTGTGAAGCCGGCTGTAACGTGAACATC AAGAACCGAGAAGGAGAGACGCCCCTCCTGACAGCCTCTGCCAGGGGCTACCACGACATCGTGGAGTGT CTGGCCGAACATGGAGCCGACCTTAATGCTTGCGACAAGGACGGACACATTGCCCTTCATCTGGCTGTA AGACGGTGTCAGATGGAGGTAATCAAGACTCTCCTCAGCCAAGGGTGTTTCGTCGATTATCAAGACAGG CACGGCAATACTCCCCTCCATGTGGCATGTAAAGATGGCAACATGCCTATCGTGGTGGCCCTCTGTGAA GCAAACTGCAATTTGGACATCTCCAACAAGTATGGGCGAACGCCTCTGCACCTTGCGGCCAACAACGGA ATCCTAGACGTGGTCCGGTATCTCTGTCTGATGGGAGCCAGCGTTGAGGCGCTGACCACGGACGGAAAG ACGGCAGAAGATCTTGCTAGATCGGAACAGCACGAGCACGTAGCAGGTCTCCTTGCAAGACTTCGAAAG GATACGCACCGAGGACTCTTCATCCAGCAGCTCCGACCCACACAGAACCTGCAGCCAAGAATTAAGCTC AAGCTGTTTGGCCACTCGGGATCCGGGAAAACCACCCTTGTAGAATCTCTCAAGTGTGGGCTGCTGAGG AGCTTTTTCAGAAGGCGTCGGCCCAGACTGTCTTCCACCAACTCCAGCAGGTTCCCACCTTCACCCCTG GCTTCTAAGCCCACAGTCTCAGTGAGCATCAACAACCTGTACCCAGGCTGCGAGAACGTGAGTGTGAGG AGCCGCAGCATGATGTTCGAGCCGGGTCTTACCAAAGGGATGCTGGAGGTGTTTGTGGCCCCGACCCAC CACCCGCACTGCTCGGCCGATGACCAGTCCACCAAGGCCATCGACATCCAGAACGCTTATTTGAATGGA GTTGGCGATTTCAGCGTGTGGGAGTTCTCTGGAAATCCTGTGTATTTCTGCTGTTATGACTATTTTGCT GCAAATGATCCCACGTCAATCCATGTTGTTGTCTTTAGTCTAGAAGAGCCCTATGAGATCCAGCTGAAC CAAGTGATTTTCTGGCTCAGTTTCCTGAAGTCCCTTGTCCCAGTTGAAGAACCCATAGCCTTCGGTGGC AAGCTGAAGAACCCACTCCAAGTTGTCCTGGTGGCCACCCACGCTGACATCATGAATGTTCCTCGACCG GCTGGAGGCGAGTTTGGATATGACAAAGACACATCGTTGCTGAAAGAGATTAGGAACAGGTTTGGAAAT GATCTTCACATTTCAAATAAGCTGTTTGTTCTGGATGCTGGGGCTTCTGGGTCAAAGGACATGAAGGTA CTTCGAAATCATCTGCAAGAAATACGAAGCCAGATTGTTTCGGTCTGTCCTCCCATGACTCACCTGTGT GAGAAAATCATCTCCACGCTGCCTTCCTGGAGGAAGCTCAATGGACCCAACCAGCTGATGTCGCTGCAG CAGTTTGTGTACGACGTGCAGGACCAGCTGAACCCCCTGGCCAGCGAGGAGGACCTCAGGCGCATTGCT CAGCAGCTCCACAGCACAGGCGAGATCAACATCATGCAAAGTGAAACAGTTCAGGACGTGCTGCTCCTG GACCCCCGCTGGCTCTGCACAAACGTCCTGGGGAAGTTGCTGTCCGTGGAGACCCCACGGGCGCTGCAC CACTACCGGGGCCGCTACACCGTGGAGGACATCCAGCGCCTGGTGCCCGACAGCGACGTGGAGGAGCTG CTGCAGATCCTCGATGCCATGGACATCTGCGCCCGGGACCTGAGCAGCGGGACCATGGTGGACGTCCCA GCCCTGATCAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAGGACGAGGTGATGGTGTATGGT GGCGTGCGCATCGTGCCCGTGGAACACCTCACCCCCTTCCCATGTGGCATCTTTCACAAGGTCCAGGTG AACCTGTGCCGGTGGATCCACCAGCAAAGCACAGAGGGCGACGCGGACATCCGCCTGTGGGTGAATGGC TGCAAGCTGGCCAACCGTGGGGCCGAGCTGCTGGTGCTGCTGGTCAACCACGGCCAGGGCATTGAGGTC CAGGTCCGCGGCCTGGAGACGGAGAAGATCAAGTGCTGCCTGCTGCTGGACTCGGTGTGCAGCACCATT GAGAACGTCATGGCCACCACGCTGCCAGGGCTCCTGACCGTGAAGCATTACCTGAGCCCCCAGCAGCTG CGGGAGCACCATGAGCCCGTCATGATCTACCAGCCACGGGACTTCTTCCGGGCACAGACTCTGAAGGAA ACCTCACTGACCAACACCATGGGGGGGTACAAGGAAAGCTTCAGCAGCATCATGTGCTTCGGGTGTCAC GACGTCTACTCACAGGCCAGCCTCGGCATGGACATCCATGCATCAGACCTGAACCTCCTCACTCGGAGG AAACTGAGTCGCCTGCTGGACCCGCCCGACCCCCTGGGGAAGGACTGGTGCCTTCTCGCCATGAACTTA GGCCTCCCTGACCTCGTGGCAAAGTACAACACCAGTAACGGGGCTCCCAAGGATTTCCTCCCCAGCCCC CTCCACGCCCTGCTGCGGGAATGGACCACCTACCCTGAGAGCACAGTGGGCACCCTCATGTCCAAACTG AGGGAGCTGGGTCGCCGGGATGCCGCAGACTTTTTGCTGAAGGCATCCTCTGTGTTCAAAATCAACCTG GATGGCAATGGCCAGGAGGCCTATGCCTCGAGCTGCAACAGCGGCACCTCTTACAATTCCATTAGCTCT GTTGTATCCCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG240141
Blue=ORF Red=Cloning site Green=Tag(s) MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILK EIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHF DLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVIT YILLSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIK PKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVM KAIIHAINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDVQDKGGSN AVYWAARHGHVDTLKFLSENKCPLDVKDKSGEMALHVAARYGHADVAQLLCSFGSNPNIQDKEEETPLH CAAWHGYYSVAKALCEAGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNACDKDGHIALHLAV RRCQMEVIKTLLSQGCFVDYQDRHGNTPLHVACKDGNMPIVVALCEANCNLDISNKYGRTPLHLAANNG ILDVVRYLCLMGASVEALTTDGKTAEDLARSEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKL KLFGHSGSGKTTLVESLKCGLLRSFFRRRRPRLSSTNSSRFPPSPLASKPTVSVSINNLYPGCENVSVR SRSMMFEPGLTKGMLEVFVAPTHHPHCSADDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFA ANDPTSIHVVVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLQVVLVATHADIMNVPRP AGGEFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLC EKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEEDLRRIAQQLHSTGEINIMQSETVQDVLLL DPRWLCTNVLGKLLSVETPRALHHYRGRYTVEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDVP ALIKTDNLHRSWADEEDEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDADIRLWVNG CKLANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCLLLDSVCSTIENVMATTLPGLLTVKHYLSPQQL REHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRR KLSRLLDPPDPLGKDWCLLAMNLGLPDLVAKYNTSNGAPKDFLPSPLHALLREWTTYPESTVGTLMSKL RELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001288731 |
| ORF Size | 4290 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001288731.2 |
| RefSeq Size | 5760 bp |
| RefSeq ORF | 4293 bp |
| Locus ID | 1612 |
| UniProt ID | P53355 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Bladder cancer, Pathways in cancer |
| MW | 160.5 kDa |
| Gene Summary | Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
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