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Josephin 2 (JOSD2) (NM_001270640) Human Tagged ORF Clone
CAT＃: RG232033
- TrueORF®
JOSD2 (tGFP-tagged) - Homo sapiens Josephin domain containing 2 (JOSD2), transcript variant 2
ORF Plasmid: DDK
"NM_001270640" in other vectors (2)
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CNY 4,370.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SBBI54 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232033 representing NM_001270640
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGGCCCCGGGAGCACAGCCGAGCCCACCCACCGTGTACCACGAACGGCAGCGCCTGGAGCTGT GTGCTGTCCACGCCCTCAACAACGTTCTGCAGCAGCAGCTCTTTAGCCAGGAGGCTGCCGATGAGATCTG CAAGAGGTTGGCCCCAGACTCCCGGCTGAACCCTCATCGCAGCCTCCTGGGCACCGGCAACTATGATGTC AATGTGATCATGGCCGCTCTGCAGGGGCTGGGCCTGGCCGCCGTGTGGTGGGACAGGAGGAGGCCCCTGT CCCAGCTGGCCCTGCCCCAGGTACTGGGGCTGATCCTGAACCTGCCCTCGCCCGTGTCGCTGGGGCTGCT GTCACTGCCGCTGCGCCGGCGGCACTGGGTGGCCCTGCGCCAGGTGGACGGTGTCTACTACAACCTGGAC TCCAAGCTGCGGGCGCCCGAGGCCCTGGGGGATGAGGACGGAGTCAGGGCCTTCCTGGCGGCTGCGCTGG CCCAGGGCCTGTGCGAGGTGCTGCTGGTAGTGACCAAGGAGGTGGAGGAGAAGGGCAGCTGGCTGCGGAC AGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232033 representing NM_001270640
Red=Cloning site Green=Tags(s) MSQAPGAQPSPPTVYHERQRLELCAVHALNNVLQQQLFSQEAADEICKRLAPDSRLNPHRSLLGTGNYDV NVIMAALQGLGLAAVWWDRRRPLSQLALPQVLGLILNLPSPVSLGLLSLPLRRRHWVALRQVDGVYYNLD SKLRAPEALGDEDGVRAFLAAALAQGLCEVLLVVTKEVEEKGSWLRTD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001270640 |
| ORF Size | 564 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001270640.1, NP_001257569.1 |
| RefSeq Size | 1014 bp |
| RefSeq ORF | 567 bp |
| Locus ID | 126119 |
| UniProt ID | Q8TAC2 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein containing a Josephin domain. Josephin domain-containing proteins are deubiquitinating enzymes which catalyze the hydrolysis of the bond between the C-terminal glycine of the ubiquitin peptide and protein substrates. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
Documents
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Resources
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