SGT1 (ECD) (NM_001135752) Human Tagged ORF Clone

CAT＃: RG227241

TrueORF^®

ECD (tGFP-tagged) - Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 2

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_001135752" in other vectors (4)

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CNY 7,980.00

货期*

3周

规格

Product images

经常一起买 (5)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA366049

ECD rabbit polyclonal antibody

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	GCR2; HSGT1; SGT1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG227241 representing NM_001135752 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAACCATGAAGCTTGCTACGATGGAAGACACAGTGGAGTACTGCCTGTTCCTGATACCAGATG AGTCAAGGGACTCAGATAAACATAAAGAGATTCTTCAGAAGTACATTGAGAGAATAATCACTCGGTTTGC ACCTATGCTGGTCCCCTACATCTGGCAGAATCAGCCTTTCAATCTTAAATATAAACCTGGGAAAGGAGGT GTTCCTGCTCATATGTTTGGCGTGACAAAGTTTGGGGATAACATTGAGGATGAATGGTTTATTGTTTATG TAATAAAGCAGATCACAAAGGAATTTCCAGAGTTAGTAGCAAGGATTGAAGACAATGATGGTGAATTCTT GTTAATAGAAGCTGCTGACTTTCTCCCTAAATGGCTGGATCCTGAAAATAGCACCAATAGGGTATTTTTC TGCCATGGGGAATTGTGTATTATCCCTGCACCAAGAAAATCTGGAGCAGAATCTTGGTTACCCACCACAC CCCCAACAATTCCACAAGCATTGAATATAATCACAGCACATTCAGAAAAAATACTTGCTTCAGAATCTAT ACGAGCTGCTGTGAATAGGCGCATCAGAGGGTACCCAGAAAAAATTCAGGCCTCACTTCATCGAGCACAC TGCTTCCTTCCAGCTGGCATTGTGGCAGTGCTAAAGCAGCGCCCCAGATTGGTGGCTGCAGCAGTCCAGG CATTTTACCTACGAGACCCTATTGACCTGCGAGCTTGTCGTGTTTTCAAGACATTCTTGCCTGAAACACG AATAATGACATCGGTCACATTCACTAAATGTCTATATGCACAATTGGTGCAACAAAGGTTTGTGCCAGAC CGGCGGAGTGGATACAGGCTGCCTCCTCCATCTGATCCCCAGTACCGAGCCCATGAATTGGGCATGAAAT TGGCTCATGGATTTGAGATCTTATGCTCCAAATGTAGCCCACATTTTTCTGACTGCAAGAAATCCCTTGT GACTGCCTCACCACTCTGGGCCAGTTTCCTTGAAAGTCTGAAAAAGAATGATTACTTTAAGGGACTGATA GAAGGTTCTGCTCAGTACCGGGAAAGGCTAGAAATGGCAGAGAATTACTTCCAGCTCTCAGTAGACTGGC CAGAAAGGTTGGAGGTGCAGTGGCGCGATCCTGGCTTACTGCAAGCTCCACCTCCTGGGTTCACGCCATT CATCTGCCTCAGCCTCCTGAGTACCTGGGACAACAGTTCTCTTGCTATGAGCCCTGGTGAAGAAATCTTA ACCTTATTACAGACAATACCATTTGATATAGAAGACCTTAAGAAAGAAGCAGCTAATCTTCCCCCAGAGG ATGATGACCAGTGGTTAGATCTCTCACCAGATCAGCTGGACCAGCTGCTGCAGGAAGCTGTTGGCAAAAA AGAATCCGAGTCTGTTTCCAAGGAGGAGAAGGAGCAGAACTATGACTTAACTGAAGTCTCAGAGAGCATG AAAGCTTTCATATCCAAAGTCTCAACCCACAAGGGAGCAGAGCTGCCTCGAGAACCTTCTGAGGCTCCAA TCACTTTTGATGCAGATTCTTTTCTTAATTATTTTGATAAGATTTTAGGGCCAAGGCCTAATGAGTCAGA TTCTGATGATCTGGATGATGAAGACTTTGAATGTTTAGATAGTGATGATGACTTGGACTTTGAAACACAC GAACCTGGCGAAGAGGCTTCCCTGAAAGGAACACTTGATAATCTCAAGTCATACATGGCCCAGATGGACC AGGAACTAGCACACACCTGCATCAGCAAAAGTTTCACCACTAGGAACCAAGTGGAACCTGTATCCCAGAC TACCGATAACAATTCAGATGAGGAAGATTCTGGTACGGGAGAATCTGTTATGGCACCAGTAGATGTAGAC CTGAACCTGGTTTCAAATATATTGGAATCCTATAGCTCCCAAGCTGGACTGGCAGGACCTGCTTCCAATC TTTTACAAAGCATGGGAGTGCAGCTGCCTGACAACACCGATCACAGACCAACAAGTAAGCCAACAAAAAA T ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG227241 representing NM_001135752 Red=Cloning site Green=Tags(s) MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGG VPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIEAADFLPKWLDPENSTNRVFF CHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAH CFLPAGIVAVLKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSVTFTKCLYAQLVQQRFVPD RRSGYRLPPPSDPQYRAHELGMKLAHGFEILCSKCSPHFSDCKKSLVTASPLWASFLESLKKNDYFKGLI EGSAQYRERLEMAENYFQLSVDWPERLEVQWRDPGLLQAPPPGFTPFICLSLLSTWDNSSLAMSPGEEIL TLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESM KAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETH EPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVD LNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001135752
ORF Size	2031 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001135752.1, NP_001129224.1
RefSeq Size	2379 bp
RefSeq ORF	2034 bp
Locus ID	11319
UniProt ID	O95905
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC227241	ECD (Myc-DDK-tagged)-Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,960.00 CNY 7,220.00
RC227241L3	Lenti-ORF clone of ECD (Myc-DDK-tagged)-Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,120.00
RC227241L4	Lenti-ORF clone of ECD (mGFP-tagged)-Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,120.00
SC326167	ECD (untagged)-Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 13,110.00

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