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UBE1C (UBA3) (NM_198197) Human Tagged ORF Clone
CAT＃: RG224294
- TrueORF®
UBE1C (tGFP-tagged) - Human ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) (UBE1C), transcript variant 3
ORF Plasmid: DDK
"NM_198197" in other vectors (4)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | UBA3, hUba3, MGC22384, DKFZp566J164 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224294 representing NM_198197
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACTATAGATGTTTCCAATCTAAATAGGCAGTTTTTATTTAGGCCTAAAGATATTGGAAGACCTA AGGCTGAAGTTGCTGCAGAATTTCTAAATGACAGAGTTCCTAATTGCAATGTAGTTCCACATTTCAACAA GATTCAAGATTTTAACGACACTTTCTATCGACAATTTCATATTATTGTATGTGGACTGGACTCTATCATC GCCAGAAGATGGATAAATGGCATGCTGATATCTCTTCTAAATTATGAAGATGGTGTCTTAGATCCAAGCT CCATTGTCCCTTTGATAGATGGGGGGACAGAAGGTTTTAAAGGAAATGCCCGGGTGATTCTGCCTGGAAT GACTGCTTGTATCGAATGCACGCTGGAACTTTATCCACCACAGGTTAATTTTCCCATGTGCACCATTGCA TCTATGCCCAGGCTACCAGAACACTGTATTGAGTATGTAAGGATGTTGCAGTGGCCTAAGGAGCAGCCTT TTGGAGAAGGGGTTCCATTAGATGGAGATGATCCTGAACATATACAATGGATTTTCCAAAAATCCCTAGA GAGAGCATCACAATATAATATTAGGGGTGTTACGTATAGGCTCACTCAAGGGGTAGTAAAAAGAATCATT CCTGCAGTAGCTTCCACAAATGCAGTCATTGCAGCTGTGTGTGCCACTGAGGTTTTTAAAATAGCCACAA GTGCATACATTCCCTTGAATAATTACTTGGTGTTTAATGATGTAGATGGGCTGTATACATACACATTTGA AGCAGAAAGAAAGGAAAACTGCCCAGCTTGTAGCCAGCTTCCTCAAAATATTCAGTTTTCTCCATCAGCT AAACTACAGGAGGTTTTGGATTATCTAACCAATAGTGCTTCTCTGCAAATGAAATCTCCAGCCATCACAG CCACCCTAGAGGGAAAAAATAGAACACTTTACTTACAGTCGGTAACCTCTATTGAAGAACGAACAAGGCC AAATCTCTCCAAAACATTGAAAGAATTGGGGCTTGTTGATGGACAAGAACTGGCGGTTGCTGATGTCACC ACCCCACAGACTGTACTATTCAAACTTCATTTTACTTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224294 representing NM_198197
Red=Cloning site Green=Tags(s) MDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSII ARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIA SMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRII PAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSA KLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVT TPQTVLFKLHFTS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198197 |
| ORF Size | 1089 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198197.1, NP_937840.1 |
| RefSeq Size | 2015 bp |
| RefSeq ORF | 1091 bp |
| Locus ID | 9039 |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224294 | UBE1C (Myc-DDK-tagged)-Human ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) (UBE1C), transcript variant 3 |
CNY 3,656.00 |
|
| RC224294L3 | Lenti ORF clone of Human ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) (UBE1C), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224294L4 | Lenti ORF clone of Human ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) (UBE1C), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| SC127005 | UBE1C (untagged)-Human ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) (UBE1C), transcript variant 3 |
CNY 3,656.00 |
