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Calpain 3 (CAPN3) (NM_173090) Human Tagged ORF Clone
CAT＃: RG222089
- TrueORF®
CAPN3 (tGFP-tagged) - Human calpain 3, (p94) (CAPN3), transcript variant 6
ORF Plasmid: DDK
"NM_173090" in other vectors (4)
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CNY 2,800.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CANP3; CANPL3; LGMD2; LGMD2A; LGMDD4; LGMDR1; nCL-1; p94 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222089 representing NM_173090
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGA CACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCT CAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACACTATGAC ACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCA ACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTT CATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGT ATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222089 representing NM_173090
Red=Cloning site Green=Tags(s) MEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYD TDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDG IIKLNVLEWLQLTMYA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173090 |
| ORF Size | 468 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173090.1, NP_775113.1 |
| RefSeq Size | 1309 bp |
| RefSeq ORF | 471 bp |
| Locus ID | 825 |
| UniProt ID | P20807 |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222089 | CAPN3 (Myc-DDK-tagged)-Human calpain 3, (p94) (CAPN3), transcript variant 6 |
CNY 1,200.00 |
|
| RC222089L3 | Lenti ORF clone of Human calpain 3, (p94) (CAPN3), transcript variant 6, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222089L4 | Lenti ORF clone of Human calpain 3, (p94) (CAPN3), transcript variant 6, mGFP tagged |
CNY 5,890.00 |
|
| SC126949 | CAPN3 (untagged)-Human calpain 3, (p94) (CAPN3), transcript variant 6 |
CNY 1,200.00 |
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