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GCOM1 (POLR2M) (NM_001018102) Human Tagged ORF Clone
CAT＃: RG221814
- TrueORF®
POLR2M (tGFP-tagged) - Human glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A), transcript variant 2
ORF Plasmid: DDK
"NM_001018102" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GCOM1; Gdown; Gdown1; GRINL1A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221814 representing NM_001018102
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCTCGCTGCCCCGCGGCTTCGAGCCCCAAGCTCCCGAGGACTTGGCGCAGCGGAGTTTGGTGGAGC TGCGGGAAATGTTGAAGCGCCAGGAGAGACTTTTGCGCAACGAGTTACAGAGGATCACCATTGCGGACCA AGGTGAACAACAGTCAGAAGAAAACGCAAGTACTAAGAACTTGACAGGCCTTTCCAGTGGGACTGAGAAG AAACCTCATTACATGGAAGTGCTAGAAATGCGAGCCAAAAACCCAGTGCCCCAGCTGCGTAAATTTAAAA CCAATGTGTTACCTTTTCGACAAAATGATTCATCTAGTCATTGCCAGAAGAGTGGGTCTCCTATTTCCTC AGAAGAGCGGCGGCGCAGGGATAAGCAGCATCTTGATGACATCACAGCAGCTCGGCTTCTACCACTTCAC CATATGCCCACGCAGCTGCTCTCCATAGAAGAATCCTTGGCACTTCAGAAACAGCAGAAACAGAATTATG AGGAGATGCAAGCAAAGCTCGCAGCGCAAAAATTAGCTGAAAGACTGAATATTAAAATGCGGAGTTATAA TCCAGAAGGGGAGTCTTCAGGGAGATACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGAA TTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221814 representing NM_001018102
Red=Cloning site Green=Tags(s) MCSLPRGFEPQAPEDLAQRSLVELREMLKRQERLLRNELQRITIADQGEQQSEENASTKNLTGLSSGTEK KPHYMEVLEMRAKNPVPQLRKFKTNVLPFRQNDSSSHCQKSGSPISSEERRRRDKQHLDDITAARLLPLH HMPTQLLSIEESLALQKQQKQNYEEMQAKLAAQKLAERLNIKMRSYNPEGESSGRYREVRDEDDDWSSDE F TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001018102 |
| ORF Size | 633 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001018102.2 |
| RefSeq Size | 3668 bp |
| RefSeq ORF | 636 bp |
| Locus ID | 81488 |
| UniProt ID | P0CAP1 |
| Protein Families | Druggable Genome, Ion Channels: Other |
| Gene Summary | This gene encodes a subunit of a specific form of RNA polymerase II termed Pol II(G). The encoded protein may act as a negative regulator of transcriptional activation by the Mediator complex. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 4. Readthrough transcription between this gene and the neighboring upstream gene MYZAP (myocardial zonula adherens protein) is represented with GeneID 145781. [provided by RefSeq, Oct 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221814 | POLR2M (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A), transcript variant 2 |
CNY 3,990.00 |
|
| RC221814L3 | Lenti-ORF clone of POLR2M (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A), transcript variant 2 |
CNY 5,890.00 |
|
| RC221814L4 | Lenti-ORF clone of POLR2M (mGFP-tagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A), transcript variant 2 |
CNY 5,890.00 |
|
| SC302184 | POLR2M (untagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A), transcript variant 2 |
CNY 3,990.00 |
