SARA (ZFYVE9) (NM_007323) Human Tagged ORF Clone

CAT＃: RG219804

TrueORF^®

ZFYVE9 (tGFP-tagged) - Human zinc finger, FYVE domain containing 9 (ZFYVE9), transcript variant 2

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_007323" in other vectors (4)

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CNY 8,360.00

货期*

5周

规格

Product images

经常一起买 (5)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA383493

ZFYVE9 Rabbit polyclonal Antibody

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	MADHIP; NSP; SARA; SMADIP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG219804 representing NM_007323 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAATTACTTCCAAGCAGAAGCTTACAACCTGGACAAGGTGTTAGATGAATTTGAACAAAACGAAG ATGAAACAGTTTCTTCTACTTTATTGGATACAAAGTGGAATAAGATTCTAGATCCCCCTTCTCACCGGCT GTCATTTAACCCTACTTTGGCCAGTGTGAATGAATCTGCAGTTTCTAATGAGTCACAACCACAACTGAAA GTCTTCTCCCTGGCTCATTCAGCTCCCCTGACCACAGAGGAAGAGGATCACTGTGCTAATGGACAGGACT GTAATCTAAATCCAGAGATTGCCACAATGTGGATTGATGAAAATGCTGTTGCAGAAGACCAGTTAATTAA GAGAAACTATAGTTGGGATGATCAATGCAGTGCTGTTGAAGTGGGAGAGAAGAAATGTGGAAACCTGGCT TGTCTGCCAGATGAGAAGAATGTTCTTGTTGTAGCCGTCATGCATAACTGTGATAAAAGGACATTACAAA ACGATTTACAGGATTGTAATAATTATAATAGTCAATCCCTTATGGATGCTTTTAGCTGTTCACTGGATAA TGAAAACAGACAAACTGATCAATTTAGTTTTAGTATAAATGAGTCCACTGAAAAAGATATGAATTCAGAG AAACAAATGGATCCATTGAATAGACCGAAAACAGAGGGGAGATCTGTTAACCATCTGTGTCCTACTTCAT CTGATAGTCTAGCCAGTGTCTGTTCCCCTTCACAATTAAAGGATGACGGAAGTATAGGTAGAGACCCCTC CATGTCTGCGATTACAAGTTTAACGGTTGATTCAGTAATCTCATCCCAGGGAACAGATGGATGTCCTGCT GTTAAAAAGCAAGAGAACTATATACCAGATGAGGACCTCACTGGCAAAATCAGCTCTCCTAGGACAGATC TAGGGAGTCCAAATTCCTTTTCCCACATGAGTGAGGGGATTTTGATGAAAAAAGAGCCAGCAGAGGAGAG CACCACTGAAGAATCCCTCCGGTCTGGTTTACCTTTGCTTCTCAAACCAGACATGCCTAATGGGTCTGGA AGGAATAATGACTGTGAACGGTGTTCAGATTGCCTTGTGCCTAATGAAGTTAGGGCTGATGAAAATGAAG GTTATGAACATGAAGAAACTCTTGGCACTACAGAATTCCTTAATATGACAGAGCATTTCTCTGAATCTCA GGACATGACTAATTGGAAGTTGACTAAACTAAATGAGATGAATGATAGCCAAGTAAACGAAGAAAAGGAA AAGTTTCTACAGATTAGTCAGCCTGAGGACACTAATGGTGATAGTGGAGGACAGTGTGTTGGATTGGCAG ATGCAGGTCTAGATTTAAAAGGAACTTGCATTAGTGAAAGTGAAGAATGTGATTTCTCCACTGTTATAGA CACACCAGCAGCAAATTATCTATCTAATGGTTGTGATTCCTATGGAATGCAAGACCCAGGTGTTTCTTTT GTTCCAAAGACTTTACCCTCCAAAGAAGATTCAGTAACAGAAGAAAAAGAAATAGAGGAAAGCAAGTCAG AATGCTACTCAAATATTTATGAACAGAGAGGAAATGAGGCCACAGAAGGGAGTGGACTACTTTTAAACAG CACTGGTGACCTAATGAAGAAAAATTATTTACATAATTTCTGTAGTCAAGTTCCATCAGTGCTTGGGCAA TCTTCCCCCAAGGTAGTAGCAAGCCTGCCATCTATCAGTGTTCCTTTTGGTGGTGCAAGACCCAAGCAAC CTTCTAATCTTAAACTTCAAATTCCAAAGCCATTATCAGACCATTTACAAAATGACTTTCCTGCAAACAG TGGAAATAATACTAAAAATAAAAATGATATTCTTGGGAAAGCAAAATTAGGGGAAAACTCAGCAACCAAT GTATGCAGTCCATCTTTGGGAAACATCTCTAATGTCGATACAAATGGGGAACATTTAGAAAGTTATGAGG CTGAGATCTCCACTAGACCATGCCTTGCATTAGCTCCAGATAGCCCAGATAATGATCTCAGAGCTGGTCA GTTTGGAATTTCTGCCAGAAAGCCATTCACCACTCTGGGTGAGGTGGCTCCAGTATGGGTACCGGATTCT CAGGCTCCAAATTGCATGAAATGTGAAGCCAGGTTTACATTCACCAAAAGGAGGCATCACTGCAGAGCAT GTGGGAAGGTTTTCTGTGCTTCCTGCTGTAGCCTGAAATGTAAACTGTTATACATGGACAGAAAGGAAGC TAGAGTGTGTGTAATCTGCCATTCAGTGCTAATGAATGGTAAGTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG219804 representing NM_007323 Red=Cloning site Green=Tags(s) MENYFQAEAYNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPPSHRLSFNPTLASVNESAVSNESQPQLK VFSLAHSAPLTTEEEDHCANGQDCNLNPEIATMWIDENAVAEDQLIKRNYSWDDQCSAVEVGEKKCGNLA CLPDEKNVLVVAVMHNCDKRTLQNDLQDCNNYNSQSLMDAFSCSLDNENRQTDQFSFSINESTEKDMNSE KQMDPLNRPKTEGRSVNHLCPTSSDSLASVCSPSQLKDDGSIGRDPSMSAITSLTVDSVISSQGTDGCPA VKKQENYIPDEDLTGKISSPRTDLGSPNSFSHMSEGILMKKEPAEESTTEESLRSGLPLLLKPDMPNGSG RNNDCERCSDCLVPNEVRADENEGYEHEETLGTTEFLNMTEHFSESQDMTNWKLTKLNEMNDSQVNEEKE KFLQISQPEDTNGDSGGQCVGLADAGLDLKGTCISESEECDFSTVIDTPAANYLSNGCDSYGMQDPGVSF VPKTLPSKEDSVTEEKEIEESKSECYSNIYEQRGNEATEGSGLLLNSTGDLMKKNYLHNFCSQVPSVLGQ SSPKVVASLPSISVPFGGARPKQPSNLKLQIPKPLSDHLQNDFPANSGNNTKNKNDILGKAKLGENSATN VCSPSLGNISNVDTNGEHLESYEAEISTRPCLALAPDSPDNDLRAGQFGISARKPFTTLGEVAPVWVPDS QAPNCMKCEARFTFTKRRHHCRACGKVFCASCCSLKCKLLYMDRKEARVCVICHSVLMNGKY TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_007323
ORF Size	2286 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_007323.1, NP_015562.1
RefSeq Size	2625 bp
RefSeq ORF	2288 bp
Locus ID	9372
Domains	FYVE
Protein Pathways	TGF-beta signaling pathway
Gene Summary	This gene encodes a double zinc finger motif-containing protein that participates in the transforming growth factor-beta (TGFB) signalling pathway. The encoded protein interacts directly with SMAD2 and SMAD3, and recruits SMAD2 to the TGFB receptor. There are multiple pseudogenes for this gene on chromosomes 2, 15, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC219804	ZFYVE9 (Myc-DDK-tagged)-Human zinc finger, FYVE domain containing 9 (ZFYVE9), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,600.00
RC219804L3	Lenti-ORF clone of ZFYVE9 (Myc-DDK-tagged)-Human zinc finger, FYVE domain containing 9 (ZFYVE9), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,500.00
RC219804L4	Lenti-ORF clone of ZFYVE9 (mGFP-tagged)-Human zinc finger, FYVE domain containing 9 (ZFYVE9), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,500.00
SC314638	ZFYVE9 (untagged)-Human zinc finger, FYVE domain containing 9 (ZFYVE9), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,730.00

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