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Myosin Heavy chain 1 (MYH1) (NM_005963) Human Tagged ORF Clone
CAT＃: RG218544
- TrueORF®
MYH1 (tGFP-tagged) - Human myosin, heavy chain 1, skeletal muscle, adult (MYH1)
ORF Plasmid: DDK
"NM_005963" in other vectors (2)
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CNY 20,900.00
货期*
8周
规格
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经常一起买 (5)
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218544 representing NM_005963
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCTGACTCTGAGATGGCCATTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCTGAAAGGGAGC GAATTGAAGCCCAGAACAAGCCTTTTGATGCCAAGACATCAGTCTTTGTGGTGGACCCTAAGGAGTCCTT TGTGAAAGCAACAGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCTAAGACCGAAGCTGGAGCTACTGTA ACAGTGAAAGATGACCAAGTCTTCCCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGGCCATGA TGACTCATCTACACGAGCCTGCTGTGCTGTACAACCTCAAAGAGCGCTACGCAGCCTGGATGATCTACAC CTACTCAGGCTTGTTCTGTGTCACTGTCAACCCCTACAAGTGGTTGCCAGTGTATAATGCAGAAGTGGTG ACAGCCTACCGAGGCAAAAAGCGCCAGGAAGCCCCACCCCACATCTTCTCCATCTCTGACAATGCCTATC AGTTCATGCTGACTGATCGGGAGAATCAGTCTATCTTGATCACCGGAGAATCTGGCGCAGGGAAGACTGT GAACACCAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTTACTGGGGAGAAGAAGAAGGAAGAAGTT ACTTCTGGCAAAATGCAGGGGACTCTGGAAGATCAAATCATCAGTGCCAACCCCCTACTGGAGGCCTTTG GCAACGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATCCACTTCGGTAC CACAGGGAAACTGGCTTCTGCTGATATTGAAACATATCTTCTGGAGAAGTCTAGAGTTACTTTCCAGCTA AAGGCTGAAAGAAGCTATCATATTTTTTATCAGATCATGTCTAACAAGAAGCCAGATCTAATTGAAATGC TCCTGATCACCACCAACCCATACGATTATGCCTTCGTCAGTCAAGGGGAGATCACAGTGCCCAGCATTGA TGACCAAGAAGAGTTGATGGCTACAGATAGTGCCATTGAAATTCTGGGCTTTACTTCAGATGAAAGAGTG TCCATCTATAAGCTCACAGGGGCTGTGATGCATTATGGGAACATGAAATTCAAGCAAAAGCAGCGTGAGG AGCAAGCTGAGCCAGATGGCACTGAAGTTGCTGACAAGGCAGCCTATCTCCAAAATCTGAACTCTGCAGA TCTGCTCAAAGCCCTCTGCTACCCTAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGTCAAACTGTG CAGCAGGTGTACAATGCAGTGGGTGCTCTGGCCAAAGCTGTCTACGATAAGATGTTCTTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATTGGGGTCTTGGACATTGCTGG CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATTGAGTGGACGTTCA TTGACTTTGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAACAACATCTT GGAAAATCCAATAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAGCCTGAGGCCCACTTCTCTTTGATTC ACTATGCTGGCACCGTGGACTACAACATTGCCGGCTGGCTTGACAAGAACAAGGACCCCCTGAATGAGAC TGTGGTGGGGCTGTACCAGAAGTCTGCAATGAAGACTCTGGCTCTCCTCTTTGTTGGGGCAACGGGAGCG GAAGCAGAGGCTGGCGGTGGAAAGAAAGGTGGTAAGAAGAAGGGTTCTTCTTTCCAGACTGTGTCGGCTC TCTTCAGGGAGAATTTGAATAAGCTGATGACCAACTTGAGGAGCACTCACCCCCACTTTGTGCGGTGCAT CATCCCCAATGAAACTAAAACTCCTGGTGCCATGGAGCATGAGCTTGTCCTGCATCAGCTGAGGTGTAAC GGTGTGCTGGAAGGCATCCGCATCTGCAGGAAAGGCTTCCCAAGCAGAATCCTTTATGCAGACTTCAAAC AGAGATACAAGGTGTTAAATGCAAGTGCTATCCCTGAAGGACAATTCATCGATAGCAAGAAGGCTTCAGA GAAGCTCCTGGGGTCCATTGACATTGACCACACCCAGTATAAATTTGGTCACACCAAGGTCTTTTTCAAA GCTGGTCTTCTGGGGCTCCTAGAGGAGATGCGAGATGAGAAGCTGGCCCAGCTGATTACCCGAACCCAGG CCATGTGCAGAGGGTTCTTGGCAAGAGTGGAGTACCAGAAAATGGTGGAAAGAAGAGAGTCCATCTTCTG CATCCAGTACAATGTCCGTGCCTTCATGAATGTCAAGCACTGGCCCTGGATGAAGCTGTATTTCAAGATC AAACCCCTCCTCAAAAGTGCAGAGACAGAGAAGGAGATGGCCAACATGAAGGAAGAATTTGAGAAAACCA AAGAAGAGCTGGCTAAGACCGAGGCAAAAAGGAAAGAGCTGGAAGAAAAAATGGTGACTCTGATGCAAGA AAAAAATGACTTGCAACTCCAGGTTCAAGCTGAAGCTGACAGCTTGGCTGATGCAGAGGAAAGGTGTGAC CAGCTAATCAAAACCAAAATCCAGCTAGAAGCCAAAATCAAAGAGGTGACTGAGAGAGCTGAGGATGAGG AAGAGATCAATGCTGAGCTGACAGCCAAGAAGAGGAAACTGGAGGATGAATGTTCAGAACTCAAGAAAGA CATTGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACAGAAAACAAGGTGAAA AACCTCACAGAAGAGATGGCGGGTCTGGATGAAACCATTGCTAAGCTGACCAAGGAGAAGAAGGCTCTCC AGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGC TAAAATCAAACTTGAACAACAAGTGGATGATCTTGAAGGATCTTTGGAACAAGAAAAGAAAATCCGGATG GATCTAGAAAGAGCAAAGAGAAAACTAGAGGGAGACCTAAAATTGGCTCAAGAATCCGCAATGGATATAG AAAATGACAAACAACAACTTGATGAAAAGCTTAAAAAGAAAGAGTTTGAAATGAGCGGTCTGCAAAGCAA GATTGAAGATGAACAAGCCCTTGGTATGCAGCTGCAGAAGAAAATCAAGGAGTTACAAGCCCGCATTGAG GAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAGCAGCGCTCTGATCTCT CCCGGGAGCTGGAGGAGATCAGTGAGAGGCTGGAAGAAGCCGGTGGGGCCACCTCGGCCCAGATTGAGAT GAACAAGAAGCGGGAAGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACCCTACAGCATGAG GCCACGGCGGCCACCCTGAGGAAGAAGCATGCAGATAGTGTGGCCGAGCTTGGGGAGCAGATTGACAACC TGCAGCGAGTGAAGCAGAAGCTGGAGAAGGAGAAGAGTGAGATGAAGATGGAGATCGATGACCTTGCTAG TAACATGGAGACTGTCTCCAAAGCCAAGGGAAACCTTGAAAAGATGTGCCGCGCTCTAGAAGATCAACTG AGTGAAATTAAGACCAAGGAAGAGGAGCAGCAGCGGCTGATCAATGACCTCACAGCACAGAGAGCGCGCC TGCAAACAGAATCAGGTGAATATTCACGCCAGCTAGATGAAAAGGACACACTAGTTTCACAGCTCTCGAG GGGCAAACAAGCCTTTACTCAACAGATTGAGGAACTGAAAAGGCAACTTGAAGAGGAGATAAAGGCCAAG AGTGCCCTGGCACATGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAGGAGG AGCAGGAAGCCAAGGCCGAGCTACAGAGAGCAATGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGGAC CAAATATGAGACAGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCTCAGCGT CTGCAGGATGCTGAGGAACATGTAGAAGCTGTGAATGCCAAATGTGCTTCCCTTGAGAAGACGAAGCAGA GGCTCCAGAATGAAGTTGAGGACCTCATGATTGATGTTGAGAGGACAAATGCTGCCTGTGCCGCCCTGGA CAAAAAGCAAAGGAACTTTGATAAGATCCTGGCAGAATGGAAACAGAAGTGTGAAGAAACTCATGCTGAA CTTGAAGCTTCTCAAAAGGAATCCCGCTCACTCAGCACAGAACTATTTAAGATTAAGAATGCTTATGAGG AATCTTTAGACCAACTTGAAACCTTGAAACGGGAAAATAAGAATCTGCAGCAGGAGATTTCTGATCTCAC TGAACAGATTGCAGAAGGAGGAAAGCGCATCCATGAACTGGAAAAAATAAAGAAGCAAGTTGAGCAAGAA AAGTCTGAACTTCAGGCTGCCTTAGAGGAGGCAGAGGCATCTCTTGAACATGAAGAGGGAAAGATCCTGC GCATCCAGCTTGAGTTGAACCAAGTCAAGTCTGAGGTTGATAGGAAAATTGCTGAAAAAGATGAGGAAAT TGACCAGATGAAGAGAAACCACATTAGAATCGTGGAGTCCATGCAGAGCACACTGGATGCTGAGATCAGG AGCAGGAATGATGCCATTAGGCTCAAGAAGAAGATGGAGGGAGACCTCAATGAAATGGAAATCCAGCTGA ACCATGCCAACCGCATGGCTGCTGAGGCCCTGAGGAACTATAGGAACACCCAAGCCATCCTCAAGGATAC CCAGCTCCACCTAGATGATGCTCTCCGGAGCCAAGAGGACCTGAAGGAACAGCTGGCTATGGTGGAGCGC AGAGCCAACCTGCTGCAGGCTGAGATCGAGGAACTACGAGCCACTCTGGAACAGACGGAGAGGAGCAGGA AAATCGCAGAACAGGAGCTCCTGGATGCCAGTGAACGTGTTCAGCTCCTGCACACCCAGAACACCAGCCT GATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAAATCCAGGGAGAGATGGAAGACATCATCCAG GAAGCCCGCAATGCAGAAGAGAAGGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAGGAGCTGA AGAAGGAACAGGACACCAGCGCCCATCTGGAGCGGATGAAGAAGAACTTGGAACAGACGGTGAAGGACCT GCAGCATCGTCTGGATGAGGCTGAGCAGCTGGCCCTGAAGGGTGGGAAGAAGCAGATCCAGAAACTGGAG GCCAGGGTTCGTGAACTTGAAGGTGAAGTTGAAAGTGAACAGAAGCGCAATGTTGAAGCTGTCAAGGGTC TACGCAAACATGAGAGAAAAGTGAAGGAACTCACTTACCAAACTGAGGAAGACCGCAAGAATATTCTCAG GCTGCAGGACCTGGTGGACAAGCTGCAAGCAAAGGTGAAATCCTACAAGAGACAAGCTGAAGAAGCGGAG GAACAATCCAACGTCAACCTCTCCAAATTCCGGAGGATCCAGCACGAGCTGGAGGAGGCCGAGGAAAGGG CTGACATTGCTGAGTCCCAGGTCAACAAGCTGAGGGTGAAGAGCAGGGAGGTTCACACAAAAATCATAAG TGAAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218544 representing NM_005963
Red=Cloning site Green=Tags(s) MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATV TVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVV TAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEV TSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQL KAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERV SIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTV QQVYNAVGALAKAVYDKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ FFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHL GKSNNFQKPKPAKGKPEAHFSLIHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLALLFVGATGA EAEAGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCN GVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFK AGLLGLLEEMRDEKLAQLITRTQAMCRGFLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKI KPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADSLADAEERCD QLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVK NLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKIRM DLERAKRKLEGDLKLAQESAMDIENDKQQLDEKLKKKEFEMSGLQSKIEDEQALGMQLQKKIKELQARIE ELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHE ATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQL SEIKTKEEEQQRLINDLTAQRARLQTESGEYSRQLDEKDTLVSQLSRGKQAFTQQIEELKRQLEEEIKAK SALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQR LQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKCEETHAE LEASQKESRSLSTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQE KSELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQMKRNHIRIVESMQSTLDAEIR SRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQAILKDTQLHLDDALRSQEDLKEQLAMVER RANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIIQ EARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLE ARVRELEGEVESEQKRNVEAVKGLRKHERKVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAE EQSNVNLSKFRRIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005963 |
| ORF Size | 5817 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005963.2, NP_005954.2 |
| RefSeq Size | 5925 bp |
| RefSeq ORF | 5820 bp |
| Locus ID | 4619 |
| UniProt ID | P12882 |
| Protein Pathways | Tight junction, Viral myocarditis |
| Gene Summary | Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008] |
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