United States
Germany
Japan
United Kingdom
China
MYH7B (NM_020884) Human Tagged ORF Clone
CAT＃: RG217991
- TrueORF®
MYH7B (tGFP-tagged) - Human myosin, heavy chain 7B, cardiac muscle, beta (MYH7B)
ORF Plasmid: DDK
"NM_020884" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 15,200.00
货期*
3周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MHC14; MYH14 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217991 representing NM_020884.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTGGCAATAAAAGGGGTAGCAGAGCTTCCTGCCCTCACCGTGGTGCCGAGTGCCTGCTGCCTTGG GCCGCCTTGAACCTCCAGGGTTTCCAGCTCCTCCTCCTTCACCCCAGTGCCACTGCCATGATGGATGTG AGTGAACTTGGGGAGTCTGCCCGCTACCTCCGCCAGGGCTACCAGGAGATGACGAAGGTGCACACTATC CCATGGGACGGGAAGAAGCGAGTCTGGGTGCCTGATGAACAGGACGCCTACGTGGAGGCCGAGGTCAAG TCGGAGGCTACCGGGGGCAGAGTCACCGTGGAGACCAAAGACCAGAAGGTGCTGATGGTGCGTGAAGCC GAGCTGCAGCCCATGAACCCGCCTCGCTTCGACTTACTGGAGGACATGGCCATGATGACGCACCTGAAC GAGGCCTCTGTGCTGCACAACCTGCGCCAGCGCTATGCCCGCTGGATGATCTATACCTACTCAGGCCTC TTCTGTGTCACCATCAACCCCTACAAATGGCTCCCAGTCTATACGGCCTCCGTAGTGGCTGCTTACAAG GGAAAGCGCCGCTCAGATTCCCCGCCCCATATATATGCGGTGGCGGACAACGCCTACAACGACATGCTG CGCAACCGAGACAACCAGTCCATGCTGATCACCGGAGAGTCGGGGGCCGGTAAGACGGTTAACACCAAG CGGGTCATTCAGTACTTTGCCATCGTCGCTGCCCTGGGAGACGGGCCGGGCAAGAAGGCCCAATTTCTG GCAACAAAGACGGGGGGCACCCTTGAGGATCAAATCATCGAGGCCAACCCTGCCATGGAGGCCTTTGGC AACGCCAAGACCCTGAGGAATGATAACTCCTCCCGCTTTGGCAAGTTCATCCGCATTCACTTTGGTCCC TCTGGGAAGCTGGCATCCGCGGATATTGACAGCTATCTCCTGGAGAAGTCGCGGGTGATCTTCCAGTTG CCTGGTGAGCGCAGCTACCATGTCTACTACCAGATCCTCTCAGGGAGGAAGCCAGAGCTGCAGGACATG CTGCTTCTGTCTATGAACCCCTATGACTACCACTTCTGCAGCCAGGGCGTCATCACCGTGGACAACATG AATGATGGGGAGGAGCTCATCGCCACCGACCATGCCATGGACATCCTAGGCTTCAGCGTGGATGAGAAA TGTGCCTGCTATAAGATCGTGGGCGCCCTCCTGCACTTTGGCAACATGAAGTTCAAGCAGAAGCAGCGG GAGGAGCAGGCGGAGGCCGATGGCACTGAGAGTGCTGACAAGGCTGCCTACCTGATGGGGGTCAGCAGT GGGGACCTCCTCAAAGGCCTTTTGCACCCCCGGGTGCGTGTAGGGAACGAGTACGTGACCAAGGGCCAG AGTGTGGAGCAGGTGGTGTTTGCTGTGGGGGCTCTGGCCAAGGCCACCTATGACCGGCTGTTCAGGTGG CTGGTGTCTCGGATCAACCAGACCCTGGACACAAAGCTGCCCCGGCAGTTCTTCATCGGGGTTCTGGAC ATCGCTGGGTTTGAGATCTTTGAGTTCAACAGCTTCGAACAGCTGTGCATCAACTTCACCAATGAGAAA TTGCAGCAGTTCTTCAACCAGCACATGTTTGTGCTGGAGCAGGAGGAGTACAAGCGGGAGGGCATCGAC TGGGTCTTCATCGACTTCGGCCTTGACCTGCAGCCTTGCATCGACCTCATCGAGAAGCCACTGGGCATC CTGTCCATCCTGGAGGAGGAATGCATGTTCCCCAAGGCCTCAGACGCCAGCTTCCGGGCCAAGCTCTAC GACAACCACGCGGGGAAGTCACCCAATTTCCAGCAGCCTCGGCCTGACAAGAAGCGCAAGTACCAGGCC CACTTCGAGGTGGTCCACTACGCAGGCGTGGTGCCTTACAGCATTGTGGGCTGGCTGGAGAAAAACAAG GATCCCCTGAATGAGACCGTGGTCCCCATCTTCCAGAAGTCACAGAATAGGCTCCTGGCGACTCTCTAT GAGAATTATGCGGGCTCCTGCTCCACTGAGCCCCCCAAGTCTGGGGTGAAAGAGAAGCGTAAGAAGGCA GCATCGTTCCAGACGGTGTCCCAGCTGCACAAGGAGAACCTCAACAAGCTGATGACCAACCTGCGGGCC ACACAGCCCCACTTCGTCCGCTGCATTGTCCCCAACGAGAACAAAACCCCAGGGGTCATGGATGCCTTC TTGGTGCTACACCAGCTGCGCTGCAATGGGGTCCTGGAGGGGATCCGGATCTGCCGCCAAGGGTTCCCC AACAGGTTGCTCTACACCGACTTCCGGCAGCGGTACCGTATCCTGAACCCCAGTGCCATCCCGGATGAC ACCTTCATGGACAGCAGGAAGGCCACAGAGAAACTGCTGGGCTCGCTGGACTTGGATCACACCCAGTAC CAGTTTGGCCACACCAAGGTGTTCTTCAAGGCTGGGCTTCTAGGCGTCCTGGAAGAGCTCCGTGACCAG CGCCTGGCCAAGGTGCTGACGCTGCTGCAGGCGCGGAGCCGTGGCCGCCTCATGCGCCTTGAGTACCAG CGCCTGCTGGGAGGCAGGGATGCGCTGTTCACCATCCAGTGGAACATCCGTGCCTTCAATGCCGTCAAG AACTGGTCATGGATGAAGCTCTTTTTCAAGATGAAGCCGCTGCTGCGCTCGGCGCAGGCTGAGGAGGAG CTGGCGGCCCTGCGGGCAGAGCTGCGGGGGTTGCGAGGGGCGCTGGCTGCGGCCGAGGCCAAGCGCCAG GAACTGGAGGAGACGCACGTCAGCATCACCCAGGAGAAGAATGACCTGGCCCTGCAGCTGCAGGCTGAG CAGGACAACCTGGCAGATGCCGAGGAGCGCTGCCACTTGCTGATCAAGTCCAAGGTGCAGCTGGAGGGG AAGGTGAAGGAGCTGAGTGAGCGGCTGGAGGATGAGGAGGAGGTGAACGCTGACCTGGCCGCCCGCCGG CGCAAGCTGGAGGACGAGTGCACGGAGCTCAAGAAGGACATTGATGACCTGAAGCTGACACTGGCCAAA GCTGAGAAGGAGAAGCAAGCCACTGAGAACAAGGTGAAGAACCTGACGGAAGAGATGGCTGCGCTGGAC GAGTCAGTGGCCCGGCTGACCAAGGAGAAGAAGGCGTTGCAGGAGGCCCACCAACAGGCCCTGGGTGAC CTGCAGGCCGAGGAGGACCGTGTGAGCGCGCTGACCAAGGCCAAGCTCCGGCTGGAGCAACAGGTGGAG GACCTGGAATGCTCCCTGGAGCAGGAGAAGAAGCTGCGCATGGACACGGAGCGGGCCAAGCGCAAGCTG GAGGGTGACCTGAAGCTGACGCAGGAGTCGGTGGCTGATGCTGCTCAAGACAAGCAGCAGCTGGAGGAG AAGCTCAAGAAGAAGGACTCCGAGCTGAGCCAGCTGAGCCTGCGGGTGGAAGACGAGCAGCTCTTGGGG GCCCAGATGCAGAAGAAGATCAAGGAGCTGCAGGCTCGGGCGGAGGAGCTGGAAGAGGAGCTGGAGGCA GAGCGGGCAGCCCGGGCCCGCGTGGAGAAGCAGCGTGCAGAGGCGGCGCGGGAGCTGGAGGAGCTGAGC GAGCGGCTGGAGGAGGCAGGCGGCGCATCCGCGGGGCAGCGCGAGGGCTGCCGCAAGCGGGAGGCGGAG CTGGGGAGGCTGCGGCGGGAGCTGGAGGAGGCGGCGCTGCGGCACGAGGCCACAGTGGCGGCACTGCGG CGCAAGCAGGCGGAGGGCGCGGCGGAGCTGGGGGAGCAGGTGGACAGCCTGCAGCGGGTGCGGCAGAAG CTGGAGAAGGAGAAGAGTGAGCTGCGCATGGAGGTGGACGACCTGGCTGCCAACGTGGAGACTCTGACC CGCGCCAAGGCCAGTGCAGAGAAGCTGTGCCGGACCTATGAGGATCAGCTAAGCGAGGCCAAGATCAAG GTGGAGGAGCTGCAGCGGCAGCTGGCGGACGCAAGCACGCAGCGTGGGCGACTACAGACGGAAAGCGGG GAGCTGAGTCGCCTGCTAGAGGAGAAGGAGTGTCTGATCAGTCAGCTGAGCCGTGGAAAGGCCCTGGCC GCCCAAAGCCTGGAAGAGTTGCGGCGCCAGCTAGAGGAGGAAAGCAAGGCCAAGAGTGCCCTGGCCCAC GCCGTGCAGGCTCTGCGGCACGACTGTGACCTCCTGCGGGAGCAACACGAGGAGGAGGCTGAGGCCCAG GCTGAGCTGCAGCGGCTGCTGTCCAAGGCCAATGCCGAGGTGGCCCAGTGGAGGAGCAAGTACGAAGCA GATGCCATCCAGAGGACCGAGGAGCTGGAGGAGGCCAAAAAAAAGCTGGCACTGCGGCTGCAGGAGGCA GAGGAGGGCGTGGAGGCTGCCAACGCCAAGTGCTCATCGTTGGAGAAGGCCAAGCTGCGGCTACAGACA GAGTCAGAGGATGTAACCCTGGAGCTGGAGCGGGCGACCTCAGCAGCTGCTGCGCTGGACAAGAAGCAG CGGCACTTGGAACGGGCACTGGAGGAACGGCGGCGGCAGGAGGAGGAGATGCAGCGGGAGCTGGAGGCG GCACAGAGGGAGTCCCGTGGCCTGGGCACCGAGCTCTTCCGGCTGCGGCACGGCCACGAGGAGGCACTT GAAGCCCTGGAGACGCTCAAGCGGGAGAACAAGAACCTGCAGGAGGAGATCAGCGACCTCACAGACCAG GTGAGTCTCAGTGGGAAGAGCATCCAGGAACTGGAGAAAACCAAGAAGGCGCTGGAAGGCGAGAAGAGT GAGATCCAGGCTGCACTGGAGGAGGCAGAGGGGGCCCTGGAGCTGGAGGAGACCAAGACGCTGCGGATC CAGCTGGAGCTCTCCCAGGTCAAAGCAGAAGTGGACCGGAAGCTGGCAGAGAAAGACGAGGAGTGCGCT AACCTGAGGCGCAACCACCAGCGAGCTGTGGAGTCCCTGCAGGCCTCCCTGGATGCAGAGACACGGGCC CGCAATGAGGCGCTGCGGCTCAAGAAGAAGATGGAGGGTGACCTCAACGACCTGGAGCTGCAGCTGGGC CATGCCACCCGTCAGGCCACAGAGGCCCAGGCTGCCACGCGGCTGATGCAGGCACAGCTCAAGGAGGAG CAGGCAGGGCGGGACGAGGAGCAGCGGCTGGCAGCTGAGCTCCACGAGCAGGCGCAGGCTCTGGAGCGC CGGGCCTCGCTGCTGGCTGCGGAGCTGGAGGAGCTGCGGGCTGCCCTGGAGCAGGGCGAGCGCAGCCGG CGACTGGCAGAGCAGGAGCTTTTGGAGGCCACCGAGCGCCTCAACCTTCTGCATTCGCAGAACACAGGC CTCCTAAACCAGAAGAAGAAGCTGGAGGCGGACTTGGCCCAGCTGAGCGGGGAGGTGGAGGAGGCTGCA CAGGAGAGGCGGGAGGCTGAGGAGAAGGCCAAAAAGGCCATCACTGATGCGGCCATGATGGCCGAGGAG CTGAAGAAGGAGCAGGACACAAGTGCACACCTGGAACGGATGAAGAAGACGCTGGAGCAGACGGTGCGC GAGCTCCAGGCCCGCCTTGAGGAGGCAGAACAGGCCGCCCTCCGTGGCGGGAAGAAGCAGGTGCAGAAG CTGGAGGCCAAGGTACGGGAGCTGGAGGCTGAGCTTGATGCAGAGCAGAAGAAGCACGCCGAGGCCCTT AAGGGCGTGCGCAAGCATGAGCGCCGTGTCAAGGAGCTCGCATACCAGGCCGAGGAGGACAGGAAGAAC CTGGCTCGCATGCAGGACCTGGTGGACAAGCTGCAGAGCAAGGTCAAGAGCTACAAGCGCCAGTTTGAG GAGGCGGAGCAGCAGGCCAACACCAACCTGGCCAAGTATCGCAAGGCCCAGCACGAGCTGGATGATGCG GAGGAGCGGGCAGACATGGCGGAAACCCAGGCCAACAAGCTGCGGGCACGGACCCGGGACGCCCTGGGC CCCAAGCACAAGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG217991
Blue=ORF Red=Cloning site Green=Tag(s) MSGNKRGSRASCPHRGAECLLPWAALNLQGFQLLLLHPSATAMMDVSELGESARYLRQGYQEMTKVHTI PWDGKKRVWVPDEQDAYVEAEVKSEATGGRVTVETKDQKVLMVREAELQPMNPPRFDLLEDMAMMTHLN EASVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTASVVAAYKGKRRSDSPPHIYAVADNAYNDML RNRDNQSMLITGESGAGKTVNTKRVIQYFAIVAALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFG NAKTLRNDNSSRFGKFIRIHFGPSGKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDM LLLSMNPYDYHFCSQGVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQR EEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRW LVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGID WVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDNHAGKSPNFQQPRPDKKRKYQA HFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKA ASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFP NRLLYTDFRQRYRILNPSAIPDDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQ RLAKVLTLLQARSRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEE LAALRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEG KVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLKLTLAKAEKEKQATENKVKNLTEEMAALD ESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQVEDLECSLEQEKKLRMDTERAKRKL EGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQMQKKIKELQARAEELEEELEA ERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALR RKQAEGAAELGEQVDSLQRVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIK VEELQRQLADASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAH AVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEA EEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEA AQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKTKKALEGEKS EIQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECANLRRNHQRAVESLQASLDAETRA RNEALRLKKKMEGDLNDLELQLGHATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALER RASLLAAELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAA QERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQK LEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKLQSKVKSYKRQFE EAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPKHKE TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020884 |
| ORF Size | 5949 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020884.3 |
| RefSeq Size | 6289 bp |
| RefSeq ORF | 5826 bp |
| Locus ID | 57644 |
| UniProt ID | A7E2Y1 |
| Protein Families | Druggable Genome |
| Protein Pathways | Tight junction, Viral myocarditis |
| MW | 225.8 kDa |
| Gene Summary | The myosin II molecule is a multi-subunit complex consisting of two heavy chains and four light chains. This gene encodes a heavy chain of myosin II, which is a member of the motor-domain superfamily. The heavy chain includes a globular motor domain, which catalyzes ATP hydrolysis and interacts with actin, and a tail domain in which heptad repeat sequences promote dimerization by interacting to form a rod-like alpha-helical coiled coil. This heavy chain subunit is a slow-twitch myosin. Alternatively spliced transcript variants have been found, but the full-length nature of these variants is not determined. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
You may also need
Customer
Reviews
Loading...
