OR52K1 (NM_001005171) Human Tagged ORF Clone

CAT#: RG215464

  • TrueORF®

OR52K1 (tGFP-tagged) - Human olfactory receptor, family 52, subfamily K, member 1 (OR52K1)

ORF Plasmid: DDK tGFP



  "NM_001005171" in other vectors (4)

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CNY 5,200.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms OR11-8
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG215464 representing NM_001005171
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTCCCTCTAATATCACCTCAACACATCCAGCTGTCTTTTTGTTGGTAGGAATTCCTGGTTTGGAAC
ACCTGCATGCCTGGATCTCCATCCCCTTCTGCTTTGCTTATACTCTGGCCCTGCTAGGCAACTGTACCCT
TCTCTTCATTATCCGGGCTGATGCAGCCCTCCATGAACCCATGTACCTCTTTCTGGCCATGTTGGCAACC
ATTGACTTGGTTCTTTCTTCTACAACGCTGCCCAAAATGCTTGCCATATTCTGGTTCAGGGATCAGGAGA
TCAACTTCTTTGCCTGTCTGGTCCAGATGTTCTTCCTTCACTCCTTCTCCATCATGGAGTCAGCAGTGCT
GCTGGCCATGGCCTTTGACCGCTATGTGGCCATCTGCAAGCCATTGCACTACACGACGGTCCTGACTGGG
TCCCTCATCACCAAGATTGGCATGGCTGCTGTGGCCTGGGCTGTGACACTAATGACTCCACTCCCCTTCC
TGCTCAGACGCTTCCACTACTGCCGAGGCCCAGTGATTGCCCATTGCTACTGTGAACACATGGCTGTGGT
AAGGCTGGCGTGTGGGGACACTAGCTTCAACAATATCTATGGCATTGCTGTGGCCATGTTTATTGTGGTG
TTGGACCTGCTCTTTGTTATCCTGTCTTATGTCTTCATCCTTCAGGCAGTTCTCCAGCTTGCCTCTCAGG
AGGCCCGCTACAAGGCATTTGGGACATGTGTGTCTCACATAGGTGCCATCCTGTCCACCTACACTCCAGT
AGTCATCTCTTCAGTCATGCACCGTGTAGCCCGCCATGCTGCCCCTCGTGTCCACATACTCCTTGCTATT
TTCTATCTCCTTTTCCCACCCATGGTCAATCCTATCATATATGGAGTCAAGACCAAGCAGATTCGTGAGT
ATGTGCTCAGTCTATTCCAGAGAAAGAACATG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG215464 representing NM_001005171
Red=Cloning site Green=Tags(s)

MLPSNITSTHPAVFLLVGIPGLEHLHAWISIPFCFAYTLALLGNCTLLFIIRADAALHEPMYLFLAMLAT
IDLVLSSTTLPKMLAIFWFRDQEINFFACLVQMFFLHSFSIMESAVLLAMAFDRYVAICKPLHYTTVLTG
SLITKIGMAAVAWAVTLMTPLPFLLRRFHYCRGPVIAHCYCEHMAVVRLACGDTSFNNIYGIAVAMFIVV
LDLLFVILSYVFILQAVLQLASQEARYKAFGTCVSHIGAILSTYTPVVISSVMHRVARHAAPRVHILLAI
FYLLFPPMVNPIIYGVKTKQIREYVLSLFQRKNM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001005171
ORF Size 942 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001005171.1, NP_001005171.1
RefSeq Size 945 bp
RefSeq ORF 945 bp
Locus ID 390036
UniProt ID Q8NGK4
Protein Families Transmembrane
Protein Pathways Olfactory transduction
Gene Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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