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Eph receptor A1 (EPHA1) (NM_005232) Human Tagged ORF Clone
CAT＃: RG213689
- TrueORF®
EPHA1 (tGFP-tagged) - Human EPH receptor A1 (EPHA1)
ORF Plasmid: DDK
"NM_005232" in other vectors (8)
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CNY 4,370.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EPH; EPHT; EPHT1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213689 representing NM_005232
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGCGCTGGCCCCTGGGGCTAGGGCTGGTGCTGCTGCTCTGCGCCCCGCTGCCCCCGGGGGCTC ACGCCAAGGAAGTTACTCTGATGGACACAAGCAAGGCACAGGGAGAGCTGGGCTGGCTGCTGGATCCCCC AAAAGATGGGTGGAGTGAACAGCAACAGATACTGAATGGGACACCCCTGTACATGTACCAGGACTGCCCA ATGCAAGGACGCAGAGACACTGACCACTGGCTTCGCTCCAATTGGATCTACCGCGGGGAGGAGGCTTCCC GCGTCCACGTGGAGCTGCAGTTCACCGTGCGGGACTGCAAGAGTTTCCCTGGGGGAGCCGGGCCTCTGGG CTGCAAGGAGACCTTCAACCTTCTGTACATGGAGAGTGACCAGGATGTGGGCATTCAGCTCCGACGGCCC TTGTTCCAGAAGGTAACCACGGTGGCTGCAGACCAGAGCTTCACCATTCGAGACCTTGCGTCTGGCTCCG TGAAGCTGAATGTGGAGCGCTGCTCTCTGGGCCGCCTGACCCGCCGTGGCCTCTACCTCGCTTTCCACAA CCCGGGTGCCTGTGTGGCCCTGGTGTCTGTCCGGGTCTTCTACCAGCGCTGTCCTGAGACCCTGAATGGC TTGGCCCAATTCCCAGACACTCTGCCTGGCCCCGCTGGGTTGGTGGAAGTGGCGGGGACCTGCTTGCCCC ACGCGCGGGCCAGCCCCAGGCCCTCAGGTGCACCCCGCATGCACTGCAGCCCTGATGGCGAGTGGCTGGT GCCTGTAGGACGGTGCCACTGTGAGCCTGGCTATGAGGAAGGTGGCAGTGGCGAAGCATGTGTTGCCTGC CCTAGCGGCTCCTACCGGATGGACATGGACACACCCCATTGTCTCACGTGCCCCCAGCAGAGCACTGCTG AGTCTGAGGGGGCCACCATCTGTACCTGTGAGAGCGGCCATTACAGAGCTCCCGGGGAGGGCCCCCAGGT GGCATGCACAGGTCCCCCCTCGGCCCCCCGAAACCTGAGCTTCTCTGCCTCAGGGACTCAGCTCTCCCTG CGTTGGGAACCCCCAGCAGATACGGGGGGACGCCAGGATGTCAGATACAGTGTGAGGTGTTCCCAGTGTC AGGGCACAGCACAGGACGGGGGGCCCTGCCAGCCCTGTGGGGTGGGCGTGCACTTCTCGCCGGGGGCCCG GGGGCTCACCACACCTGCAGTGCATGTCAATGGCCTTGAACCTTATGCCAACTACACCTTTAATGTGGAA GCCCAAAATGGAGTGTCAGGGCTGGGCAGCTCTGGCCATGCCAGCACCTCAGTCAGCATCAGCATGGGGC ATGCAGAGTCACTGTCAGGCCTGTCTCTGAGACTGGTGAAGAAAGAACCGAGGCAACTAGAGCTGACCTG GGCGGGGTCCCGGCCCCGAAGCCCTGGGGCGAACCTGACCTATGAGCTGCACGTGCTGAACCAGGATGAA GAACGGTACCAGATGGTTCTAGAACCCAGGGTCTTGCTGACAGAGCTGCAGCCTGACACCACATACATCG TCAGAGTCCGAATGCTGACCCCACTGGGTCCTGGCCCTTTCTCCCCTGATCATGAGTTTCGGACCAGCCC ACCAGTGTCCAGGGGCCTGACTGGAGGAGAGATTGTAGCCGTCATCTTTGGGCTGCTGCTTGGTGCAGCC TTGCTGCTTGGGATTCTCGTTTTCCGGTCCAGGAGAGCCCAGCGGCAGAGGCAGCAGAGGCAGCGTGACC GCGCCACCGATGTGGATCGAGAGGACAAGCTGTGGCTGAAGCCTTATGTGGACCTCCAGGCATACGAGGA CCCTGCACAGGGAGCCTTGGACTTTACCCGGGAGCTTGATCCAGCGTGGCTGATGGTGGACACTGTCATA GGAGAAGGAGAGTTTGGGGAAGTGTATCGAGGGACCCTGAGGCTCCCCAGCCAGGACTGCAAGACTGTGG CCATTAAGACCTTAAAAGACACATCCCCAGGTGGCCAGTGGTGGAACTTCCTTCGAGAGGCAACTATCAT GGGCCAGTTTAGCCACCCGCATATTCTGCATCTGGAAGGCGTCGTCACAAAGCGAAAGCCGATCATGATC ATCACAGAATTTATGGAGAATGGAGCCCTGGATGCCTTCCTGAGGGAGCGGGAGGACCAGCTGGTCCCTG GGCAGCTAGTGGCCATGCTGCAGGGCATAGCATCTGGCATGAACTACCTCAGTAATCACAATTATGTCCA CCGGGACCTGGCTGCCAGAAACATCTTGGTGAATCAAAACCTGTGCTGCAAGGTGTCTGACTTTGGCCTG ACTCGCCTCCTGGATGACTTTGATGGCACATACGAAACCCAGGGAGGAAAGATCCCTATCCGTTGGACAG CCCCTGAAGCCATTGCCCATCGGATCTTCACCACAGCCAGCGATGTGTGGAGCTTTGGGATTGTGATGTG GGAGGTGCTGAGCTTTGGGGACAAGCCTTATGGGGAGATGAGCAATCAGGAGGTTATGAAGAGCATTGAG GATGGGTACCGGTTGCCCCCTCCTGTGGACTGCCCTGCCCCTCTGTATGAGCTCATGAAGAACTGCTGGG CATATGACCGTGCCCGCCGGCCACACTTCCAGAAGCTTCAGGCACATCTGGAGCAACTGCTTGCCAACCC CCACTCCCTGCGGACCATTGCCAACTTTGACCCCAGGGTGACTCTTCGCCTGCCCAGCCTGAGTGGCTCA GATGGGATCCCGTATCGAACCGTCTCTGAGTGGCTCGAGTCCATACGCATGAAACGCTACATCCTGCACT TCCACTCGGCTGGGCTGGACACCATGGAGTGTGTGCTGGAGCTGACCGCTGAGGACCTGACGCAGATGGG AATCACACTGCCCGGGCACCAGAAGCGCATTCTTTGCAGTATTCAGGGATTCAAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213689 representing NM_005232
Red=Cloning site Green=Tags(s) MERRWPLGLGLVLLLCAPLPPGAHAKEVTLMDTSKAQGELGWLLDPPKDGWSEQQQILNGTPLYMYQDCP MQGRRDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLLYMESDQDVGIQLRRP LFQKVTTVAADQSFTIRDLASGSVKLNVERCSLGRLTRRGLYLAFHNPGACVALVSVRVFYQRCPETLNG LAQFPDTLPGPAGLVEVAGTCLPHARASPRPSGAPRMHCSPDGEWLVPVGRCHCEPGYEEGGSGEACVAC PSGSYRMDMDTPHCLTCPQQSTAESEGATICTCESGHYRAPGEGPQVACTGPPSAPRNLSFSASGTQLSL RWEPPADTGGRQDVRYSVRCSQCQGTAQDGGPCQPCGVGVHFSPGARGLTTPAVHVNGLEPYANYTFNVE AQNGVSGLGSSGHASTSVSISMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSPGANLTYELHVLNQDE ERYQMVLEPRVLLTELQPDTTYIVRVRMLTPLGPGPFSPDHEFRTSPPVSRGLTGGEIVAVIFGLLLGAA LLLGILVFRSRRAQRQRQQRQRDRATDVDREDKLWLKPYVDLQAYEDPAQGALDFTRELDPAWLMVDTVI GEGEFGEVYRGTLRLPSQDCKTVAIKTLKDTSPGGQWWNFLREATIMGQFSHPHILHLEGVVTKRKPIMI ITEFMENGALDAFLREREDQLVPGQLVAMLQGIASGMNYLSNHNYVHRDLAARNILVNQNLCCKVSDFGL TRLLDDFDGTYETQGGKIPIRWTAPEAIAHRIFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIE DGYRLPPPVDCPAPLYELMKNCWAYDRARRPHFQKLQAHLEQLLANPHSLRTIANFDPRVTLRLPSLSGS DGIPYRTVSEWLESIRMKRYILHFHSAGLDTMECVLELTAEDLTQMGITLPGHQKRILCSIQGFKD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005232 |
| ORF Size | 2928 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005232.5 |
| RefSeq Size | 3367 bp |
| RefSeq ORF | 2931 bp |
| Locus ID | 2041 |
| UniProt ID | P21709 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Axon guidance |
| Gene Summary | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Decreased Eph receptor‑A1 expression is related to grade in ovarian serous carcinoma
,Jin, Y;Zou, Y;Wan, L;Lu, M;Liu, Y;Huang, G;Wang, J;Xi, Q;,
Mol Med Rep
,PubMed ID 29393455
[EPHA1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213689 | EPHA1 (Myc-DDK-tagged)-Human EPH receptor A1 (EPHA1) |
CNY 3,990.00 |
|
| RC213689L1 | Lenti ORF clone of Human EPH receptor A1 (EPHA1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213689L2 | Lenti ORF clone of Human EPH receptor A1 (EPHA1), mGFP tagged |
CNY 5,890.00 |
|
| RC213689L3 | Lenti ORF clone of Human EPH receptor A1 (EPHA1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213689L4 | Lenti ORF clone of Human EPH receptor A1 (EPHA1), mGFP tagged |
CNY 5,890.00 |
|
| RC600037 | EPHA1 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor A1, Signal peptide (1-25) plus EC domain (26-547) |
CNY 4,072.00 |
|
| SC116861 | EPHA1 (untagged)-Human EPH receptor A1 (EPHA1) |
CNY 7,152.00 |
|
| SC323534 | EPHA1 (untagged)-Kinase deficient mutant (K656M) of Human EPH receptor A1 (EPHA1) |
CNY 7,152.00 |
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