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Eph receptor A5 (EPHA5) (NM_004439) Human Tagged ORF Clone
CAT＃: RG213206
- TrueORF®
EPHA5 (tGFP-tagged) - Human EPH receptor A5 (EPHA5), transcript variant 1
ORF Plasmid: DDK
"NM_004439" in other vectors (8)
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CNY 12,432.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CEK7; EHK-1; EHK1; EK7; HEK7; TYRO4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213206 representing NM_004439.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGGGGCTCGGGGCCCCGGGGTGCGGGACGCCGGCGGCCCCCAAGCGGCGGCGGCGACACCCCCATC ACCCCAGCGTCCCTGGCCGGCTGCTACTCTGCACCTCGACGGGCTCCCCTCTGGACGTGCCTTCTCCTG TGCGCCGCACTCCGGACCCTCCTGGCCAGCCCCAGCAACGAAGTGAATTTATTGGATTCACGCACTGTC ATGGGGGACCTGGGATGGATTGCTTTTCCAAAAAATGGGTGGGAAGAGATTGGTGAAGTGGATGAAAAT TATGCCCCTATCCACACATACCAAGTATGCAAAGTGATGGAACAGAATCAGAATAACTGGCTTTTGACC AGTTGGATCTCCAATGAAGGTGCTTCCAGAATCTTCATAGAACTCAAATTTACCCTGCGGGACTGCAAC AGCCTTCCTGGAGGACTGGGGACCTGTAAGGAAACCTTTAATATGTATTACTTTGAGTCAGATGATCAG AATGGGAGAAACATCAAGGAAAACCAATACATCAAAATTGATACCATTGCTGCCGATGAAAGCTTTACA GAACTTGATCTTGGTGACCGTGTTATGAAACTGAATACAGAGGTCAGAGATGTAGGACCTCTAAGCAAA AAGGGATTTTATCTTGCTTTTCAAGATGTTGGTGCTTGCATTGCTCTGGTTTCTGTGCGTGTATACTAT AAAAAATGCCCTTCTGTGGTACGACACTTGGCTGTCTTCCCTGACACCATCACTGGAGCTGATTCTTCC CAATTGCTCGAAGTGTCAGGCTCCTGTGTCAACCATTCTGTGACCGATGAACCTCCCAAAATGCACTGC AGCGCCGAAGGGGAGTGGCTGGTGCCCATCGGGAAATGCATGTGCAAGGCAGGATATGAAGAGAAAAAT GGCACCTGTCAAGTGTGCAGACCTGGGTTCTTCAAAGCCTCACCTCACATCCAGAGCTGCGGCAAATGT CCACCTCACAGTTATACCCATGAGGAAGCTTCAACCTCTTGTGTCTGTGAAAAGGATTATTTCAGGAGA GAGTCTGATCCACCCACAATGGCATGCACAAGACCCCCCTCTGCTCCTCGGAATGCCATCTCAAATGTT AATGAAACTAGTGTCTTTCTGGAATGGATTCCGCCTGCTGACACTGGTGGAAGGAAAGACGTGTCATAT TATATTGCATGCAAGAAGTGCAACTCCCATGCAGGTGTGTGTGAGGAGTGTGGCGGTCATGTCAGGTAC CTTCCCCGGCAAAGCGGCCTGAAAAACACCTCTGTCATGATGGTGGATCTACTCGCTCACACAAACTAT ACCTTTGAGATTGAGGCAGTGAATGGAGTGTCCGACTTGAGCCCAGGAGCCCGGCAGTATGTGTCTGTA AATGTAACCACAAATCAAGCAGCTCCATCTCCAGTCACCAATGTGAAAAAAGGGAAAATTGCAAAAAAC AGCATCTCTTTGTCTTGGCAAGAACCAGATCGTCCCAATGGAATCATCCTAGAGTATGAAATCAAGTAT TTTGAAAAGGACCAAGAGACCAGCTACACGATTATCAAATCTAAAGAGACAACTATTACTGCAGAGGGC TTGAAACCAGCTTCAGTTTATGTCTTCCAAATTCGAGCACGTACAGCAGCAGGCTATGGTGTCTTCAGT CGAAGATTTGAGTTTGAAACCACCCCAGTGTTTGCAGCATCCAGCGATCAAAGCCAGATTCCTGTAATT GCTGTGTCTGTGACAGTGGGAGTCATTTTGTTGGCAGTGGTTATCGGCGTCCTCCTCAGTGGAAGTTGC TGCGAATGTGGCTGTGGGAGGGCTTCTTCCCTGTGCGCTGTTGCCCATCCAAGCCTAATATGGCGGTGT GGCTACAGCAAAGCAAAACAAGATCCAGAAGAGGAAAAGATGCATTTTCATAATGGGCACATTAAACTG CCAGGAGTAAGAACTTACATTGATCCACATACCTATGAGGATCCCAATCAAGCTGTCCACGAATTTGCT AAGGAGATAGAAGCATCATGTATCACCATTGAGAGAGTTATTGGAGCAGGTGAATTTGGTGAAGTTTGT AGTGGACGTTTGAAACTACCAGGAAAAAGAGAATTACCTGTGGCTATCAAAACCCTTAAAGTAGGCTAT ACTGAAAAGCAACGCAGAGATTTCCTAGGTGAAGCAAGTATCATGGGACAGTTTGATCATCCTAACATC ATCCATTTAGAAGGTGTGGTGACCAAAAGTAAACCAGTGATGATCGTGACAGAGTATATGGAGAATGGC TCTTTAGATACATTTTTGAAGAAAAACGATGGGCAGTTCACTGTGATTCAGCTTGTTGGCATGCTGAGA GGTATCTCTGCAGGAATGAAGTACCTTTCTGACATGGGCTATGTGCATAGAGATCTTGCTGCCAGAAAC ATCTTAATCAACAGTAACCTTGTGTGCAAAGTGTCTGACTTTGGACTTTCCCGGGTACTGGAAGATGAT CCCGAGGCAGCCTACACCACAAGGGGAGGAAAAATTCCAATCAGATGGACTGCCCCAGAAGCAATAGCT TTCCGAAAGTTTACTTCTGCCAGTGATGTCTGGAGTTATGGAATAGTAATGTGGGAAGTTGTGTCTTAT GGAGAGAGACCCTACTGGGAGATGACCAATCAAGATGTGATTAAAGCGGTAGAGGAAGGCTATCGTCTG CCAAGCCCCATGGATTGTCCTGCTGCTCTCTATCAGTTAATGCTGGATTGCTGGCAGAAAGAGCGAAAT AGCAGGCCCAAGTTTGATGAAATAGTCAACATGTTGGACAAGCTGATACGTAACCCAAGTAGTCTGAAG ACGCTGGTTAATGCATCCTGCAGAGTATCTAATTTATTGGCAGAACATAGCCCACTAGGATCTGGAGCC TACAGATCAGTAGGTGAATGGCTAGAGGCAATCAAGATGGGCCGGTATACAGAGATTTTCATGGAAAAT GGATACAGTTCAATGGACGCTGTGGCTCAGGTGACCTTGGAGGATTTGAGACGGCTTGGAGTGACTCTT GTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGAAATGAAGGTGCAGCTGGTAAACGGAATGGTG CCATTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG213206
Blue=ORF Red=Cloning site Green=Tag(s) MRGSGPRGAGRRRPPSGGGDTPITPASLAGCYSAPRRAPLWTCLLLCAALRTLLASPSNEVNLLDSRTV MGDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCN SLPGGLGTCKETFNMYYFESDDQNGRNIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSK KGFYLAFQDVGACIALVSVRVYYKKCPSVVRHLAVFPDTITGADSSQLLEVSGSCVNHSVTDEPPKMHC SAEGEWLVPIGKCMCKAGYEEKNGTCQVCRPGFFKASPHIQSCGKCPPHSYTHEEASTSCVCEKDYFRR ESDPPTMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGRKDVSYYIACKKCNSHAGVCEECGGHVRY LPRQSGLKNTSVMMVDLLAHTNYTFEIEAVNGVSDLSPGARQYVSVNVTTNQAAPSPVTNVKKGKIAKN SISLSWQEPDRPNGIILEYEIKYFEKDQETSYTIIKSKETTITAEGLKPASVYVFQIRARTAAGYGVFS RRFEFETTPVFAASSDQSQIPVIAVSVTVGVILLAVVIGVLLSGSCCECGCGRASSLCAVAHPSLIWRC GYSKAKQDPEEEKMHFHNGHIKLPGVRTYIDPHTYEDPNQAVHEFAKEIEASCITIERVIGAGEFGEVC SGRLKLPGKRELPVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIHLEGVVTKSKPVMIVTEYMENG SLDTFLKKNDGQFTVIQLVGMLRGISAGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDD PEAAYTTRGGKIPIRWTAPEAIAFRKFTSASDVWSYGIVMWEVVSYGERPYWEMTNQDVIKAVEEGYRL PSPMDCPAALYQLMLDCWQKERNSRPKFDEIVNMLDKLIRNPSSLKTLVNASCRVSNLLAEHSPLGSGA YRSVGEWLEAIKMGRYTEIFMENGYSSMDAVAQVTLEDLRRLGVTLVGHQKKIMNSLQEMKVQLVNGMV PL TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004439 |
| ORF Size | 3111 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 3349 bp |
| RefSeq ORF | 3114 bp |
| Locus ID | 2044 |
| UniProt ID | P54756 |
| Domains | pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Axon guidance |
| MW | 114.8 kDa |
| Gene Summary | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Receptor Tyrosine Kinase EphA5 is a Functional Molecular Target in Human Lung Cancer
,Staquicini, FI;Qian, MD;Salameh, A;Dobroff, AS;Edwards, JK;Cimino, DF;Moeller, BJ;Kelly, P;Nunez, MI;Tang, X;Liu, DD;Lee, JJ;Hong, WK;Ferrara, F;Bradbury, AR;Lobb, RR;Edelman, MJ;Sidman, RL;Wistuba, II;Arap, W;Pasqualini, R;,
J. Biol. Chem.
,PubMed ID 25623065
[EPHA5]
|
|
Downregulation of EphA5 by promoter methylation in human prostate cancer
,null,
BMC Cancer
,PubMed ID 25609195
[EPHA5]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213206 | EPHA5 (Myc-DDK-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 10,832.00 |
|
| RC213206L1 | Lenti-ORF clone of EPHA5 (Myc-DDK-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 13,232.00 |
|
| RC213206L2 | Lenti-ORF clone of EPHA5 (mGFP-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 7,600.00 |
|
| RC213206L3 | Lenti-ORF clone of EPHA5 (Myc-DDK-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 7,600.00 |
|
| RC213206L4 | Lenti-ORF clone of EPHA5 (mGFP-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 7,600.00 |
|
| RC600042 | EPHA5 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor A5, transcript variant 1, Signal peptide (1-24) plus EC domain (25-573) |
CNY 4,264.00 |
|
| SC309998 | EPHA5 (untagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 10,840.00 |
|
| SC323514 | EPHA5 (untagged)-Kinase deficient mutant (K685M) of Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 10,840.00 |
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