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TAF6 (NM_005641) Human Tagged ORF Clone
CAT＃: RG213036
- TrueORF®
TAF6 (tGFP-tagged) - Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1
ORF Plasmid: DDK
"NM_005641" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ALYUS; MGC:8964; TAF(II)70; TAF(II)80; TAF2E; TAFII-70; TAFII-80; TAFII70; TAFII80; TAFII85 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213036 representing NM_005641
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGGAGAAGAAGCTGAAGCTTAGCAACACTGTGCTGCCCTCGGAGTCCATGAAGGTGGTGGCTG AATCCATGGGCATCGCCCAGATTCAGGAGGAGACCTGCCAGCTGCTAACGGATGAGGTCAGCTACCGCAT CAAAGAGATCGCACAGGATGCCTTGAAGTTCATGCACATGGGGAAGCGGCAGAAGCTCACCACCAGTGAC ATTGACTACGCCTTGAAGCTAAAGAATGTCGAGCCACTCTATGGCTTCCACGCCCAGGAGTTCATTCCTT TCCGCTTCGCCTCTGGTGGGGGCCGGGAGCTTTACTTCTATGAGGAGAAGGAGGTTGATCTGAGCGACAT CATCAATACCCCTCTGCCCCGGGTGCCCCTGGACGTCTGCCTCAAAGCTCATTGGCTGAGCATCGAGGGC TGCCAGCCAGCTATCCCCGAGAACCCGCCCCCAGCTCCCAAAGAGCAACAGAAGGCTGAAGCCACAGAAC CCCTGAAGTCAGCCAAGCCAGGCCAGGAGGAAGACGGACCCCTGAAGGGCAAAGGTCAAGGGGCCACCAC AGCCGACGGCAAAGGGAAAGAGAAGAAGGCGCCGCCCTTGCTGGAGGGGGCCCCCTTGCGACTGAAGCCC CGGAGCATCCACGAGTTGTCTGTGGAGCAGCAGCTCTACTACAAGGAGATCACCGAGGCCTGCGTGGGCT CCTGCGAGGCCAAGAGGGCGGAAGCCCTGCAAAGCATTGCCACGGACCCTGGACTGTATCAGATGCTGCC ACGGTTCAGTACCTTTATCTCGGAGGGGGTCCGTGTGAACGTGGTTCAGAACAACCTGGCCCTACTCATC TACCTGATGCGTATGGTGAAAGCGCTGATGGACAACCCCACGCTCTATCTAGAAAAATACGTCCATGAGC TGATTCCAGCTGTGATGACCTGCATCGTGAGCAGACAGTTGTGCCTGCGACCAGATGTGGACAATCACTG GGCACTCCGAGACTTTGCTGCCCGCCTGGTGGCCCAGATCTGCAAGCATTTTAGCACAACCACTAACAAC ATCCAGTCCCGGATCACCAAGACCTTCACCAAGAGCTGGGTGGACGAGAAGACGCCCTGGACGACTCGTT ATGGCTCCATCGCAGGCTTGGCTGAGCTGGGACACGATGTTATCAAGACTCTGATTCTGCCCCGGCTGCA GCAGGAAGGGGAGCGGATCCGCAGTGTGCTGGACGGCCCTGTGCTGAGCAACATTGACCGGATTGGAGCA GACCATGTGCAGAGCCTCCTGCTGAAACACTGTGCTCCTGTTCTGGCAAAGCTGCGCCCACCGCCTGACA ATCAGGACGCCTATCGGGCAGAATTCGGGTCCCTTGGGCCCCTCCTCTGCTCCCAGGTGGTCAAGGCTCG GGCCCAGGCTGCTCTGCAGGCTCAGCAGGTCAACAGGACCACTCTGACCATCACGCAGCCCCGGCCCACG CTGACCCTCTCGCAGGCCCCACAGCCTGGCCCTCGCACCCCTGGCTTGCTGAAGGTTCCTGGCTCCATCG CACTTCCTGTCCAGACACTGGTGTCTGCACGAGCGGCTGCCCCACCACAGCCTTCCCCTCCTCCAACCAA GTTTATTGTAATGTCATCGTCCTCCAGCGCCCCATCCACCCAGCAGGTCCTGTCCCTCAGCACCTCGGCC CCCGGCTCAGGTTCCACCACCACTTCGCCCGTCACCACCACCGTCCCCAGCGTGCAGCCCATCGTCAAGT TGGTCTCCACCGCCACCACCGCACCCCCCAGCACTGCTCCCTCTGGTCCTGGGAGTGTCCAGAAGTACAT CGTGGTCTCACTTCCCCCAACAGGGGAGGGCAAAGGAGGCCCCACCTCCCATCCTTCTCCAGTTCCTCCC CCGGCATCGTCCCCGTCCCCACTCAGCGGCAGTGCCCTTTGTGGGGGGAAGCAGGAGGCTGGGGACAGTC CCCCTCCAGCTCCAGGGACTCCAAAAGCCAATGGCTCCCAGCCCAACTCCGGCTCCCCTCAGCCTGCTCC G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213036 representing NM_005641
Red=Cloning site Green=Tags(s) MAEEKKLKLSNTVLPSESMKVVAESMGIAQIQEETCQLLTDEVSYRIKEIAQDALKFMHMGKRQKLTTSD IDYALKLKNVEPLYGFHAQEFIPFRFASGGGRELYFYEEKEVDLSDIINTPLPRVPLDVCLKAHWLSIEG CQPAIPENPPPAPKEQQKAEATEPLKSAKPGQEEDGPLKGKGQGATTADGKGKEKKAPPLLEGAPLRLKP RSIHELSVEQQLYYKEITEACVGSCEAKRAEALQSIATDPGLYQMLPRFSTFISEGVRVNVVQNNLALLI YLMRMVKALMDNPTLYLEKYVHELIPAVMTCIVSRQLCLRPDVDNHWALRDFAARLVAQICKHFSTTTNN IQSRITKTFTKSWVDEKTPWTTRYGSIAGLAELGHDVIKTLILPRLQQEGERIRSVLDGPVLSNIDRIGA DHVQSLLLKHCAPVLAKLRPPPDNQDAYRAEFGSLGPLLCSQVVKARAQAALQAQQVNRTTLTITQPRPT LTLSQAPQPGPRTPGLLKVPGSIALPVQTLVSARAAAPPQPSPPPTKFIVMSSSSSAPSTQQVLSLSTSA PGSGSTTTSPVTTTVPSVQPIVKLVSTATTAPPSTAPSGPGSVQKYIVVSLPPTGEGKGGPTSHPSPVPP PASSPSPLSGSALCGGKQEAGDSPPPAPGTPKANGSQPNSGSPQPAP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005641 |
| ORF Size | 2784 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005641.4 |
| RefSeq Size | 2737 bp |
| RefSeq ORF | 2034 bp |
| Locus ID | 6878 |
| UniProt ID | P49848 |
| Domains | TAF |
| Protein Families | Transcription Factors |
| Protein Pathways | Basal transcription factors |
| Gene Summary | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213036 | TAF6 (Myc-DDK-tagged)-Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1 |
CNY 3,990.00 |
|
| RC213036L3 | Lenti-ORF clone of TAF6 (Myc-DDK-tagged)-Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1 |
CNY 5,890.00 |
|
| RC213036L4 | Lenti-ORF clone of TAF6 (mGFP-tagged)-Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1 |
CNY 5,890.00 |
|
| SC111695 | TAF6 (untagged)-Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1 |
CNY 2,950.00 |
