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AP3B2 (NM_004644) Human Tagged ORF Clone
CAT＃: RG210820
- TrueORF®
AP3B2 (tGFP-tagged) - Human adaptor-related protein complex 3, beta 2 subunit (AP3B2)
ORF Plasmid: DDK
"NM_004644" in other vectors (6)
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CNY 9,136.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DEE48; EIEE48; NAPTB |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210820 representing NM_004644
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCGCCCCCGCCTACAGCGAAGACAAGGGCGGCTCCGCTGGCCCCGGGGAGCCCGAGTACGGCC ACGACCCCGCGAGCGGCGGCATCTTCTCCTCCGACTACAAGCGGCATGATGACCTGAAGGAGATGCTGGA CACCAACAAGGATTCTCTCAAGCTGGAGGCCATGAAGAGGATTGTGGCGATGATTGCCCGAGGAAAGAAT GCTTCAGACCTGTTTCCCGCGGTGGTGAAGAACGTGGCCTGTAAGAACATAGAGGTGAAGAAGCTTGTCT ATGTGTACCTGGTACGCTACGCTGAGGAGCAGCAAGACCTGGCCCTGCTGTCCATCTCCACCTTCCAACG TGGCCTAAAGGATCCCAACCAGCTGATTCGTGCCAGTGCCCTCCGTGTCCTCTCTAGCATCCGTGTGCCC ATCATAGTGCCCATCATGATGCTAGCTATCAAGGAAGCCGCCTCGGACATGTCACCCTATGTGCGGAAAA CAGCTGCCCACGCCATCCCTAAACTCTACAGTTTGGACTCTGACCAGAAGGATCAGCTGATAGAAGTCAT TGAGAAGCTTCTGGCTGACAAGACCACGCTGGTGGCGGGCAGTGTGGTGATGGCCTTTGAGGAGGTCTGC CCGGAGCGCATCGACCTGATTCACAAAAACTACCGGAAACTCTGTAACCTGCTGATCGACGTGGAGGAGT GGGGCCAGGTGGTCATCATCAGCATGCTCACCCGCTACGCCCGCACGCAGTTCCTGAGCCCCACCCAGAA CGAATCCCTACTAGAGGAGAACGCGGAAAAAGCCTTCTACGGCTCAGAGGAGGACGAGGCCAAGGGCGCG GGGTCTGAGGAGACGGCCGCCGCGGCCGCCCCCTCCCGAAAGCCCTATGTCATGGACCCCGACCACCGGC TGCTGCTGCGCAACACCAAACCCCTGCTGCAGAGCCGCAGCGCCGCGGTGGTGATGGCGGTGGCGCAGCT CTACTTCCACCTGGCGCCCAAGGCGGAAGTGGGCGTCATCGCCAAGGCGCTGGTGCGCCTGCTGCGCAGC CACAGTGAGGTGCAGTACGTTGTGCTCCAGAACGTGGCCACCATGTCCATCAAGCGCCGGGGTATGTTTG AGCCCTACCTGAAGAGCTTCTACATCAGGTCCACCGACCCCACCCAGATTAAGATCCTGAAGCTGGAAGT GCTGACCAACCTGGCCAATGAGACCAACATTCCTACTGTCCTACGGGAATTCCAGACCTATATTCGCAGC ATGGACAAGGACTTTGTGGCAGCCACAATCCAGGCCATTGGACGCTGTGCAACTAACATCGGCCGAGTCC GTGACACCTGCCTCAATGGCCTGGTGCAGCTGCTGTCCAACCGTGATGAGCTTGTGGTTGCAGAGTCAGT GGTCGTCATTAAGAAATTGCTACAGATGCAGCCAGCACAACATGGAGAGATCATCAAACACTTGGCAAAG CTTACAGACAACATCCAGGTGCCCATGGCCCGAGCCAGCATCCTGTGGCTCATCGGAGAGTACTGTGAGC ATGTCCCCAGGATTGCACCTGATGTCTTAAGAAAAATGGCCAAGTCATTCACAGCAGAGGAGGATATTGT CAAGCTGCAGGTCATCAACCTGGCAGCCAAGCTCTACCTGACCAACTCTAAACAGACCAAGCTGCTGACC CAGTATGTGCTGAGTCTGGCCAAATATGACCAGAACTATGATATTCGCGACCGGGCGCGCTTCACCCGGC AGCTCATCGTCCCTTCCGAGCAGGGTGGGGCCCTCAGCCGCCATGCCAAGAAGCTCTTCCTGGCACCCAA ACCAGCTCCAGTCTTGGAGTCATCCTTCAAAGACCGGGACCACTTCCAGCTGGGCTCACTGTCCCACCTG CTTAATGCCAAGGCCACAGGCTACCAGGAGCTCCCAGACTGGCCGGAGGAAGCCCCAGACCCATCTGTGC GCAACGTGGAGGTACCTGAATGGACCAAGTGCTCAAATCGGGAGAAGAGAAAGGAGAAGGAAAAACCCTT CTACTCGGACTCTGAGGGGGAGTCAGGCCCCACGGAGTCCGCAGACAGTGACCCTGAGTCTGAGAGTGAA TCGGACAGTAAGAGCAGCAGTGAGAGCGGCTCTGGGGAGTCCAGCAGTGAGTCCGACAATGAAGACCAGG ATGAGGATGAGGAGAAAGGGAGAGGCAGTGAGAGTGAACAGAGTGAGGAGGATGGTAAGAGGAAGACAAA GAAGAAGGTGCCAGAGAGAAAAGGAGAAGCGTCATCCTCTGATGAGGGCAGCGATTCCAGCAGTAGCTCA TCAGAGTCCGAGATGACATCGGAGTCCGAGGAGGAGCAGTTAGAACCTGCCTCCTGGAGCAGGAAAACAC CTCCCAGCAGCAAAAGTGCTCCTGCAACCAAGGAGATCTCCCTGCTTGATCTAGAGGATTTCACCCCTCC CAGTGTCCAGCCTGTGTCTCCCCCAGCAATTGTGTCTACCAGTCTGGCTGCTGACCTGGAGGGCCTGACA CTCACAGACTCCACCCTGGTACCGTCGCTTCTGAGTCCAGTATCGGGTGTTGGGCGGCAGGAGCTGCTGC ACCGGGTAGCTGGCGAGGGGCTGGCTGTGGACTACACCTTCAGCCGCCAACCTTTCTCCGGGGATCCCCA CATGGTGTCCGTGCACATCCACTTCTCCAACAGCTCTGATACCCCCATCAAGGGCCTGCATGTGGGCACT CCCAAACTGCCTGCTGGCATCAGCATCCAAGAATTTCCCGAAATTGAGTCCCTGGCACCTGGAGAATCTG CCACTGCTGTAATGGGCATTAATTTCTGTGACTCAACCCAGGCAGCCAACTTCCAGCTGTGCACCCAGAC CCGACAGTTCTACGTCTCCATTCAGCCACCTGTTGGGGAGCTGATGGCCCCTGTGTTCATGAGTGAAAAT GAGTTTAAGAAGGAACAGGGAAAGCTGATGGGCATGAATGAGATCACAGAGAAACTCATGCTGCCAGACA CCTGTCGGAGTGACCACATTGTGGTGCAGAAAGTGACTGCCACTGCCAACCTGGGTCGTGTTCCTTGTGG GACATCTGATGAGTACAGGTTTGCAGGGAGGACACTGACTGGTGGAAGCCTCGTTCTGCTGACCCTGGAT GCCCGGCCAGCTGGAGCTGCCCAGCTGACTGTCAACAGCGAGAAAATGGTGATTGGCACCATGCTGGTAA AGGATGTGATACAGGCTCTGACCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210820 representing NM_004644
Red=Cloning site Green=Tags(s) MSAAPAYSEDKGGSAGPGEPEYGHDPASGGIFSSDYKRHDDLKEMLDTNKDSLKLEAMKRIVAMIARGKN ASDLFPAVVKNVACKNIEVKKLVYVYLVRYAEEQQDLALLSISTFQRGLKDPNQLIRASALRVLSSIRVP IIVPIMMLAIKEAASDMSPYVRKTAAHAIPKLYSLDSDQKDQLIEVIEKLLADKTTLVAGSVVMAFEEVC PERIDLIHKNYRKLCNLLIDVEEWGQVVIISMLTRYARTQFLSPTQNESLLEENAEKAFYGSEEDEAKGA GSEETAAAAAPSRKPYVMDPDHRLLLRNTKPLLQSRSAAVVMAVAQLYFHLAPKAEVGVIAKALVRLLRS HSEVQYVVLQNVATMSIKRRGMFEPYLKSFYIRSTDPTQIKILKLEVLTNLANETNIPTVLREFQTYIRS MDKDFVAATIQAIGRCATNIGRVRDTCLNGLVQLLSNRDELVVAESVVVIKKLLQMQPAQHGEIIKHLAK LTDNIQVPMARASILWLIGEYCEHVPRIAPDVLRKMAKSFTAEEDIVKLQVINLAAKLYLTNSKQTKLLT QYVLSLAKYDQNYDIRDRARFTRQLIVPSEQGGALSRHAKKLFLAPKPAPVLESSFKDRDHFQLGSLSHL LNAKATGYQELPDWPEEAPDPSVRNVEVPEWTKCSNREKRKEKEKPFYSDSEGESGPTESADSDPESESE SDSKSSSESGSGESSSESDNEDQDEDEEKGRGSESEQSEEDGKRKTKKKVPERKGEASSSDEGSDSSSSS SESEMTSESEEEQLEPASWSRKTPPSSKSAPATKEISLLDLEDFTPPSVQPVSPPAIVSTSLAADLEGLT LTDSTLVPSLLSPVSGVGRQELLHRVAGEGLAVDYTFSRQPFSGDPHMVSVHIHFSNSSDTPIKGLHVGT PKLPAGISIQEFPEIESLAPGESATAVMGINFCDSTQAANFQLCTQTRQFYVSIQPPVGELMAPVFMSEN EFKKEQGKLMGMNEITEKLMLPDTCRSDHIVVQKVTATANLGRVPCGTSDEYRFAGRTLTGGSLVLLTLD ARPAGAAQLTVNSEKMVIGTMLVKDVIQALTQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004644 |
| ORF Size | 3246 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004644.5 |
| RefSeq Size | 3746 bp |
| RefSeq ORF | 3249 bp |
| Locus ID | 8120 |
| UniProt ID | Q13367 |
| Domains | Adaptin_N |
| Protein Pathways | Lysosome |
| Gene Summary | Adaptor protein complex 3 (AP-3 complex) is a heterotrimeric protein complex involved in the formation of clathrin-coated synaptic vesicles. The protein encoded by this gene represents the beta subunit of the neuron-specific AP-3 complex and was first identified as the target antigen in human paraneoplastic neurologic disorders. The encoded subunit binds clathrin and is phosphorylated by a casein kinase-like protein, which mediates synaptic vesicle coat assembly. Defects in this gene are a cause of early-onset epileptic encephalopathy. [provided by RefSeq, Feb 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210820 | AP3B2 (Myc-DDK-tagged)-Human adaptor-related protein complex 3, beta 2 subunit (AP3B2) |
CNY 7,536.00 |
|
| RC210820L1 | Lenti ORF clone of Human adaptor-related protein complex 3, beta 2 subunit (AP3B2), Myc-DDK-tagged |
CNY 9,936.00 |
|
| RC210820L2 | Lenti ORF clone of Human adaptor-related protein complex 3, beta 2 subunit (AP3B2), mGFP tagged |
CNY 12,730.00 |
|
| RC210820L3 | Lenti ORF clone of Human adaptor-related protein complex 3, beta 2 subunit (AP3B2), Myc-DDK-tagged |
CNY 12,730.00 |
|
| RC210820L4 | Lenti ORF clone of Human adaptor-related protein complex 3, beta 2 subunit (AP3B2), mGFP tagged |
CNY 12,730.00 |
|
| SC117246 | AP3B2 (untagged)-Human adaptor-related protein complex 3, beta 2 subunit (AP3B2) |
CNY 7,544.00 |
