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SEMA4G (NM_017893) Human Tagged ORF Clone
CAT＃: RG210343
- TrueORF®
SEMA4G (tGFP-tagged) - Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (SEMA4G), transcript variant 1
ORF Plasmid: DDK
"NM_017893" in other vectors (5)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210343 representing NM_017893
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGGGAGGCTCTGGCCCCTCCTCCTCAGCATCCTCACAGCAACTGCAGTCCCAGGACCCTCACTGC GGAGACCGTCTAGAGAACTAGATGCCACCCCTCGGATGACCATACCCTATGAAGAGCTCTCTGGGACCCG GCACTTCAAGGGCCAAGCCCAGAACTACTCAACACTGCTGCTGGAGGAGGCCTCAGCAAGGCTGCTGGTG GGAGCCCGAGGTGCCCTGTTCTCTCTCAGTGCCAACGACATAGGAGATGGGGCTCACAAAGAGATCCACT GGGAAGCCTCCCCAGAGATGCAAAGCAAATGTCATCAAAAAGGGAAAAACAACCAGACGGAGTGCTTTAA CCATGTGCGGTTCCTGCAGCGGCTCAATTCTACCCACCTCTATGCATGTGGGACTCACGCCTTCCAGCCC CTCTGTGCAGCCATTGATGCTGAGGCCTTCACCTTGCCAACCAGCTTCGAGGAGGGGAAGGAGAAGTGTC CTTATGACCCAGCCCGTGGCTTCACAGGCCTCATCATTGATGGAGGCCTCTACACAGCCACTAGGTATGA ATTCCGGAGCATTCCTGACATCCGCCGGAGCCGCCACCCACACTCCCTGAGAACTGAGGAGACACCAATG CATTGGCTCAATGATGCGGAGTTTGTGTTCTCCGTCCTCGTGCGGGAGAGCAAGGCCAGTGCAGTGGGTG ATGATGACAAGGTGTACTACTTCTTCACGGAGCGTGCCACTGAGGAGGGCTCTGGCAGCTTCACTCAGAG CCGCAGCAGTCACCGTGTGGCCCGTGTGGCTCGTGTCTGCAAGGGAGACCTGGGAGGGAAGAAGATCCTG CAGAAGAAGTGGACTTCCTTCCTGAAAGCCCGTCTCATCTGCCACATTCCACTGTATGAGACACTGCGTG GGGTCTGCAGCCTGGATGCTGAAACCTCAAGCCGTACACACTTCTATGCAGCCTTCACGCTGAGCACACA GTGGAAGACCCTGGAGGCCTCAGCCATCTGCCGCTATGACCTGGCAGAGATCCAGGCTGTCTTTGCAGGA CCCTATATGGAATACCAGGATGGTTCCCGGCGCTGGGGTCGCTATGAGGGTGGGGTGCCTGAGCCCCGGC CTGGCTCGTGTATCACAGATTCATTGCGCAGCCAAGGCTACAATTCATCCCAAGACTTGCCATCCCTGGT CCTGGACTTTGTAAAGTTGCACCCACTGATGGCTCGGCCCGTTGTGCCCACACGTGGACGGCCCCTGCTG CTCAAGCGCAACATACGCTACACACACCTTACAGGGACACCTGTCACCACGCCTGCTGGACCTACCTATG ACCTGCTCTTTCTGGGCACAGCTGATGGCTGGATCCACAAGGCCGTAGTCCTGGGCTCTGGGATGCACAT TATTGAAGAGACACAAGTGTTCAGGGAGTCCCAGTCTGTGGAAAATCTAGTCATCTCTCTATTGCAGCAC AGCCTCTATGTGGGGGCTCCTAGCGGAGTCATCCAGCTACCACTCTCCAGCTGCTCCCGCTACCGATCCT GCTATGACTGCATCTTGGCCCGAGACCCCTACTGTGGCTGGGACCCTGGCACCCATGCCTGCGCAGCAGC CACCACCATAGCCAACAGGTCCCAGGGAAGCAGGACAGCACTGATACAGGACATAGAGAGAGGAAATCGA GGCTGTGAGAGCAGCAGGGATACAGGGCCACCACCACCACTGAAGACCCGCTCTGTGCTCCGGGGTGATG ATGTCCTCCTGCCCTGTGACCAGCCATCCAACCTGGCCCGGGCCTTGTGGCTACTCAATGGGAGCATGGG CCTGAGCGATGGGCAGGGTGGCTACCGTGTGGGCGTGGACGGGCTGCTGGTTACAGATGCACAGCCTGAG CACAGTGGCAACTATGGCTGCTATGCCGAGGAAAATGGCCTCCGCACCCTGCTGGCCTCCTATAGTCTCA CAGTCCGGCCAGCCACTCCTGCCCCAGCTCCAAAAGCCCCTGCCACACCTGGGGCACAGCTGGCACCTGA TGTGAGACTGCTCTATGTGCTAGCCATTGCCGCGCTTGGTGGCCTCTGCCTCATCCTGGCCTCCTCCCTC CTCTATGTGGCCTGTCTGCGGGAAGGCAGACGAGGGCGCCGACGGAAATACTCACTGGGTCGGGCCAGCC GGGCAGGAGGATCTGCGGTGCAACTGCAGACAGTCTCAGGCCAGTGTCCTGGAGAGGAAGATGAGGGTGA TGATGAGGGGGCTGGGGGCCTGGAGGGCAGCTGTCTCCAGATCATCCCTGGGGAGGGAGCCCCAGCCCCA CCACCCCCACCGCCCCCACCGCCACCGGCTGAGCTGACCAATGGCTTGGTGGCACTGCCCAGCCGGCTGC GGAGGATGAATGGCAATAGCTATGTGCTTCTGAGGCAGAGCAACAATGGAGTACCAGCAGGGCCCTGCTC CTTCGCCGAGGAACTCAGCCGCATCCTGGAAAAAAGGAAGCACACGCAGCTCGTGGAGCAGCTAGATGAG AGCTCTGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210343 representing NM_017893
Red=Cloning site Green=Tags(s) MWGRLWPLLLSILTATAVPGPSLRRPSRELDATPRMTIPYEELSGTRHFKGQAQNYSTLLLEEASARLLV GARGALFSLSANDIGDGAHKEIHWEASPEMQSKCHQKGKNNQTECFNHVRFLQRLNSTHLYACGTHAFQP LCAAIDAEAFTLPTSFEEGKEKCPYDPARGFTGLIIDGGLYTATRYEFRSIPDIRRSRHPHSLRTEETPM HWLNDAEFVFSVLVRESKASAVGDDDKVYYFFTERATEEGSGSFTQSRSSHRVARVARVCKGDLGGKKIL QKKWTSFLKARLICHIPLYETLRGVCSLDAETSSRTHFYAAFTLSTQWKTLEASAICRYDLAEIQAVFAG PYMEYQDGSRRWGRYEGGVPEPRPGSCITDSLRSQGYNSSQDLPSLVLDFVKLHPLMARPVVPTRGRPLL LKRNIRYTHLTGTPVTTPAGPTYDLLFLGTADGWIHKAVVLGSGMHIIEETQVFRESQSVENLVISLLQH SLYVGAPSGVIQLPLSSCSRYRSCYDCILARDPYCGWDPGTHACAAATTIANRSQGSRTALIQDIERGNR GCESSRDTGPPPPLKTRSVLRGDDVLLPCDQPSNLARALWLLNGSMGLSDGQGGYRVGVDGLLVTDAQPE HSGNYGCYAEENGLRTLLASYSLTVRPATPAPAPKAPATPGAQLAPDVRLLYVLAIAALGGLCLILASSL LYVACLREGRRGRRRKYSLGRASRAGGSAVQLQTVSGQCPGEEDEGDDEGAGGLEGSCLQIIPGEGAPAP PPPPPPPPPAELTNGLVALPSRLRRMNGNSYVLLRQSNNGVPAGPCSFAEELSRILEKRKHTQLVEQLDE SSV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017893 |
| ORF Size | 2529 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017893.3 |
| RefSeq Size | 4400 bp |
| RefSeq ORF | 2532 bp |
| Locus ID | 57715 |
| UniProt ID | Q9NTN9 |
| Protein Families | Transmembrane |
| Protein Pathways | Axon guidance |
| Gene Summary | Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210343 | SEMA4G (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (SEMA4G), transcript variant 1 |
CNY 3,990.00 |
|
| RC210343L3 | Lenti-ORF clone of SEMA4G (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (SEMA4G), transcript variant 1 |
CNY 5,890.00 |
|
| RC210343L4 | Lenti-ORF clone of SEMA4G (mGFP-tagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (SEMA4G), transcript variant 1 |
CNY 5,890.00 |
|
| SC113874 | SEMA4G (untagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (SEMA4G), transcript variant 1 |
CNY 1,200.00 |
|
| SC318328 | SEMA4G (untagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (SEMA4G), transcript variant 1 |
CNY 7,220.00 |
