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TFII I (GTF2I) (NM_001518) Human Tagged ORF Clone
CAT＃: RG209126
- TrueORF®
GTF2I (tGFP-tagged) - Human general transcription factor IIi (GTF2I), transcript variant 4
ORF Plasmid: DDK
"NM_001518" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BAP135; BTKAP1; DIWS; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG209126 representing NM_001518
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAAGTTGCAATGTCCACCCTCCCCGTTGAAGATGAGGAGTCCTCGGAGAGCAGGATGGTGGTGA CATTCCTCATGTCAGCTCTCGAGTCCATGTGTAAAGAACTGGCCAAGTCCAAAGCCGAAGTGGCCTGCAT TGCAGTGTATGAAACAGACGTGTTTGTCGTCGGAACTGAAAGAGGACGTGCTTTTGTCAATACCAGAAAG GATTTTCAAAAAGATTTTGTAAAATATTGTGTTGAAGAAGAAGAAAAAGCTGCAGAGATGCATAAAATGA AATCTACAACCCAGGCAAATCGGATGAGTGTAGATGCTGTAGAAATTGAAACACTCAGAAAAACAGTTGA GGACTATTTCTGCTTTTGCTATGGGAAAGCTTTAGGCAAATCCACAGTGGTACCTGTACCATATGAGAAG ATGCTGCGAGACCAGTCGGCTGTGGTAGTGCAGGGGCTTCCGGAAGGTGTTGCCTTTAAACACCCCGAGA ACTATGATCTTGCAACCCTGAAATGGATTTTGGAGAACAAAGCAGGGATTTCATTCATCATTAAGAGACC TTTTTTAGAGCCAAAGAAGCATGTAGGTGGTCGTGTGATGGTAACAGATGCTGACAGGTCAATACTATCT CCAGGTGGAAGTTGTGGCCCCATCAAAGTGAAAACTGAACCCACAGAAGATTCTGGCATTTCCCTGGAAA TGGCAGCTGTGACAGTAAAGGAAGAATCAGAAGATCCTGATTATTATCAATATAACATTCAAGGAAGCCA CCATTCTTCAGAGGGCAATGAAGGCACAGAAATGGAAGTACCAGCAGAAGATGATGATTATTCTCCACCG TCTAAGAGACCAAAGGCCAATGAGCTACCGCAGCCACCAGTCCCGGAACCCGCCAATGCTGGGAAGCGGA AAGTGAGGGAGTTCAACTTCGAGAAATGGAATGCTCGCATCACTGATCTACGTAAACAAGTTGAAGAATT GTTTGAAAGGAAATATGCTCAAGCCATAAAAGCCAAAGGTCCGGTGACGATCCCGTACCCTCTTTTCCAG TCTCATGTTGAAGATCTTTATGTAGAAGGACTTCCTGAAGGAATTCCTTTTAGAAGGCCATCTACTTACG GAATTCCTCGCCTGGAGAGGATATTACTTGCAAAGGAAAGGATTCGTTTTGTGATTAAGAAACATGAGCT TCTGAATTCAACACGTGAAGATTTACAGCTTGATAAGCCAGCTTCAGGAGTAAAGGAAGAATGGTATGCC AGAATCACTAAATTAAGAAAGATGGTGGATCAGCTTTTCTGCAAAAAATTTGCGGAAGCCTTGGGGAGCA CTGAAGCCAAGGCTGTACCGTACCAAAAATTTGAGGCACACCCGAATGATCTGTACGTGGAAGGACTGCC AGAAAACATTCCTTTCCGAAGTCCCTCATGGTATGGAATCCCAAGGCTGGAAAAAATCATTCAAGTGGGC AATCGAATTAAATTTGTTATTAAAAGACCAGAACTTCTGACTCACAGTACCACTGAAGTTACTCAGCCAA GAACGAATACACCAGTCAAAGAAGATTGGAATGTCAGAATTACCAAGCTACGGAAGCAAGTGGAAGAGAT TTTTAATTTGAAATTTGCTCAAGCTCTTGGACTCACCGAGGCAGTAAAAGTACCATATCCTGTGTTTGAA TCAAACCCGGAGTTCTTGTATGTGGAAGGCTTGCCAGAGGGGATTCCCTTCCGAAGCCCTACCTGGTTTG GAATTCCACGACTTGAAAGGATCGTCCGCGGGAGTAATAAAATCAAGTTCGTTGTTAAAAAACCTGAACT AGTTATTTCCTACTTGCCTCCTGGGATGGCTAGTAAAATAAACACTAAAGCTTTGCAGTCCCCCAAAAGA CCACGAAGTCCTGGGAGTAATTCAAAGGTTCCTGAAATTGAGGTCACCGTGGAAGGCCCTAATAACAACA ATCCTCAAACCTCAGCTGTTCGAACCCCGACCCAGACTAACGGTTCTAACGTTCCCTTCAAGCCACGAGG GAGAGAGTTTTCCTTTGAGGCCTGGAATGCCAAAATCACGGACCTAAAACAGAAAGTTGAAAATCTCTTC AATGAGAAATGTGGGGAAGCTCTTGGCCTTAAACAAGCTGTGAAGGTGCCGTTCGCGTTATTTGAGTCTT TCCCGGAAGACTTTTATGTGGAAGGCTTACCTGAGGGTGTGCCATTCCGAAGACCATCGACTTTTGGCAT TCCGAGGCTGGAGAAGATACTCAGAAACAAAGCCAAAATTAAGTTCATCATTAAAAAGCCCGAAATGTTT GAGACGGCGATTAAGGAGAGCACCTCCTCTAAGAGCCCTCCCAGAAAAATAAATTCATCACCCAATGTTA ATACTACTGCATCAGGTGTTGAAGACCTTAACATCATTCAGGTGACAATTCCAGATGATGATAATGAAAG ACTCTCGAAAGTTGAAAAAGCTAGACAGCTAAGAGAACAAGTGAATGACCTCTTTAGTCGGAAATTTGGT GAAGCTATTGGTATGGGTTTTCCTGTGAAAGTTCCCTACAGGAAAATCACAATTAACCCTGGCTGTGTGG TGGTTGATGGCATGCCCCCGGGGGTGTCCTTCAAAGCCCCCAGCTACCTGGAAATCAGCTCCATGAGAAG GATCTTAGACTCTGCCGAGTTTATCAAATTCACGGTCATTAGACCATTTCCAGGACTTGTGATTAATAAC CAGCTGGTTGATCAGAGTGAGTCAGAAGGCCCCGTGATACAAGAATCAGCTGAACCAAGCCAGTTGGAAG TTCCAGCCACAGAAGAAATAAAAGAGACTGATGGAAGCTCTCAGATCAAGCAAGAACCAGACCCCACGTG G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209126 representing NM_001518
Red=Cloning site Green=Tags(s) MAQVAMSTLPVEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRK DFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEK MLRDQSAVVVQGLPEGVAFKHPENYDLATLKWILENKAGISFIIKRPFLEPKKHVGGRVMVTDADRSILS PGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGSHHSSEGNEGTEMEVPAEDDDYSPP SKRPKANELPQPPVPEPANAGKRKVREFNFEKWNARITDLRKQVEELFERKYAQAIKAKGPVTIPYPLFQ SHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKKHELLNSTREDLQLDKPASGVKEEWYA RITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVG NRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLRKQVEEIFNLKFAQALGLTEAVKVPYPVFE SNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSNKIKFVVKKPELVISYLPPGMASKINTKALQSPKR PRSPGSNSKVPEIEVTVEGPNNNNPQTSAVRTPTQTNGSNVPFKPRGREFSFEAWNAKITDLKQKVENLF NEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMF ETAIKESTSSKSPPRKINSSPNVNTTASGVEDLNIIQVTIPDDDNERLSKVEKARQLREQVNDLFSRKFG EAIGMGFPVKVPYRKITINPGCVVVDGMPPGVSFKAPSYLEISSMRRILDSAEFIKFTVIRPFPGLVINN QLVDQSESEGPVIQESAEPSQLEVPATEEIKETDGSSQIKQEPDPTW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001518 |
| ORF Size | 2871 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001518.3 |
| RefSeq Size | 4422 bp |
| RefSeq ORF | 2874 bp |
| Locus ID | 2969 |
| UniProt ID | P78347 |
| Domains | GTF2I |
| Protein Families | Transcription Factors |
| Protein Pathways | Basal transcription factors |
| Gene Summary | This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209126 | GTF2I (Myc-DDK-tagged)-Human general transcription factor IIi (GTF2I), transcript variant 4 |
CNY 3,990.00 |
|
| RC209126L3 | Lenti-ORF clone of GTF2I (Myc-DDK-tagged)-Human general transcription factor IIi (GTF2I), transcript variant 4 |
CNY 5,890.00 |
|
| RC209126L4 | Lenti-ORF clone of GTF2I (mGFP-tagged)-Human general transcription factor IIi (GTF2I), transcript variant 4 |
CNY 5,890.00 |
|
| SC127950 | GTF2I (untagged)-Human general transcription factor IIi (GTF2I), transcript variant 4 |
CNY 7,016.00 |
