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TKTL1 (NM_012253) Human Tagged ORF Clone
CAT＃: RG205218
- TrueORF®
TKTL1 (tGFP-tagged) - Human transketolase-like 1 (TKTL1), transcript variant 1
ORF Plasmid: DDK
"NM_012253" in other vectors (7)
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CNY 8,256.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TKR; TKT2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205218 representing NM_012253
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATGCTGAGGCGAGGGCTGAGTTCCCGGAGGAGGCCAGACCTGACAGGGGCACCTTGCAGGTGT TTCAAGATATGGCCAGCCGCTTGCGAATCCATTCCATCAGGGCCACATGCTCCACGAGCTCCGGCCACCC TACATCATGTAGCAGTTCTTCTGAGATCATGTCTGTGCTGTTCTTCTACATCATGAGGTACAAGCAGTCA GATCCAGAGAATCCGGACAACGACCGATTTGTCCTCGCAAAGAGACTGTCGTTTGTGGATGTGGCAACAG GATGGCTCGGACAAGGACTGGGAGTTGCATGTGGAATGGCATATACTGGCAAGTACTTCGACAGGGCCAG CTACCGGGTGTTCTGCCTCATGAGTGATGGCGAGTCCTCAGAAGGCTCTGTCTGGGAGGCAATGGCCTTT GCTTCCTACTACAGTCTGGACAATCTTGTGGCAACCTTTGATGTGAACCGCCTGGGACACAGTGGTGCAT TGCCCGCCGAGCACTGCATAAACATCTATCAGAGGCGCTGCGAAGCCTTTGGGTGGAACACTTATGTGGT GGACGGCCGGGACGTGGAGGCACTGTGCCAGGTATTCTGGCAGGCTTCTCAGGTGAAGCACAAGCCCACT GCTGTGGTGGCCAAGACCTTCAAGGGCCGGGGCACCCCAAGTATTGAGGATGCAGAAAGTTGGCATGCAA AGCCAATGCCGAGAGAAAGAGCAGATGCCATTATCAAATTAATTGAGAGCCAGATACAGACCAGCAGGAA TCTTGACCCACAGCCCCCCATTGAGGACTCACCTGAAGTCAACATCACAGATGTAAGGATGACCTCTCCA CCTGATTACAGAGTTGGTGACAAGATAGCTACTCGGAAAGCATGCGGTCTGGCTCTGGCTAAGCTGGGCT ACGCGAACAACAGAGTCGTTGTGCTGGATGGTGACACCAGGTACTCTACTTTCTCTGAGATATTCAACAA GGAGTACCCTGAGCGCTTCATCGAGTGCTTTATGGCTGAACAAAACATGGTGAGCGTGGCTCTGGGCTGT GCCTCCCGTGGACGGACCATTGCTTTTGCTAGCACCTTTGCTGCCTTTCTGACTCGAGCATTTGATCACA TCCGGATAGGAGGCCTCGCTGAGAGCAACATCAACATTATTGGTTCCCACTGTGGGGTATCTGTTGGTGA CGATGGTGCTTCCCAGATGGCCCTGGAGGATATAGCCATGTTCCGAACCATTCCCAAGTGCACGATCTTC TACCCAACTGATGCCGTCTCCACGGAGCATGCTGTTGCTCTGGCAGCCAATGCCAAGGGGATGTGCTTCA TTCGGACCACCCGACCAGAAACTATGGTTATTTACACCCCACAAGAACGCTTTGAGATCGGACAGGCCAA GGTCCTCCGCCACTGTGTCAGTGACAAGGTCACAGTTATTGGAGCTGGAATTACTGTGTATGAAGCCTTA GCAGCTGCTGATGAGCTTTCGAAACAAGATATTTTTATCCGTGTCATCGACCTGTTTACCATTAAACCTC TGGATGTCGCCACCATCGTCTCCAGTGCAAAAGCCACAGAGGGCCGGATCATTACAGTGGAGGATCACTA CCCGCAAGGTGGCATCGGGGAAGCTGTCTGCGCAGCCGTCTCCATGGATCCTGACATTCAGGTTCATTCG CTGGCAGTGTCGGGAGTGCCCCAGAGTGGGAAGTCCGAGGAATTGCTGGATATGTATGGAATTAGTGCCA GACATATCATAGTGGCCGTGAAATGCATGTTGCTGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205218 representing NM_012253
Red=Cloning site Green=Tags(s) MADAEARAEFPEEARPDRGTLQVFQDMASRLRIHSIRATCSTSSGHPTSCSSSSEIMSVLFFYIMRYKQS DPENPDNDRFVLAKRLSFVDVATGWLGQGLGVACGMAYTGKYFDRASYRVFCLMSDGESSEGSVWEAMAF ASYYSLDNLVATFDVNRLGHSGALPAEHCINIYQRRCEAFGWNTYVVDGRDVEALCQVFWQASQVKHKPT AVVAKTFKGRGTPSIEDAESWHAKPMPRERADAIIKLIESQIQTSRNLDPQPPIEDSPEVNITDVRMTSP PDYRVGDKIATRKACGLALAKLGYANNRVVVLDGDTRYSTFSEIFNKEYPERFIECFMAEQNMVSVALGC ASRGRTIAFASTFAAFLTRAFDHIRIGGLAESNINIIGSHCGVSVGDDGASQMALEDIAMFRTIPKCTIF YPTDAVSTEHAVALAANAKGMCFIRTTRPETMVIYTPQERFEIGQAKVLRHCVSDKVTVIGAGITVYEAL AAADELSKQDIFIRVIDLFTIKPLDVATIVSSAKATEGRIITVEDHYPQGGIGEAVCAAVSMDPDIQVHS LAVSGVPQSGKSEELLDMYGISARHIIVAVKCMLLN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012253 |
| ORF Size | 1788 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012253.2, NP_036385.2 |
| RefSeq Size | 2530 bp |
| RefSeq ORF | 1791 bp |
| Locus ID | 8277 |
| UniProt ID | P51854 |
| Domains | transketolase, transket_pyr, transketolase_C |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Pentose phosphate pathway |
| Gene Summary | The protein encoded by this gene is a transketolase that acts as a homodimer and catalyzes the conversion of sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate. This reaction links the pentose phosphate pathway with the glycolytic pathway. Variations in this gene may be the cause of Wernicke-Korsakoff syndrome. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Transketolase regulates the metabolic switch to control breast cancer cell metastasis via the alpha-ketoglutarate signaling pathway
,Tseng, CW;Kuo, WH;Chan, SH;Chan, HL;Chang, KJ;Wang, LH;,
Cancer Res.
,PubMed ID 29599405
[TKTL1]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205218 | TKTL1 (Myc-DDK-tagged)-Human transketolase-like 1 (TKTL1), transcript variant 1 |
CNY 6,656.00 |
|
| RC205218L1 | Lenti ORF clone of Human transketolase-like 1 (TKTL1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205218L2 | Lenti ORF clone of Human transketolase-like 1 (TKTL1), transcript variant 1, mGFP tagged |
CNY 9,056.00 |
|
| RC205218L3 | Lenti ORF clone of Human transketolase-like 1 (TKTL1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205218L4 | Lenti ORF clone of Human transketolase-like 1 (TKTL1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC115462 | TKTL1 (untagged)-Human transketolase-like 1 (TKTL1), transcript variant 1 |
CNY 9,712.00 |
|
| SC318026 | TKTL1 (untagged)-Human transketolase-like 1 (TKTL1), transcript variant 1 |
CNY 6,664.00 |
