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RAG2 (NM_000536) Human Tagged ORF Clone
CAT＃: RG205065
- TrueORF®
RAG2 (tGFP-tagged) - Human recombination activating gene 2 (RAG2), transcript variant 1
ORF Plasmid: DDK
"NM_000536" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RAG-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205065 representing NM_000536
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGCAGATGGTAACAGTCAGTAATAACATAGCCTTAATTCAGCCAGGCTTCTCACTGATGAATT TTGATGGACAAGTTTTCTTCTTTGGACAAAAAGGCTGGCCCAAAAGATCCTGCCCCACTGGAGTTTTCCA TCTGGATGTAAAGCATAACCATGTCAAACTGAAGCCTACAATTTTCTCTAAGGATTCCTGCTACCTCCCT CCTCTTCGCTACCCAGCCACTTGCACATTCAAAGGCAGCTTGGAGTCTGAAAAGCATCAATACATCATCC ATGGAGGGAAAACACCAAACAATGAGGTTTCAGATAAGATTTATGTCATGTCTATTGTTTGCAAGAACAA CAAAAAGGTTACTTTTCGCTGCACAGAGAAAGACTTGGTAGGAGATGTTCCTGAAGCCAGATATGGTCAT TCCATTAATGTGGTGTACAGCCGAGGGAAAAGTATGGGTGCTCTCTTTGGAGGACGCTCATACATGCCTT CTACCCACAGAACCACAGAAAAATGGAATAGTGTAGCTGACTGCCTGCCCTGTGTTTTCCTGGTGGATTT TGAATTTGGGTGTGCTACATCATACATTCTTCCAGAACTTCAGGATGGGCTATCTTTTCATGTCTCTATT GCCAAAAATGACACCATCTATATTTTAGGAGGACATTCACTTGCCAATAATATCCGGCCTGCCAACCTGT ACAGAATAAGGGTTGATCTTCCCCTGGGTAGCCCAGCTGTGAATTGCACAGTCTTGCCAGGAGGAATCTC TGTCTCCAGTGCAATCCTGACTCAAACTAACAATGATGAATTTGTTATTGTTGGTGGCTATCAGCTTGAA AATCAAAAAAGAATGATCTGCAACATCATCTCTTTAGAGGACAACAAGATAGAAATTCGTGAGATGGAGA CCCCAGATTGGACCCCAGACATTAAGCACAGCAAGATATGGTTTGGAAGCAACACGGGAAATGGAACTGT TTTTCTTGGCATACCAGGAGACAATAAACAAGTTGTTTCAGAAGGATTCTATTTCTATATGTTGAAATGT GCTGAAGATGATACTAATGAAGAGCAGACAACATTCACAAACAGTCAAACATCAACAGAAGATCCAGGGG ATTCCACTCCCTTTGAAGACTCTGAAGAATTTTGTTTCAGTGCAGAAGCAAATAGTTTTGATGGTGATGA TGAATTTGACACCTATAATGAAGATGATGAAGAAGATGAGTCTGAGACAGGCTACTGGATTACATGCTGC CCTACTTGTGATGTGGATATCAACACTTGGGTACCATTCTATTCAACTGAGCTCAACAAACCCGCCATGA TCTACTGCTCTCATGGGGATGGGCACTGGGTCCATGCTCAGTGCATGGATCTGGCAGAACGCACACTCAT CCATCTGTCAGCAGGAAGCAACAAGTATTACTGCAATGAGCATGTGGAGATAGCAAGAGCTCTACACACT CCCCAAAGAGTCCTACCCTTAAAAAAGCCTCCAATGAAATCCCTCCGTAAAAAAGGTTCTGGAAAAATCT TGACTCCTGCCAAGAAATCCTTTCTTAGAAGGTTGTTTGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205065 representing NM_000536
Red=Cloning site Green=Tags(s) MSLQMVTVSNNIALIQPGFSLMNFDGQVFFFGQKGWPKRSCPTGVFHLDVKHNHVKLKPTIFSKDSCYLP PLRYPATCTFKGSLESEKHQYIIHGGKTPNNEVSDKIYVMSIVCKNNKKVTFRCTEKDLVGDVPEARYGH SINVVYSRGKSMGALFGGRSYMPSTHRTTEKWNSVADCLPCVFLVDFEFGCATSYILPELQDGLSFHVSI AKNDTIYILGGHSLANNIRPANLYRIRVDLPLGSPAVNCTVLPGGISVSSAILTQTNNDEFVIVGGYQLE NQKRMICNIISLEDNKIEIREMETPDWTPDIKHSKIWFGSNTGNGTVFLGIPGDNKQVVSEGFYFYMLKC AEDDTNEEQTTFTNSQTSTEDPGDSTPFEDSEEFCFSAEANSFDGDDEFDTYNEDDEEDESETGYWITCC PTCDVDINTWVPFYSTELNKPAMIYCSHGDGHWVHAQCMDLAERTLIHLSAGSNKYYCNEHVEIARALHT PQRVLPLKKPPMKSLRKKGSGKILTPAKKSFLRRLFD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000536 |
| ORF Size | 1581 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000536.1, NP_000527.1 |
| RefSeq Size | 2414 bp |
| RefSeq ORF | 1584 bp |
| Locus ID | 5897 |
| UniProt ID | P55895 |
| Protein Families | Druggable Genome |
| Protein Pathways | Primary immunodeficiency |
| Gene Summary | This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205065 | RAG2 (Myc-DDK-tagged)-Human recombination activating gene 2 (RAG2), transcript variant 1 |
CNY 3,928.00 |
|
| RC205065L1 | Lenti ORF clone of Human recombination activating gene 2 (RAG2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205065L2 | Lenti ORF clone of Human recombination activating gene 2 (RAG2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC205065L3 | Lenti ORF clone of Human recombination activating gene 2 (RAG2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205065L4 | Lenti ORF clone of Human recombination activating gene 2 (RAG2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC122564 | RAG2 (untagged)-Human recombination activating gene 2 (RAG2), transcript variant 1 |
CNY 7,220.00 |
