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ACTC1 (NM_005159) Human Tagged ORF Clone
CAT＃: RG203655
- TrueORF®
ACTC1 (tGFP-tagged) - Human actin, alpha, cardiac muscle 1 (ACTC1)
ORF Plasmid: DDK
"NM_005159" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ACTC; ASD5; CMD1R; CMH11; LVNC4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203655 representing NM_005159
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGACGACGAGGAGACCACCGCCCTGGTGTGCGACAACGGCTCTGGGCTGGTGAAGGCCGGCTTTG CGGGCGATGACGCGCCCCGCGCTGTCTTCCCGTCCATCGTGGGCCGCCCGCGGCACCAGGGAGTTATGGT GGGTATGGGTCAGAAGGACTCCTACGTAGGTGATGAAGCCCAGAGCAAGAGAGGCATCCTGACCCTGAAG TATCCCATCGAGCATGGTATCATCACCAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACA ATGAGCTCCGTGTGGCTCCCGAGGAGCACCCCACCCTGCTCACAGAGGCCCCGCTGAACCCCAAGGCCAA CCGGGAGAAGATGACTCAGATCATGTTTGAGACCTTCAATGTCCCTGCCATGTACGTGGCCATCCAGGCA GTGCTATCCCTGTATGCTTCTGGCCGTACCACAGGCATTGTTCTGGACTCTGGGGATGGTGTAACTCACA ATGTCCCCATCTATGAGGGCTACGCTTTGCCCCATGCCATCATGCGTCTGGATCTGGCTGGTCGGGACCT CACTGACTACCTCATGAAGATCCTCACTGAGCGTGGCTACTCCTTTGTCACCACTGCTGAACGTGAAATT GTCCGTGACATTAAAGAGAAGCTGTGCTATGTCGCCCTGGATTTTGAGAATGAGATGGCCACAGCTGCCT CTTCCTCCTCCCTGGAGAAGAGCTATGAACTGCCTGATGGCCAAGTCATCACTATTGGCAATGAGCGCTT CCGCTGTCCTGAGACACTCTTCCAGCCCTCCTTCATTGGTATGGAATCTGCTGGCATCCATGAAACAACT TACAATAGCATCATGAAGTGTGACATTGATATCCGCAAGGACCTGTATGCCAACAATGTCTTATCTGGAG GCACCACTATGTACCCTGGTATTGCTGATCGTATGCAGAAGGAAATCACTGCTCTGGCTCCTAGCACCAT GAAGATTAAGATTATTGCTCCCCCTGAGCGTAAATACTCTGTCTGGATTGGGGGCTCCATCCTGGCCTCT CTGTCCACCTTCCAGCAAATGTGGATTAGCAAGCAAGAGTACGATGAGGCAGGCCCATCCATTGTCCACC GCAAATGCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203655 representing NM_005159
Red=Cloning site Green=Tags(s) MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLK YPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQA VLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREI VRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETT YNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAS LSTFQQMWISKQEYDEAGPSIVHRKCF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005159 |
| ORF Size | 1131 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005159.5 |
| RefSeq Size | 3693 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 70 |
| UniProt ID | P68032 |
| Domains | ACTIN |
| Protein Pathways | Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM) |
| Gene Summary | Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203655 | ACTC1 (Myc-DDK-tagged)-Human actin, alpha, cardiac muscle 1 (ACTC1) |
CNY 3,656.00 |
|
| RC203655L3 | Lenti ORF clone of Human actin, alpha, cardiac muscle 1 (ACTC1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203655L4 | Lenti ORF clone of Human actin, alpha, cardiac muscle 1 (ACTC1), mGFP tagged |
CNY 5,890.00 |
|
| SC319325 | ACTC1 (untagged)-Human actin, alpha, cardiac muscle 1 (ACTC1) |
CNY 2,950.00 |
