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BBOX1 (NM_003986) Human Tagged ORF Clone
CAT＃: RG203156
- TrueORF®
BBOX1 (tGFP-tagged) - Human butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (BBOX1)
ORF Plasmid: DDK
"NM_003986" in other vectors (4)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BBH; BBOX; G-BBH; gamma-BBH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203156 representing NM_003986
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGTACCATCCAAAAGGCAGAAGCACTTGACGGGGCTCATTTGATGCAGATCCTCTGGTATGATG AGGAAGAGTCTCTCTACCCAGCTGTATGGTTGAGAGACAACTGTCCGTGCTCTGATTGCTACCTGGATTC TGCAAAAGCACGGAAACTTCTAGTGGAAGCTCTTGATGTGAACATTGGAATTAAAGGCTTGATATTTGAC AGAAAAAAGGTGTACATCACATGGCCCGATGAGCATTACAGTGAATTCCAGGCTGATTGGCTGAAGAAAA GATGCTTTTCCAAGCAGGCCAGAGCAAAGCTCCAAAGAGAATTGTTTTTTCCAGAATGCCAATACTGGGG CTCAGAGCTCCAGCTACCCACTTTGGATTTTGAAGATGTTTTAAGATATGATGAACATGCATACAAGTGG CTCTCCACCCTCAAGAAAGTAGGCATAGTAAGACTCACCGGAGCATCTGACAAACCAGGAGAAGTTTCAA AACTTGGGAAAAGGATGGGTTTCCTCTATCTCACATTTTATGGACATACTTGGCAAGTGCAAGACAAAAT CGATGCAAACAATGTGGCTTACACAACTGGGAAGCTAAGCTTTCACACTGATTATCCAGCCCTCCATCAT CCACCTGGGGTTCAGCTTCTTCACTGCATAAAGCAAACAGTCACAGGGGGTGATTCAGAAATTGTAGATG GGTTTAATGTGTGCCAAAAACTAAAGAAAAATAATCCTCAGGCATTCCAGATTTTGTCCTCTACCTTTGT GGACTTTACAGACATTGGAGTGGATTACTGTGATTTTTCTGTACAATCAAAACATAAAATTATAGAGTTA GATGATAAAGGCCAAGTGGTTCGCATCAACTTCAATAACGCAACTAGGGACACAATATTTGATGTACCTG TTGAAAGAGTTCAGCCTTTTTATGCTGCTCTGAAGGAGTTTGTTGACCTCATGAACAGCAAAGAATCCAA GTTTACCTTCAAGATGAATCCAGGTGATGTGATTACTTTTGATAACTGGCGCTTACTTCATGGCCGACGT AGCTATGAAGCAGGAACTGAGATATCCCGCCATCTAGAAGGAGCTTATGCTGACTGGGATGTGGTCATGT CAAGGCTTCGTATCTTAAGGCAGAGGGTGGAGAATGGAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203156 representing NM_003986
Red=Cloning site Green=Tags(s) MACTIQKAEALDGAHLMQILWYDEEESLYPAVWLRDNCPCSDCYLDSAKARKLLVEALDVNIGIKGLIFD RKKVYITWPDEHYSEFQADWLKKRCFSKQARAKLQRELFFPECQYWGSELQLPTLDFEDVLRYDEHAYKW LSTLKKVGIVRLTGASDKPGEVSKLGKRMGFLYLTFYGHTWQVQDKIDANNVAYTTGKLSFHTDYPALHH PPGVQLLHCIKQTVTGGDSEIVDGFNVCQKLKKNNPQAFQILSSTFVDFTDIGVDYCDFSVQSKHKIIEL DDKGQVVRINFNNATRDTIFDVPVERVQPFYAALKEFVDLMNSKESKFTFKMNPGDVITFDNWRLLHGRR SYEAGTEISRHLEGAYADWDVVMSRLRILRQRVENGN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003986 |
| ORF Size | 1161 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003986.1, NP_003977.1 |
| RefSeq Size | 1901 bp |
| RefSeq ORF | 1164 bp |
| Locus ID | 8424 |
| UniProt ID | O75936 |
| Protein Pathways | Lysine degradation |
| Gene Summary | This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203156 | BBOX1 (Myc-DDK-tagged)-Human butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (BBOX1) |
CNY 3,656.00 |
|
| RC203156L3 | Lenti ORF clone of Human butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (BBOX1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203156L4 | Lenti ORF clone of Human butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (BBOX1), mGFP tagged |
CNY 5,890.00 |
|
| SC319674 | BBOX1 (untagged)-Human butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (BBOX1) |
CNY 3,656.00 |
