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ECSIT (NM_016581) Human Tagged ORF Clone
CAT＃: RG201343
- TrueORF®
ECSIT (tGFP-tagged) - Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid: DDK
"NM_016581" in other vectors (6)
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CNY 7,456.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SITPEC |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201343 representing NM_016581
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTGGGTCCAGGCCACCCTACTGGCCCGAGGCCTCTGTAGGGCCTGGGGAGGCACCTGCGGGGCCG CCCTCACAGGAACCTCCATCTCTCAGGTCCCTCGCCGGCTCCCTCGGGGCCTCCACTGCAGCGCAGCTGC CCATAGCTCTGAACAGTCCCTGGTTCCCAGCCCACCGGAACCCCGGCAGAGGCCCACCAAGGCTCTGGTG CCCTTTGAGGACCTGTTTGGGCAGGCGCCTGGTGGGGAACGGGACAAGGCGAGCTTCCTGCAGACGGTGC AGAAATTTGCGGAGCACAGCGTGCGTAAGCGGGGCCACATTGACTTCATCTACCTGGCCCTGCGCAAGAT GCGGGAGTATGGTGTCGAGCGGGACCTGGCTGTGTACAACCAGCTGCTCAACATCTTCCCCAAGGAGGTC TTCCGGCCTCGCAACATCATCCAGCGCATCTTCGTCCACTACCCTCGGCAGCAGGAGTGTGGGATTGCTG TCCTGGAGCAGATGGAGAACCACGGTGTGATGCCCAACAAGGAGACGGAGTTCCTGCTGATTCAGATCTT TGGACGCAAAAGCTACCCCATGCTCAAGTTGGTGCGCCTGAAGCTGTGGTTCCCTCGATTCATGAACGTC AACCCCTTCCCAGTGCCCCGGGACCTGCCCCAGGACCCTGTGGAGCTGGCCATGTTTGGCCTGCGGCACA TGGAGCCTGACCTTAGTGCCAGGGTCACCATCTACCAGGTTCCTTTGCCCAAAGACTCAACAGGTGCAGC AGATCCCCCCCAGCCCCACATCGTAGGAATCCAGAGTCCCGATCAGCAGGCCGCCCTGGCCCGCCACAAT CCAGCCCGGCCTGTCTTTGTTGAGGGCCCCTTCTCCCTGTGGCTCCGCAACAAGTGTGTGTATTACCACA TCCTCAGAGCTGACTTGCTGCCCCCGGAGGAGAGGGAAGTGGAAGAGACGCCGGAGGAGTGGAACCTCTA CTACCCGATGCAGCTGGACCTGGAGTATGTGAGGAGTGGCTGGGACAACTACGAGTTTGACATCAATGAA GTGGAGGAAGGCCCTGTCTTCGCCATGTGCATGGCGGGTGCTCATGACCAGGCGACGATGGCTAAGTGGA TCCAGGGCCTGCAGGAGACCAACCCAACCCTGGCCCAGATCCCCGTGGTCTTCCGCCTCGCCGGGTCCAC CCGGGAGCTCCAGACATCCTCTGCAGGGCTGGAGGAGCCGCCCCTGCCCGAGGACCACCAGGAAGAAGAC GACAACCTGCAGCGACAGCAGCAGGGCCAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201343 representing NM_016581
Red=Cloning site Green=Tags(s) MSWVQATLLARGLCRAWGGTCGAALTGTSISQVPRRLPRGLHCSAAAHSSEQSLVPSPPEPRQRPTKALV PFEDLFGQAPGGERDKASFLQTVQKFAEHSVRKRGHIDFIYLALRKMREYGVERDLAVYNQLLNIFPKEV FRPRNIIQRIFVHYPRQQECGIAVLEQMENHGVMPNKETEFLLIQIFGRKSYPMLKLVRLKLWFPRFMNV NPFPVPRDLPQDPVELAMFGLRHMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHN PARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPMQLDLEYVRSGWDNYEFDINE VEEGPVFAMCMAGAHDQATMAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSSAGLEEPPLPEDHQEED DNLQRQQQGQS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016581 |
| ORF Size | 1293 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016581.5 |
| RefSeq Size | 1710 bp |
| RefSeq ORF | 1296 bp |
| Locus ID | 51295 |
| UniProt ID | Q9BQ95 |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201343 | ECSIT (Myc-DDK-tagged)-Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,856.00 |
|
| RC201343L1 | Lenti-ORF clone of ECSIT (Myc-DDK-tagged)-Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 8,256.00 |
|
| RC201343L2 | Lenti-ORF clone of ECSIT (mGFP-tagged)-Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
| RC201343L3 | Lenti-ORF clone of ECSIT (Myc-DDK-tagged)-Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
| RC201343L4 | Lenti-ORF clone of ECSIT (mGFP-tagged)-Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
| SC122849 | ECSIT (untagged)-Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,488.00 |
