AP3B2 (NM_001278511) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DEE48; EIEE48; NAPTB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239916 representing NM_001278511 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCGCCCCCGCCTACAGCGAAGACAAGGGCGGCTCCGCTGGCCCCGGGGAGCCCGAGTACGGCC ACGACCCCGCGAGCGGCGGCATCTTCTCCTCCGACTACAAGCGGCATGATGACCTGAAGGAGATGCTGGA CACCAACAAGGATTCTCTCAAGCTGGAGGCCATGAAGAGGATTGTGGCGATGATTGCCCGAGGAAAGAAT GCTTCAGACCTGTTTCCCGCGGTGGTGAAGAACGTGGCCTGTAAGAACATAGAGGATCCCAACCAGCTGA TTCGTGCCAGTGCCCTCCGTGTCCTCTCTAGCATCCGTGTGCCCATCATAGTGCCCATCATGATGCTAGC TATCAAGGAAGCCGCCTCGGACATGTCACCCTATGTGCGGAAAACAGCTGCCCACGCCATCCCTAAACTC TACAGTTTGGACTCTGACCAGAAGGATCAGCTGATAGAAGTCATTGAGAAGCTTCTGGCTGACAAGACCA CGCTGGTGGCGGGCAGTGTGGTGATGGCCTTTGAGGAGGTCTGCCCGGAGCGCATCGACCTGATTCACAA AAACTACCGGAAACTCTGTAACCTGCTGATCGACGTGGAGGAGTGGGGCCAGGTGGTCATCATCAGCATG CTCACCCGCTACGCCCGCACGCAGTTCCTGAGCCCCACCCAGAACGAATCCCTACTAGAGGAGAACGCGG AAAAAGCCTTCTACGGCTCAGAGGAGGACGAGGCCAAGGGCGCGGGGTCTGAGGAGACGGCCGCCGCGGC CGCCCCCTCCCGAAAGCCCTATGTCATGGACCCCGACCACCGGCTGCTGCTGCGCAACACCAAACCCCTG CTGCAGAGCCGCAGCGCCGCGGTGGTGATGGCGGTGGCGCAGCTCTACTTCCACCTGGCGCCCAAGGCGG AAGTGGGCGTCATCGCCAAGGCGCTGGTGCGCCTGCTGCGCAGCCACAGTGAGGTGCAGTACGTTGTGCT CCAGAACGTGGCCACCATGTCCATCAAGCGCCGGGGTATGTTTGAGCCCTACCTGAAGAGCTTCTACATC AGGTCCACCGACCCCACCCAGATTAAGATCCTGAAGCTGGAAGTGCTGACCAACCTGGCCAATGAGACCA ACATTCCTACTGTCCTACGGGAATTCCAGACCTATATTCGCAGCATGGACAAGGACTTTGTGGCAGCCAC AATCCAGGCCATTGGACGCTGTGCAACTAACATCGGCCGAGTCCGTGACACCTGCCTCAATGGCCTGGTG CAGCTGCTGTCCAACCGTGATGAGCTTGTGGTTGCAGAGTCAGTGGTCGTCATTAAGAAATTGCTACAGA TGCAGCCAGCACAACATGGAGAGATCATCAAACACTTGGCAAAGCTTACAGACAACATCCAGGTGCCCAT GGCCCGAGCCAGCATCCTGTGGCTCATCGGAGAGTACTGTGAGCATGTCCCCAGGATTGCACCTGATGTC TTAAGAAAAATGGCCAAGTCATTCACAGCAGAGGAGGATATTGTCAAGCTGCAGGTCATCAACCTGGCAG CCAAGCTCTACCTGACCAACTCTAAACAGACCAAGCTGCTGACCCAGTATGTGCTGAGTCTGGCCAAATA TGACCAGAACTATGATATTCGCGACCGGGCGCGCTTCACCCGGCAGCTCATCGTCCCTTCCGAGCAGGGT GGGGCCCTCAGCCGCCATGCCAAGAAGCTCTTCCTGGCACCCAAACCAGCTCCAGTCTTGGAGTCATCCT TCAAAGACCGGGACCACTTCCAGCTGGGCTCACTGTCCCACCTGCTTAATGCCAAGGCCACAGGCTACCA GGAGCTCCCAGACTGGCCGGAGGAAGCCCCAGACCCATCTGTGCGCAACGTGGAGGTACCTGAATGGACC AAGTGCTCAAATCGGGAGAAGAGAAAGGAGAAGGAAAAACCCTTCTACTCGGACTCTGAGGGGGAGTCAG GCCCCACGGAGTCCGCAGACAGTGACCCTGAGTCTGAGAGTGAATCGGACAGTAAGAGCAGCAGTGAGAG CGGCTCTGGGGAGTCCAGCAGTGAGTCCGACAATGAAGACCAGGATGAGGATGAGGAGAAAGGGAGAGGC AGTGAGAGTGAACAGAGTGAGGAGGATGGTAAGAGGAAGACAAAGAAGAAGGTGCCAGAGAGAAAAGGAG AAGCGTCATCCTCTGATGAGGGCAGCGATTCCAGCAGTAGCTCATCAGAGTCCGAGATGACATCGGAGTC CGAGGAGGAGCAGTTAGAACCTGCCTCCTGGAGCAGGAAAACACCTCCCAGCAGCAAAAGTGCTCCTGCA ACCAAGGAGATCTCCCTGCTTGATCTAGAGGATTTCACCCCTCCCAGTGTCCAGCCTGTGTCTCCCCCAG CAATTGTGTCTACCAGTCTGGCTGCTGACCTGGAGGGCCTGACACTCACAGACTCCACCCTGGTACCGTC GCTTCTGAGTCCAGTATCGGGTGTTGGGCGGCAGGAGCTGCTGCACCGGGTAGCTGGCGAGGGGCTGGCT GTGGACTACACCTTCAGCCGCCAACCTTTCTCCGGGGATCCCCACATGGTGTCCGTGCACATCCACTTCT CCAACAGCTCTGATACCCCCATCAAGGGCCTGCATGTGGGCACTCCCAAACTGCCTGCTGGCATCAGCAT CCAAGAATTTCCCGAAATTGAGTCCCTGGCACCTGGAGAATCTGCCACTGCTGTAATGGGCATTAATTTC TGTGACTCAACCCAGGCAGCCAACTTCCAGCTGTGCACCCAGACCCGACAGTTCTACGTCTCCATTCAGC CACCTGTTGGGGAGCTGATGGCCCCTGTGTTCATGAGTGAAAATGAGTTTAAGAAGGAACAGGGAAAGCT GATGGGCATGAATGAGATCACAGAGAAACTCATGCTGCCAGACACCTGTCGGAGTGACCACATTGTGGTG CAGAAAGTGACTGCCACTGCCAACCTGGGTCGTGTTCCTTGTGGGACATCTGATGAGTACAGGTTTGCAG GGAGGACACTGACTGGTGGAAGCCTCGTTCTGCTGACCCTGGATGCCCGGCCAGCTGGAGCTGCCCAGCT GACTGTCAACAGCGAGAAAATGGTGATTGGCACCATGCTGGTAAAGGATGTGATACAGGCTCTGACCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239916 representing NM_001278511 Red=Cloning site Green=Tags(s) MSAAPAYSEDKGGSAGPGEPEYGHDPASGGIFSSDYKRHDDLKEMLDTNKDSLKLEAMKRIVAMIARGKN ASDLFPAVVKNVACKNIEDPNQLIRASALRVLSSIRVPIIVPIMMLAIKEAASDMSPYVRKTAAHAIPKL YSLDSDQKDQLIEVIEKLLADKTTLVAGSVVMAFEEVCPERIDLIHKNYRKLCNLLIDVEEWGQVVIISM LTRYARTQFLSPTQNESLLEENAEKAFYGSEEDEAKGAGSEETAAAAAPSRKPYVMDPDHRLLLRNTKPL LQSRSAAVVMAVAQLYFHLAPKAEVGVIAKALVRLLRSHSEVQYVVLQNVATMSIKRRGMFEPYLKSFYI RSTDPTQIKILKLEVLTNLANETNIPTVLREFQTYIRSMDKDFVAATIQAIGRCATNIGRVRDTCLNGLV QLLSNRDELVVAESVVVIKKLLQMQPAQHGEIIKHLAKLTDNIQVPMARASILWLIGEYCEHVPRIAPDV LRKMAKSFTAEEDIVKLQVINLAAKLYLTNSKQTKLLTQYVLSLAKYDQNYDIRDRARFTRQLIVPSEQG GALSRHAKKLFLAPKPAPVLESSFKDRDHFQLGSLSHLLNAKATGYQELPDWPEEAPDPSVRNVEVPEWT KCSNREKRKEKEKPFYSDSEGESGPTESADSDPESESESDSKSSSESGSGESSSESDNEDQDEDEEKGRG SESEQSEEDGKRKTKKKVPERKGEASSSDEGSDSSSSSSESEMTSESEEEQLEPASWSRKTPPSSKSAPA TKEISLLDLEDFTPPSVQPVSPPAIVSTSLAADLEGLTLTDSTLVPSLLSPVSGVGRQELLHRVAGEGLA VDYTFSRQPFSGDPHMVSVHIHFSNSSDTPIKGLHVGTPKLPAGISIQEFPEIESLAPGESATAVMGINF CDSTQAANFQLCTQTRQFYVSIQPPVGELMAPVFMSENEFKKEQGKLMGMNEITEKLMLPDTCRSDHIVV QKVTATANLGRVPCGTSDEYRFAGRTLTGGSLVLLTLDARPAGAAQLTVNSEKMVIGTMLVKDVIQALTQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278511
ORF Size	3150 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278511.1, NP_001265440.1
RefSeq Size	3675 bp
RefSeq ORF	3153 bp
Locus ID	8120
UniProt ID	Q13367
Protein Pathways	Lysosome
MW	115.8 kDa
Gene Summary	Adaptor protein complex 3 (AP-3 complex) is a heterotrimeric protein complex involved in the formation of clathrin-coated synaptic vesicles. The protein encoded by this gene represents the beta subunit of the neuron-specific AP-3 complex and was first identified as the target antigen in human paraneoplastic neurologic disorders. The encoded subunit binds clathrin and is phosphorylated by a casein kinase-like protein, which mediates synaptic vesicle coat assembly. Defects in this gene are a cause of early-onset epileptic encephalopathy. [provided by RefSeq, Feb 2017]

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