MTR (NM_001291940) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	cblG; HMAG; MS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239695 representing NM_001291940 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCCCAGGTGTTGGATGTCAACATGGATGATGGCATGCTAGATGGTCCAAGTGCAATGACCAGAT TTTGCAACTTAATTGCTTCCGAGCCAGACATCGCAAAGGTACCTTTGTGCATCGACTCCTCCAATTTTGC TGTGATTGAAGCTGGGTTAAAGTGCTGCCAAGGGAAGTGCATTGTCAATAGCATTAGTCTGAAGGAAGGA GAGGACGACTTCTTGGAGAAGGCCAGGAAGATTAAAAAGTATGGAGCTGCTATGGTGGTCATGGCTTTTG ATGAAGAAGGACAGGCAACAGAAACAGACACAAAAATCAGAGTGTGCACCCGGGCCTACCATCTGCTTGT GAAAAAACTGGGCTTTAATCCAAATGACATTATTTTTGACCCTAATATCCTAACCATTGGGACTGGAATG GAGGAACACAACTTGTATGCCATTAATTTTATCCATGCAACAAAAGTCATTAAAGAAACATTACCTGGAG CCAGAATAAGTGGAGGTCTTTCCAACTTGTCCTTCTCCTTCCGAGGAATGGAAGCCATTCGAGAAGCAAT GCATGGGGTTTTCCTTTACCATGCAATCAAGTCTGGCATGGACATGGGGATAGTGAATGCTGGAAACCTC CCTGTGTATGATGATATCCATAAGGAACTTCTGCAGCTCTGTGAAGATCTCATCTGGAATAAAGACCCTG AGGCCACTGAGAAGCTCTTACGTTATGCCCAGACTCAAGGCACAGGAGGGAAGAAAGTCATTCAGACTGA TGAGTGGAGAAATGGCCCTGTCGAAGAACGCCTTGAGTATGCCCTTGTGAAGGGCATTGAAAAACATATT ATTGAGGATACTGAGGAAGCCAGGTTAAACCAAAAAAAATATCCCCGACCTCTCAATATAATTGAAGGAC CCCTGATGAATGGAATGAAAATTGTTGGTGATCTTTTTGGAGCTGGAAAAATGTTTCTACCTCAGGTTAT AAAGTCAGCCCGGGTTATGAAGAAGGCTGTTGGCCACCTTATCCCTTTCATGGAAAAAGAAAGAGAAGAA ACCAGAGTGCTTAACGGCACAGTAGAAGAAGAGGACCCTTACCAGGGCACCATCGTGCTGGCCACTGTTA AAGGCGACGTGCACGACATAGGCAAGAACATAGTTGGAGTAGTCCTTGGCTGCAATAATTTCCGAGTTAT TGATTTAGGAGTCATGACTCCATGTGATAAGATACTGAAAGCTGCTCTTGACCACAAAGCAGATATAATT GGCCTGTCAGGACTCATCACTCCTTCCCTGGATGAAATGATTTTTGTTGCCAAGGAAATGGAGAGATTAG CTATAAGGATTCCATTGTTGATTGGAGGAGCAACCACTTCAAAAACCCACACAGCAGTTAAAATAGCTCC GAGATACAGTGCACCTGTAATCCATGTCCTGGACGCGTCCAAGAGTGTGGTGGTGTGTTCCCAGCTGTTA GATGAAAATCTAAAGGATGAATACTTTGAGGAAATCATGGAAGAATATGAAGATATTAGACAGGACCATT ATGAGTCTCTCAAGGAGAGGAGATACTTACCCTTAAGTCAAGCCAGAAAAAGTGGTTTCCAAATGGATTG GCTGTCTGAACCTCACCCAGTGAAGCCCACGTTTATTGGGACCCAGGTCTTTGAAGACTATGACCTGCAG AAGCTGGTGGACTACATTGACTGGAAGCCTTTCTTTGATGTCTGGCAGCTCCGGGGCAAGTACCCGAATC GAGGCTTTCCCAAGATATTTAACGACAAAACAGTAGGTGGAGAGGCCAGGAAGGTCTACGATGATGCCCA CAATATGCTGAACACACTGATTAGTCAAAAGAAACTCCGGGCCCGGGGTGTGGTTGGGTTCTGGCCAGCA CAGAGTATCCAAGACGACATTCACCTGTACGCAGAGGCTGCTGTGCCCCAGGCTGCAGAGCCCATAGCCA CCTTCTATGGGTTAAGGCAACAGGCTGAGAAGGACTCTGCCAGCACGGAGCCATACTACTGCCTCTCAGA CTTCATCGCTCCCTTGCATTCTGGCATCCGTGACTACCTGGGCCTGTTTGCCGTTGCCTGCTTTGGGGTA GAAGAGCTGAGCAAGGCCTATGAGGATGATGGTGACGACTACAGCAGCATCATGGTCAAGGCGCTGGGGG ACCGGCTGGCAGAGGCCTTTGCAGAAGAGCTCCATGAAAGAGTTCGCCGAGAACTGTGGGCCTACTGTGG CAGTGAGCAGCTGGACGTCGCAGACCTGCGCAGGCTGCGGTACAAGGGCATCCGCCCGGCTCCTGGCTAC CCCAGCCAGCCCGACCACACCGAGAAGCTCACCATGTGGAGACTCGCAGACATCGAGCAGTCTACAGGCA TTAGGTTAACAGAATCATTAGCAATGGCACCTGCTTCAGCAGTCTCAGGCCTCTACTTCTCCAATTTGAA GTCCAAATATTTTGCTGTGGGGAAGATTTCCAAGGATCAGGTTGAGGATTATGCATTGAGGAAGAACATA TCTGTGGCTGAGGTTGAGAAATGGCTTGGACCCATTTTGGGATATGATACAGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239695 representing NM_001291940 Red=Cloning site Green=Tags(s) MGAQVLDVNMDDGMLDGPSAMTRFCNLIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEG EDDFLEKARKIKKYGAAMVVMAFDEEGQATETDTKIRVCTRAYHLLVKKLGFNPNDIIFDPNILTIGTGM EEHNLYAINFIHATKVIKETLPGARISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKSGMDMGIVNAGNL PVYDDIHKELLQLCEDLIWNKDPEATEKLLRYAQTQGTGGKKVIQTDEWRNGPVEERLEYALVKGIEKHI IEDTEEARLNQKKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREE TRVLNGTVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADII GLSGLITPSLDEMIFVAKEMERLAIRIPLLIGGATTSKTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLL DENLKDEYFEEIMEEYEDIRQDHYESLKERRYLPLSQARKSGFQMDWLSEPHPVKPTFIGTQVFEDYDLQ KLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKTVGGEARKVYDDAHNMLNTLISQKKLRARGVVGFWPA QSIQDDIHLYAEAAVPQAAEPIATFYGLRQQAEKDSASTEPYYCLSDFIAPLHSGIRDYLGLFAVACFGV EELSKAYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRLRYKGIRPAPGY PSQPDHTEKLTMWRLADIEQSTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQVEDYALRKNI SVAEVEKWLGPILGYDTD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291940
ORF Size	2574 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291940.2
RefSeq Size	10462 bp
RefSeq ORF	2577 bp
Locus ID	4548
UniProt ID	Q99707
Protein Families	Druggable Genome
Protein Pathways	Cysteine and methionine metabolism, Metabolic pathways, One carbon pool by folate
MW	96.6 kDa
Gene Summary	This gene encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

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