ENTPD3 (NM_001291960) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CD39L3; HB6; NTPDase-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238734 representing NM_001291960 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCACTGTGCTGACCCGCCAACCATGTGAGCAAGCAGGCCTCAAGGCCCTCTACCGAACTCCAACCA TCATTGCCTTGGTGGTCTTGCTTGTGAGTATTGTGGTACTTGTGAGTATCACTGTCATCCAGATCCACAA GCAAGAGGTCCTCCCTCCAGGACTGAAGTATGGTATTGTGCTGGATGCCGGGTCTTCAAGAACCACAGTC TACGTGTATCAATGGCCAGCAGAAAAAGAGAATAATACCGGAGTGGTCAGTCAAACCTTCAAATGTAGTG TGAAAGGCTCTGGAATCTCCAGCTATGGAAATAACCCCCAAGATGTCCCCAGAGCCTTTGAGGAGTGTAT GCAAAAAGTCAAGGGGCAGGTTCCATCCCACCTCCACGGATCCACCCCCATTCACCTGGGAGCCACGGCT GGGATGCGCTTGCTGAGGTTGCAAAATGAAACAGCAGCTAATGAAGTCCTTGAAAGCATCCAAAGCTACT TCAAGTCCCAGCCCTTTGACTTTAGGGGTGCTCAAATCATTTCTGGGCAAGAAGAAGGGGTATATGGATG GATTACAGCCAACTATTTAATGGGAAATTTCCTGGAGAAGAACCTGTGGCACATGTGGGTGCACCCGCAT GGAGTGGAAACCACGGGTGCCCTGGACTTAGGTGGTGCCTCCACCCAAATATCCTTCGTGGCAGGAGAGA AGATGGATCTGAACACCAGCGACATCATGCAGGTGTCCCTGTATGGCTACGTATACACGCTCTACACACA CAGCTTCCAGTGCTATGGCCGGAATGAGGCTGAGAAGAAGTTTCTGGCAATGCTCCTGCAGAATTCTCCT ACCAAAAACCATCTCACCAATCCCTGTTACCCTCGGGATTATAGCATCAGCTTCACCATGGGCCATGTAT TTGATAGCCTGTGCACTGTGGACCAGAGGCCAGAAAGTTATAACCCCAATGATGTCATCACTTTTGAAGG AACTGGGGACCCATCTCTGTGTAAGGAGAAGGTGGCTTCCATATTTGACTTCAAAGCTTGCCATGATCAA GAAACCTGTTCTTTTGATGGGGTTTATCAGCCAAAGATTAAAGGGCCATTTGTGGCTTTTGCAGGATTCT ACTACACAGCCAGTGCTTTAAATCTTTCAGGTAGCTTTTCCCTGGACACCTTCAACTCCAGCACCTGGAA TTTCTGCTCACAGAATTGGAGTCAGCTCCCACTGCTGCTCCCCAAATTTGATGAGGTATATGCCCGCTCT TACTGCTTCTCAGCCAACTACATCTACCACTTGTTTGTGAACGGTTACAAATTCACAGAGGAGACTTGGC CCCAAATACACTTTGAAAAAGAAGTGGGGAATAGCAGCATAGCCTGGTCTCTTGGCTACATGCTCAGCCT GACCAACCAGATCCCAGCTGAAAGCCCTCTGATCCGTCTGCCCATAGAACCACCTGTCTTTGTGGGCACC CTCGCTTTCTTCACAGCGGCAGCCTTGCTGTGTCTGGCATTTCTTGCATACCTGTGTTCAGCAACCAGAA GAAAGAGGCACTCCGAGCATGCCTTTGACCATGCAGTGGATTCTGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238734 representing NM_001291960 Red=Cloning site Green=Tags(s) MFTVLTRQPCEQAGLKALYRTPTIIALVVLLVSIVVLVSITVIQIHKQEVLPPGLKYGIVLDAGSSRTTV YVYQWPAEKENNTGVVSQTFKCSVKGSGISSYGNNPQDVPRAFEECMQKVKGQVPSHLHGSTPIHLGATA GMRLLRLQNETAANEVLESIQSYFKSQPFDFRGAQIISGQEEGVYGWITANYLMGNFLEKNLWHMWVHPH GVETTGALDLGGASTQISFVAGEKMDLNTSDIMQVSLYGYVYTLYTHSFQCYGRNEAEKKFLAMLLQNSP TKNHLTNPCYPRDYSISFTMGHVFDSLCTVDQRPESYNPNDVITFEGTGDPSLCKEKVASIFDFKACHDQ ETCSFDGVYQPKIKGPFVAFAGFYYTASALNLSGSFSLDTFNSSTWNFCSQNWSQLPLLLPKFDEVYARS YCFSANYIYHLFVNGYKFTEETWPQIHFEKEVGNSSIAWSLGYMLSLTNQIPAESPLIRLPIEPPVFVGT LAFFTAAALLCLAFLAYLCSATRRKRHSEHAFDHAVDSD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291960
ORF Size	1587 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291960.2
RefSeq Size	2867 bp
RefSeq ORF	1590 bp
Locus ID	956
UniProt ID	O75355
Protein Families	Transmembrane
Protein Pathways	Purine metabolism, Pyrimidine metabolism
MW	59.6 kDa
Gene Summary	This gene encodes a plasma membrane-bound divalent cation-dependent E-type nucleotidase. The encoded protein is involved in the regulation of extracellular levels of ATP by hydrolysis of it and other nucleotides. Multiple transcript variants have been described. [provided by RefSeq, May 2014]

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