2 Hydroxy phytanoyl CoA lyase (HACL1) (NM_001284416) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2-HPCL; HPCL; HPCL2; PHYH2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238566 representing NM_001284416 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGACAGTAACTTCGCAGAGCGCAGCGAGGAGCAGGTGTCTGGTGCTAAAGTCATCGCTCAGGCCC TGAAAACGCAAGATGTGGAGTACATATTTGGCATCGTAGGCATCCCAGTGACCGAAATCGCCATTGCTGC CCAGCAGCTAGGCATCAAGTACATCGGGATGAGGAATGAGCAAGCGGCTTGTTATGCTGCCTCCGCGATT GGATATCTGACAAGCAGGCCAGGAGTCTGCCTTGTTGTTTCTGGCCCAGGTCTCATCCATGCCTTGGGCG GTATGGCAAATGCAAACATGAACTGCTGGTACATGGAACGCTGCATGTCACCTCCTATTAGCATGGCAGA AACCTCTGCTGTGTGCACGGCGGCTTCTGTTATTAGGAATGCCAAACAACCCCTTCTTATCATCGGGAAA GGTGCTGCTTACGCTCATGCAGAAGAGAGTATCAAGAAATTGGTGGAGCAATATAAACTGCCATTTTTGC CCACCCCTATGGGGAAGGGTGTTGTCCCTGACAACCATCCATACTGTGTAGGTGCAGCCAGATCCAGGGC TTTGCAATTTGCTGATGTAATTGTGTTATTTGGTGCCAGACTAAATTGGATTTTACATTTTGGACTGCCT CCAAGATATCAGCCAGATGTGAAGTTTATCCAGGTTGATATCTGTGCAGAAGAATTGGGGAATAATGTAA AGCCCGCTGTTACTTTGCTAGGAAACATACATGCTGTCACTAAGCAGCTTTTAGAGGAACTTGATAAAAC ACCATGGCAGTATCCTCCAGAGAGCAAGTGGTGGAAAACTCTGAGAGAAAAAATGAAGAGCAATGAAGCT GCATCCAAGGAACTAGCTTCTAAAAAATCCCTGCCTATGAATTATTACACAGTATTCTACCATGTTCAAG AACAACTACCTAGAGACTGTTTCGTGGTAAGTGAAGGAGCAAATACTATGGACATTGGACGGACTGTGCT TCAGAACTACCTTCCTCGTCACAGGCTTGATGCTGGTACTTTCGGAACAATGGGAGTTGGTTTGGGATTT GCTATTGCAGCTGCCGTGGTGGCTAAAGATAGAAGCCCTGGGCAATGGATCATCTGTGTGGAAGGAGACA GTGCATTTGGGTTTTCTGGCATGGAGGTAGAAACCATCTGCAGGTACAACTTGCCAATCATACTGTTGGT AGTGAATAACAATGGAATTTACCAAGGTTTTGATACAGATACTTGGAAAGAAATGTTAAAATTTCAAGAT GCTACTGCAGTGGTCCCTCCAATGTGTTTGCTGCCAAATTCACATTATGAGCAAGTCATGACTGCATTTG GAGGCAAAGGGTATTTTGTACAAACACCAGAAGAACTCCAAAAATCCCTGAGGCAGAGCCTAGCAGACAC AACTAAACCTTCTCTTATCAACATCATGATTGAGCCACAAGCCACACGGAAGGCCCAGGATTTTCATTGG CTGACCCGCTCTAATATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238566 representing NM_001284416 Red=Cloning site Green=Tags(s) MPDSNFAERSEEQVSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQLGIKYIGMRNEQAACYAASAI GYLTSRPGVCLVVSGPGLIHALGGMANANMNCWYMERCMSPPISMAETSAVCTAASVIRNAKQPLLIIGK GAAYAHAEESIKKLVEQYKLPFLPTPMGKGVVPDNHPYCVGAARSRALQFADVIVLFGARLNWILHFGLP PRYQPDVKFIQVDICAEELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPPESKWWKTLREKMKSNEA ASKELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMDIGRTVLQNYLPRHRLDAGTFGTMGVGLGF AIAAAVVAKDRSPGQWIICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKFQD ATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLRQSLADTTKPSLINIMIEPQATRKAQDFHW LTRSNM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284416
ORF Size	1488 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284416.2
RefSeq Size	2041 bp
RefSeq ORF	1491 bp
Locus ID	26061
UniProt ID	Q9UJ83
MW	55.2 kDa
Gene Summary	Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.[UniProtKB/Swiss-Prot Function]

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