VPS45A (VPS45) (NM_001279355) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A; VSP45; VSP45A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238285 representing NM_001279355 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGTGGTTTTTGCTGTGAAGCAGTACCCACATTTGTTTTCCCTCAATATTTTGGGTTGCTGCCAGG GTCGAAATTGGGATCCAGCCCAGCTATCTAGAACAACTCAAGGGCTTACAGCTCTCCTTTTATCTCTGAA GAAGTGTCCCATGATTCGTTATCAGCTCTCATCAGAGGCAGCAAAGAGACTTGCAGAGTGCGTTAAGCAA GTGATAACTAAAGAATATGAACTGTTTGAATTCCGTCGGACAGAGGTTCCTCCATTGCTCCTTATTTTAG ATCGCTGTGATGATGCCATCACCCCATTGCTAAACCAGTGGACATATCAGGCCATGGTCCACGAACTACT AGGCATAAACAACAATCGGATTGATCTTTCCAGAGTGCCGGGAATCAGTAAAGACTTAAGAGAAGTGGTC CTATCTGCTGAAAATGATGAATTCTATGCTAATAATATGTACCTGAACTTTGCTGAGATTGGTAGCAATA TAAAGAATCTCATGGAAGATTTTCAGAAGAAGAAACCAAAAGAACAGCAAAAACTAGAATCAATAGCAGA CATGAAGGCGTTTGTTGAGAATTATCCACAGTTCAAGAAAATGTCTGGGACTGTTTCAAAGCATGTGACA GTGGTTGGAGAACTGTCTCGATTGGTCAGTGAACGGAATCTGCTGGAGGTTTCAGAGGTTGAGCAAGAAC TGGCCTGTCAAAATGACCATTCTAGTGCTCTCCAGAATATAAAAAGGCTTCTGCAGAACCCCAAAGTGAC AGAGTTTGATGCTGCCCGCCTGGTGATGCTTTATGCTTTACATTATGAGCGACACAGCAGCAATAGCCTG CCAGGACTAATGATGGACCTCAGGAATAAAGGTGTTTCTGAGAAGTATCGAAAGCTCGTGTCTGCAGTTG TTGAATATGGTGGTAAACGAGTCAGAGGAAGTGACCTCTTCAGCCCCAAAGATGCTGTGGCTATCACCAA ACAATTCCTCAAAGGACTGAAGGGAGTAGAAAATGTATATACACAGCATCAACCTTTCCTACATGAAACC CTGGATCATCTCATCAAAGGAAGGCTTAAGGAAAACCTATATCCTTATTTAGGCCCCAGCACACTCAGAG ACAGACCTCAGGATATCATTGTGTTTGTAATTGGAGGAGCCACCTATGAAGAGGCTCTAACAGTTTATAA CCTGAACCGCACCACTCCTGGAGTGAGGATTGTCCTGGGAGGCACCACAGTGCACAACACGAAAAGTTTC CTAGAGGAAGTTCTGGCTTCTGGACTGCACAGCCGAAGCAAGGAGAGCTCTCAAGTCACATCAAGGTCAG CGAGCAGAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238285 representing NM_001279355 Red=Cloning site Green=Tags(s) MNVVFAVKQYPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLAECVKQ VITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVV LSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVT VVGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSL PGLMMDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHET LDHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSF LEEVLASGLHSRSKESSQVTSRSASRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001279355
ORF Size	1341 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001279355.1, NP_001266284.1
RefSeq Size	2080 bp
RefSeq ORF	1344 bp
Locus ID	11311
Protein Pathways	Endocytosis
MW	51.2 kDa
Gene Summary	Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013]

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