BECN2 (NM_001290693) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BECN1L1; BECN1P1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238188 representing NM_001290693 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCCATCCGCTTCCTGTGCCAGCGCTGCCACCAGGCCCTGAAGCTGAGCGGCTCCTCGGAGTCTA GGAGCCTCCCTGCAGCCCCGGCGCCCACCTCTGGGCAGGCTGAGCCCGGAGACACCCGGGAGCCCGGCGT CACCACCAGGGAGGTGACAGACGCTGAGGAGCAACAGGACGGTGCCTCTAGCAGATCCCCTCCAGGCGAT GGCAGTGTGTCCAAGGGCCATGCCAACATCTTCACCCTGCTGGGGGAGCTTGGCGCCATGCACATGCTCA GTAGCATCCAGAAGGCAGCTGGTGACATTTTTGACATAGTCTCTGGCCAAGCAGTTGTGGACCATCCCCT GTGTGAAGAATGCACCGACAGTCTTTTAGAGCAGCTGGACATCCAGCTCGCTCTCACAGAAGCTGACAGT CAGAACTACCAACGCTGCCTGGAGACCGGGGAGCTGGCGACCAGCGAGGACGAGGCGGCGGCGCTGCGGG CGGAGCTGCGGGACCTGGAGCTGGAGGAGGCCAGGCTGGTGCAGGAGCTGGAGGATGTGGACAGGAACAA TGCAAGAGCAGCGGCGGATCTCCAGGCAGCCCAGGCAGAGGCTGCGGAGCTGGACCAGCAGGAGAGGCAG CACTACAGGGACTACAGTGCCTTGAAGCGGCAGCAGCTGGAACTGCTTGATCAGCTGGGGAACGTGGAGA ACCAGCTGCAGTATGCCAGGGTCCAGAGGGACCGGCTGAAGGAAATCAACTGTTTCACCGCCACGTTTGA GATCTGGGTGGAGGGCCCCTTGGGCGTCATCAATAACTTCAGGTTGGGCCGCCTCCCCACTGTCCGTGTG GGCTGGAATGAGATTAACACTGCCTGGGGACAGGCGGCCTTGCTGCTCCTTACCCTGGCCAATACAATTG GACTGCAGTTTCAGAGGTATCGACTCATCCCCTGCGGAAACCATTCGTATCTGAAGTCTTTAACAGATGA CCGCACTGAGCTGCCGTTGTTCTGTTATGGGGGGCAGGATGTTTTCCTCAATAACAAGTATGACCGCGCG ATGGTGGCCTTCCTGGACTGCATGCAGCAGTTCAAGGAAGAGGCTGAGAAGGGTGAGCTGGGCCTCTCTC TGCCCTACGGGATCCAGGTGGAGACAGGCCTGATGGAGGACGTTGGCGGCCGAGGGGAATGCTATTCCAT CAGAACCCATCTGAACACGCAGGAGCTGTGGACAAAGGCACTCAAGTTCATGCTTATAAATTTCAAGTGG AGTCTCATCTGGGTTGCCTCAAGGTATCAAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238188 representing NM_001290693 Red=Cloning site Green=Tags(s) MSSIRFLCQRCHQALKLSGSSESRSLPAAPAPTSGQAEPGDTREPGVTTREVTDAEEQQDGASSRSPPGD GSVSKGHANIFTLLGELGAMHMLSSIQKAAGDIFDIVSGQAVVDHPLCEECTDSLLEQLDIQLALTEADS QNYQRCLETGELATSEDEAAALRAELRDLELEEARLVQELEDVDRNNARAAADLQAAQAEAAELDQQERQ HYRDYSALKRQQLELLDQLGNVENQLQYARVQRDRLKEINCFTATFEIWVEGPLGVINNFRLGRLPTVRV GWNEINTAWGQAALLLLTLANTIGLQFQRYRLIPCGNHSYLKSLTDDRTELPLFCYGGQDVFLNNKYDRA MVAFLDCMQQFKEEAEKGELGLSLPYGIQVETGLMEDVGGRGECYSIRTHLNTQELWTKALKFMLINFKW SLIWVASRYQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290693
ORF Size	1293 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290693.1, NP_001277622.1
RefSeq Size	1296 bp
RefSeq ORF	1296 bp
Locus ID	441925
UniProt ID	A8MW95
Protein Pathways	Regulation of autophagy
MW	48.2 kDa
Gene Summary	Involved in 2 distinct lysosomal degradation pathways: acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. Regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1.[UniProtKB/Swiss-Prot Function]

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