ACADS (NM_001302554) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ACAD3; SCAD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238026 representing NM_001302554 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGCGCTGCTCGCCCGGGCCTCGGGCCCTGCCCGCAGAGCTCTCTGTCCTAGGGCCTGGCGGC AGTTACACACCATCTACCAGTCTGTGGAACTGCCCGAGACACACCAGATGTTGCTCCAGACATGCCGGGA CTTTGCCGAGAAGGAGTTGTTTCCCATTGCAGCCCAGGTGGATAAGGAACATCTCTTCCCAGCGGCTCAG GTGAAGAAGATGGGCGGGCTTGGGCTTCTGGCCATGGACGTGCCCGAGGAGCTTGGCGGTGCTGGCCTCG ATTACCTGGCCTACGCCATCGCCATGGAGGAGATCAGCCGTGGCTGCGCCTCCACCGGAGTCATCATGAG TGTCAACAACTCTCTCTACCTGGGGCCCATCTTGAAGTTTGGCTCCAAGGAGCAGAAGCAGGCGTGGGTC ACGCCTTTCACCAGTGGTGACAAAATTGGCTGCTTTGCCCTCAGCGAACCAGGGCCTTCTCTCCTTGGCC CGACTGGACCTATTTTTGCTCTGGGGCAAGTGGGCTGTCCCTGTCCCTCCTCAGCTGCCACTGAAGCCTG CACCTTCCCCAGAAGCCGGCAGAGGGTGTCAAGGCCTGAGCTTCTGAGGGAGGGCATCAGTGCCTTCCTG GTCCCCATGCCAACGCCTGGGCTCACGTTGGGGAAGAAAGAAGACAAGCTGGGCATCCGGGGCTCATCCA CGGCCAACCTCATCTTTGAGGACTGTCGCATCCCCAAGGACAGCATCCTGGGGGAGCCAGGGATGGGCTT CAAGATAGCCATGCAAACCCTGGACATGGGCCGCATCGGCATCGCCTCCCAGGCCCTGGGCATTGCCCAG ACCGCCCTCGATTGTGCTGTGAACTACGCTGAGAATCGCATGGCCTTCGGGGCGCCCCTCACCAAGCTCC AGGTCATCCAGTTCAAGTTGGCAGACATGGCCCTGGCCCTGGAGAGTGCCCGGCTGCTGACCTGGCGCGC TGCCATGCTGAAGGATAACAAGAAGCCTTTCATCAAGGAGGCAGCCATGGCCAAGCTGGCCGCCTCGGAG GCCGCGACCGCCATCAGCCACCAGGCCATCCAGATCCTGGGCGGCATGGGCTACGTGACAGAGATGCCGG CAGAGCGGCACTACCGCGACGCCCGCATCACTGAGATCTACGAGGGCACCAGCGAAATCCAGCGGCTGGT GATCGCCGGGCATCTGCTCAGGAGCTACCGGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238026 representing NM_001302554 Red=Cloning site Green=Tags(s) MAAALLARASGPARRALCPRAWRQLHTIYQSVELPETHQMLLQTCRDFAEKELFPIAAQVDKEHLFPAAQ VKKMGGLGLLAMDVPEELGGAGLDYLAYAIAMEEISRGCASTGVIMSVNNSLYLGPILKFGSKEQKQAWV TPFTSGDKIGCFALSEPGPSLLGPTGPIFALGQVGCPCPSSAATEACTFPRSRQRVSRPELLREGISAFL VPMPTPGLTLGKKEDKLGIRGSSTANLIFEDCRIPKDSILGEPGMGFKIAMQTLDMGRIGIASQALGIAQ TALDCAVNYAENRMAFGAPLTKLQVIQFKLADMALALESARLLTWRAAMLKDNKKPFIKEAAMAKLAASE AATAISHQAIQILGGMGYVTEMPAERHYRDARITEIYEGTSEIQRLVIAGHLLRSYRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302554
ORF Size	1224 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302554.2
RefSeq Size	1952 bp
RefSeq ORF	1227 bp
Locus ID	35
UniProt ID	P16219
Protein Families	Druggable Genome
Protein Pathways	Butanoate metabolism, Fatty acid metabolism, Metabolic pathways, Valine, leucine and isoleucine degradation
MW	44.5 kDa
Gene Summary	This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014]

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