UTY (NM_001258265) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KDM6AL; KDM6C; UTY1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235247 representing NM_001258265 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCCTGCGCAGTGTCGCTCACTACCGCCGCTGTTGCCTTCGGTGATGAGGCAAAGAAAATGGCGG AAGGAAAAGCGAGCCGCGAGAGTGAAGAGGAGTCTGTTAGCCTGACAGTCGAGGAAAGGGAGGCGCTTGG TGGCATGGACAGCCGTCTCTTCGGGTTCGTGAGGCTTCATGAAGATGGCGCCAGAACGAAGACCCTACTA GGCAAGAATGCTGCGTTTTTATATGGCCTTGGTTTGGTCTACTTCTACTACAATGCATTTCATTGGGCAA TTAAAGCATTTCAAGATGTCCTTTATGTTGACCCCAGCTTTTGTCGAGCCAAGGAAATTCATTTACGACT TGGGCTCATGTTCAAAGTGAACACAGACTACAAGTCTAGTTTAAAGAGGAAGTATCATTCTGCAAAGGAG GCATATGAACAACTTTTGCAGACAGAAAACCTTCCTGCACAAGTAAAAGCAACTGTATTGCAACAGTTAG GTTGGATGCATCATAATATGGATCTAGTAGGAGACAAAGCCACAAAGGAAAGCTATGCTATTCAGTATCT CCAAAAGTCTTTGGAGGCAGATCCTAATTCTGGCCAATCGTGGTATTTTCTTGGAAGGTGTTATTCAAGT ATTGGGAAAGTTCAGGATGCCTTTATATCTTACAGGCAATCTATTGATAAATCAGAAGCAAGTGCAGATA CATGGTGTTCAATAGGTGTGTTGTATCAGCAGCAAAATCAGCCTATGGATGCTTTACAGGCATATATTTG TGCTGTACAATTGGACCATGGGCATGCCGCAGCCTGGATGGACCTAGGTACTCTCTATGAATCCTGCAAT CAACCTCAAGATGCCATTAAATGCTACCTAAATGCAGCTAGAAGCAAACGTTGTAGTAATACCTCTACGC TTGCTGCAAGAATTAAATTTCTACAGAATGGTTCTGATAACTGGAATGGTGGCCAGAGTCTTTCACATCA TCCAGTACAGCAAGTTTATTCGTTGTGTTTGACACCACAGAAATTACAGCACTTGGAACAACTGCGAGCA AATAGAGATAATTTAAATCCAGCACAGAAGCATCAGCTGGAACAGTTAGAAAGTCAGTTTGTCTTAATGC AGCAAATGAGACACAAAGAAGTTGCTCAGGTACGAACTACTGGAATTCATAACGGGGCCATAACTGATTC ATCACTGCCTACAAACTCTGTCTCTAATCGACAACCACATGGTGCTCTGACCAGAGTATCTAGCGTCTCT CAGCCTGGAGTTCGCCCTGCTTGTGTTGAAAAACTTTTGTCCAGTGGAGCTTTTTCTGCAGGCTGTATTC CTTGTGGCACATCAAAAATTCTAGGAAGTACAGACACTATCTTGCTAGGCAGTAATTGTATAGCAGGAAG TGAAAGTAATGGAAATGTGCCTTACCTGCAGCAAAATACACACACTCTACCTCATAATCATACAGACCTG AACAGCAGCACAGAAGAGCCATGGAGAAAACAGCTATCTAACTCCGCTCAGGGGCTTCATAAAAGTCAGA GTTCATGTTTGTCAGGACCTAATGAAGAACAACCTCTGTTTTCCACTGGGTCAGCCCAGTATCACCAGGC AACTAGCACTGGTATTAAGAAGGCGAATGAACATCTCACTCTGCCTAGTAATTCAGTACCACAGGGGGAT GCTGACAGTCACCTCTCCTGTCATACTGCTACCTCAGGTGGACAACAAGGCATTATGTTTACCAAAGAGA GCAAGCCTTCAAAAAATAGATCCTTGGTGCCTGAAACAAGCAGGCATACTGGAGACACATCTAATGGCTG TGCTGATGTCAAGGGACTTTCTAATCATGTTCATCAGTTGATAGCAGATGCTGTTTCCAGTCCTAACCAT GGAGATTCACCAAATTTATTAATTGCAGACAATCCTCAGCTCTCTGCTTTGTTGATTGGAAAAGCCAATG GCAATGTGGGTACTGGAACCTGTGACAAAGTGAATAATATTCACCCAGCTGTTCATACAAAGACTGATCA TTCTGTTGCCTCTTCACCCTCTTCAGCCATTTCCACAGCAACACCTTCTCCTAAATCCACTGAGCAGAGA AGCATAAACAGTGTTACCAGCCTTAACAGTCCTCACAGTGGATTACACACAGTCAATGGAGAGGGGCTGG GGAAGTCACAGAGCTCTACAAAAGTAGACCTGCCTTTAGCTAGCCACAGATCTACTTCTCAGATCTTACC ATCAATGTCAGTGTCTATATGCCCCAGTTCAACAGAAGTTCTGAAAGCATGCAGGAATCCAGGTAAAAAT GGCTTGTCTAATAGCTGCATTTTGTTAGATAAATGTCCACCTCCAAGACCACCAACTTCACCATACCCAC CCTTGCCAAAGGACAAGTTGAATCCACCCACACCTAGTATTTACTTGGAAAATAAACGTGATGCTTTCTT TCCTCCATTACATCAATTTTGTACAAATCCAAAAAACCCTGTTACAGTAATACGTGGCCTTGCTGGAGCT CTTAAATTAGATCTTGGACTTTTCTCTACCAAAACTTTGGTAGAAGCTAACAATGAACATATGGTAGAAG TGAGGACACAGTTGCTGCAACCAGCAGATGAAAACTGGGATCCCACTGGAACAAAGAAAATCTGGCGTTG TGAAAGCAATAGATCTCATACTACAATTGCCAAATACGCACAATACCAGGCTTCCTCCTTCCAGGAATCA TTGAGAGAAGAAAATGAGAAAAGAACACAACACAAAGATCATTCAGATAACGAATCCACATCTTCAGAGA ATTCTGGAAGGAGAAGGAAAGGACCTTTTAAAACCATAAAATTTGGGACCAACATTGACCTCTCTGATAA CAAAAAGTGGAAGTTGCAGTTACATGAACTGACTAAACTTCCTGCTTTTGCGCGTGTGGTGTCAGCAGGA AATCTTCTAACCCATGTTGGGCATACCATTCTGGGCATGAATACAGTACAACTGTATATGAAAGTTCCAG GGAGTCGGACACCAGGTCACCAAGAAAATAACAACTTCTGCTCTGTTAACATAAATATTGGTCCAGGAGA TTGTGAATGGTTTGTTGTACCTGAAGATTATTGGGGTGTTCTGAATGACTTCTGTGAAAAAAATAATTTG AATTTTTTAATGAGTTCTTGGTGGCCCAACCTTGAAGATCTTTATGAAGCAAATGTCCCTGTGTATAGAT TTATTCAGCGACCTGGAGATTTGGTCTGGATAAATGCAGGCACTGTGCATTGGGTTCAAGCTGTTGGCTG GTGCAATAACATTGCCTGGAATGTTGGTCCACTTACAGCCTGCCAGTATAAATTGGCAGTGGAACGGTAT GAATGGAACAAATTGAAAAGTGTGAAGTCACCAGTACCCATGGTGCATCTTTCCTGGAATATGGCACGAA ATATCAAAGTCTCAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTTTGAAAATTCTGAAGCAATA TCAGACATTGAGAGAAGCTCTTGTTGCAGCAGGAAAAGAGGTTATATGGCATGGGCGGACAAATGATGAA CCAGCTCATTACTGTAGCATTTGTGAGGTGGAGGTTTTTAATCTGCTTTTTGTCACTAATGAAAGCAATA CTCAAAAAACCTACATAGTACATTGCCATGATTGTGCACGAAAAACAAGCAAAAGTTTGGAAAATTTTGT GGTGCTCGAACAGTACAAAATGGAGGACCTAATCCAAGTTTATGATCAATTTACACTAGCTCTTTCATTA TCATCCTCATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235247 representing NM_001258265 Red=Cloning site Green=Tags(s) MKSCAVSLTTAAVAFGDEAKKMAEGKASRESEEESVSLTVEEREALGGMDSRLFGFVRLHEDGARTKTLL GKNAAFLYGLGLVYFYYNAFHWAIKAFQDVLYVDPSFCRAKEIHLRLGLMFKVNTDYKSSLKRKYHSAKE AYEQLLQTENLPAQVKATVLQQLGWMHHNMDLVGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSS IGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCN QPQDAIKCYLNAARSKRCSNTSTLAARIKFLQNGSDNWNGGQSLSHHPVQQVYSLCLTPQKLQHLEQLRA NRDNLNPAQKHQLEQLESQFVLMQQMRHKEVAQVRTTGIHNGAITDSSLPTNSVSNRQPHGALTRVSSVS QPGVRPACVEKLLSSGAFSAGCIPCGTSKILGSTDTILLGSNCIAGSESNGNVPYLQQNTHTLPHNHTDL NSSTEEPWRKQLSNSAQGLHKSQSSCLSGPNEEQPLFSTGSAQYHQATSTGIKKANEHLTLPSNSVPQGD ADSHLSCHTATSGGQQGIMFTKESKPSKNRSLVPETSRHTGDTSNGCADVKGLSNHVHQLIADAVSSPNH GDSPNLLIADNPQLSALLIGKANGNVGTGTCDKVNNIHPAVHTKTDHSVASSPSSAISTATPSPKSTEQR SINSVTSLNSPHSGLHTVNGEGLGKSQSSTKVDLPLASHRSTSQILPSMSVSICPSSTEVLKACRNPGKN GLSNSCILLDKCPPPRPPTSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPKNPVTVIRGLAGA LKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWRCESNRSHTTIAKYAQYQASSFQES LREENEKRTQHKDHSDNESTSSENSGRRRKGPFKTIKFGTNIDLSDNKKWKLQLHELTKLPAFARVVSAG NLLTHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEDYWGVLNDFCEKNNL NFLMSSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPLTACQYKLAVERY EWNKLKSVKSPVPMVHLSWNMARNIKVSDPKLFEMIKYCLLKILKQYQTLREALVAAGKEVIWHGRTNDE PAHYCSICEVEVFNLLFVTNESNTQKTYIVHCHDCARKTSKSLENFVVLEQYKMEDLIQVYDQFTLALSL SSSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258265
ORF Size	3792 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258265.1, NP_001245194.1
RefSeq Size	6277 bp
RefSeq ORF	3795 bp
Locus ID	7404
Protein Families	Transmembrane
MW	140.3 kDa
Gene Summary	This gene encodes a protein containing tetratricopeptide repeats which are thought to be involved in protein-protein interactions. The encoded protein is also a minor histocompatibility antigen which may induce graft rejection of male stem cell grafts. A large number of alternatively spliced transcripts have been observed for this gene, but the full length nature of some of these variants has not been determined. [provided by RefSeq, Apr 2012]

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