RAD54B (NM_001205263) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RDH54
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234779 representing NM_001205263 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTTGTGGAAAAGAAATAGAAGTCATGGGTGTAATCTCTCCAGATGACTTCAGCAGTGGCAGGTGTT TTCAGCTTGGAGGAGGAAGTACTGCTATCTCGCATTCTTCTCAGGTTGCCAGGAAATGTTTCTCTAACCC TTTCAAAAGTGTTTGTAAACCAAGTTCAAAGGAAAATAGACAGAATGATTTCCAAAATTGCAAACCACGC CATGACCCATATACGCCAAATTCCCTCGTTATGCCACGACCAGATAAGAATCACCAGTGGGTATTCAATA AGAACTGTTTCCCTCTTGTGGATGTAGTGATTGATCCTTACCTTGTATATCATCTTCGACCACATCAGAA AGAAGGAATCATATTCCTTTATGAATGTGTAATGGGAATGAGAATGAATGGCAGATGTGGAGCTATTCTT GCTGATGAAATGGGTTTAGGGAAGACATTGCAATGTATTTCGCTCATCTGGACCCTGCAGTGTCAGGGAC CCTATGGAGGCAAGCCAGTAATAAAGAAGACACTAATTGTCACACCTGGAAGCTTGGTGAATAATTGGAA GAAAGAATTTCAAAAATGGCTAGGAAGTGAAAGGATCAAGATATTTACTGTTGATCAGGACCACAAAGTT GAAGAATTCATCAAGTCTATATTTTATTCTGTTCTTATTATCAGTTATGAAATGTTACTTCGTTCCCTGG ATCAAATTAAGAATATAAAATTTGATCTTCTAATCTGTGACGAGGGGCATCGTTTGAAGAACAGTGCCAT TAAGACAACTACAGCCCTCATTAGCCTTTCTTGTGAGAAAAGAATAATTCTAACTGGTACTCCAATTCAG AATGATCTGCAAGAATTTTTTGCATTAATTGATTTTGTAAATCCAGGAATATTAGGCTCTTTGTCATCTT ATAGGAAAATATATGAAGAACCCATCATTTTATCGAGAGAACCTTCTGCTTCTGAGGAAGAAAAGGAGTT AGGAGAAAGAAGAGCAGCTGAACTTACTTGCCTCACTGGACTCTTTATCCTTAGAAGAACCCAAGAAATT ATAAATAAATATCTCCCACCTAAAATAGAGAATGTTGTCTTTTGCCGACCAGGAGCACTACAGATTGAGC TTTATCGAAAGCTGTTAAATTCTCAGGTTGTCAGGTTCTGCCTTCAAGGGTTGTTGGAAAATAGTCCCCA TCTAATATGTATAGGAGCTCTTAAAAAACTGTGCAATCACCCCTGCCTTTTGTTCAACTCTATAAAGGAA AAGGAATGTAGCTCAACTTGTGATAAAAATGAAGAAAAGAGTCTATACAAAGGCTTGCTAAGTGTGTTTC CTGCTGACTACAACCCTCTCCTGTTTACTGAAAAGGAGTCAGGAAAACTACAGGTGTTGTCCAAGCTCTT AGCGGTTATCCACGAACTTCGACCTACTGAAAAGGTGGTGTTGGTATCCAACTATACACAAACCTTGAAC ATTTTACAAGAAGTATGTAAGCGTCATGGATATGCTTATACAAGACTTGATGGACAAACACCAATCTCTC AAAGGCAGCAGATTGTTGATGGCTTTAACAGTCAACACTCTTCTTTTTTTATTTTTTTGTTAAGTTCAAA AGCTGGTGGTGTAGGACTTAACCTCATTGGAGGATCTCACTTAATTCTCTATGACATTGATTGGAATCCA GCCACTGACATTCAGGCAATGTCTAGAGTATGGAGAGATGGTCAGAAATATCCTGTACATATTTACAGAC TCCTAACTACAGGTACAATAGAAGAAAAGATCTATCAAAGGCAGATCAGTAAGCAAGGTCTTTGTGGGGC AGTTGTCGACCTCACCAAGACATCTGAACATATTCAGTTTTCAGTAGAAGAACTTAAAAATTTGTTCACA TTACATGAAAGTTCAGATTGTGTTACTCATGATCTGCTTGACTGTGAGTGTACAGGAGAAGAAGTTCATA CAGGTGATTCGTTGGAAAAATTCATTGTCTCTAGAGATTGTCAGCTTGGTCCACATCACCAGAAATCTAA CTCCCTGAAACCTCTTTCTATGTCCCAGCTGAAGCAATGGAAACATTTTTCTGGAGATCATTTAAATCTT ACAGATCCTTTTCTTGAAAGAATAACAGAAAATGTGTCATTCATTTTTCAGAATATAACCACTCAAGCTA CTGGCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234779 representing NM_001205263 Red=Cloning site Green=Tags(s) MICGKEIEVMGVISPDDFSSGRCFQLGGGSTAISHSSQVARKCFSNPFKSVCKPSSKENRQNDFQNCKPR HDPYTPNSLVMPRPDKNHQWVFNKNCFPLVDVVIDPYLVYHLRPHQKEGIIFLYECVMGMRMNGRCGAIL ADEMGLGKTLQCISLIWTLQCQGPYGGKPVIKKTLIVTPGSLVNNWKKEFQKWLGSERIKIFTVDQDHKV EEFIKSIFYSVLIISYEMLLRSLDQIKNIKFDLLICDEGHRLKNSAIKTTTALISLSCEKRIILTGTPIQ NDLQEFFALIDFVNPGILGSLSSYRKIYEEPIILSREPSASEEEKELGERRAAELTCLTGLFILRRTQEI INKYLPPKIENVVFCRPGALQIELYRKLLNSQVVRFCLQGLLENSPHLICIGALKKLCNHPCLLFNSIKE KECSSTCDKNEEKSLYKGLLSVFPADYNPLLFTEKESGKLQVLSKLLAVIHELRPTEKVVLVSNYTQTLN ILQEVCKRHGYAYTRLDGQTPISQRQQIVDGFNSQHSSFFIFLLSSKAGGVGLNLIGGSHLILYDIDWNP ATDIQAMSRVWRDGQKYPVHIYRLLTTGTIEEKIYQRQISKQGLCGAVVDLTKTSEHIQFSVEELKNLFT LHESSDCVTHDLLDCECTGEEVHTGDSLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNL TDPFLERITENVSFIFQNITTQATGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205263
ORF Size	2178 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205263.2
RefSeq Size	3097 bp
RefSeq ORF	2181 bp
Locus ID	25788
UniProt ID	Q9Y620
Protein Families	Druggable Genome
Protein Pathways	Homologous recombination
MW	82.8 kDa
Gene Summary	The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008]

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