ACSL6 (NM_001205247) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ACS2; FACL6; LACS2; LACS5; LACS 6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234715 representing NM_001205247 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGACCTTCTTCCTCGTGTCGGGGGGCTCCCTCTGGCTATTCGTAGAGTTTGTCCTCTCACTTCTGG AGAAGATGCAGACACAGGAGATCCTGAGGATACTGCGACTGCCTGAGCTAGGTGACTTGGGACAGTTTTT CCGCAGCCTCTCGGCCACCACCCTCGACAGTGGCGGGGCACGGCGATCTGTGATTGGGTCTGGCCCTCAG CTACTTACCCACTACTATGATGATGCCCGGACCATGTACCAGGTGTTCCGCCGTGGGCTTAGCATCTCAG GGAATGGGCCCTGTCTTGGTTTCAGGAAGCCTAAGCAGCCTTACCAGTGGCTGTCCTACCAGGAGGTGGC CGACAGGGCTGAATTTCTGGGGTCCGGACTTCTCCAGCACAATTGTAAAGCATGCACTGATCAGTTTATT GGTGTTTTTGCACAAAATCGGCCAGAGTGGATCATTGTGGAGCTGGCCTGCTACACATATTCCATGGTGG TGGTCCCGCTCTATGACACCCTGGGCCCTGGGGCTATCCGCTACATCATCAATACAGCGGACATCAGCAC CGTGATTGTGGACAAACCTCAGAAGGCTGTGCTTCTGCTAGAGCATGTGGAGAGGAAGGAGACTCCAGGC CTCAAGCTGATCATCCTCATGGACCCATTCGAAGAAGCCCTGAAAGAGAGAGGGCAGAAGTGCGGGGTGG TCATTAAGTCCATGCAGGCCGTGGAGGACTGTGGCCAAGAGAATCACCAGGCTCCTGTGCCCCCGCAGCC TGATGACCTCTCCATTGTGTGTTTCACAAGCGGCACGACAGGGAACCCAAAAGGTGCGATGCTCACCCAT GGGAACGTGGTGGCTGATTTCTCAGGCTTTCTGAAAGTGACAGAGAGTCAGTGGGCTCCCACTTGTGCGG ATGTGCACATTTCCTATTTGCCTTTAGCACACATGTTTGAGCGAATGGTGCAGTCTGTCGTCTATTGCCA CGGAGGGCGTGTTGGCTTCTTCCAGGGAGATATCCGCCTTCTCTCAGATGACATGAAGGCTCTATGCCCC ACCATCTTCCCTGTGGTCCCACGACTGCTGAACCGGATGTACGACAAGATCTTCAGCCAGGCAAACACAC CATTAAAGCGCTGGCTCCTGGAGTTTGCAGCAAAGCGTAAGCAAGCCGAGGTCCGGAGTGGAATCATCAG GAATGATAGTATCTGGGATGAACTCTTCTTTAATAAGATTCAGGCCAGTCTTGGTGGGTGTGTGCGGATG ATTGTTACTGGAGCAGCCCCAGCATCACCAACAGTTCTGGGATTTCTCCGGGCAGCTCTAGGGTGCCAGG TTTATGAAGGTTATGGCCAAACTGAGTGCACAGCTGGATGTACCTTCACCACTCCTGGCGACTGGACCTC AGGGCACGTAGGGGCGCCACTTCCCTGCAATCATATCAAGCTCGTTGATGTTGAGGAACTGAACTACTGG GCCTGCAAAGGAGAGGGAGAGATATGTGTGAGAGGACCAAATGTGTTCAAAGGCTACTTGAAAGATCCAG ACAGGACGAAGGAGGCCCTGGACAGCGATGGCTGGCTTCACACTGGAGACATCGGAAAATGGCTGCCGGC AGGAACTCTTAAAATTATTGATCGGAAAAAGCATATATTTAAACTTGCTCAGGGAGAATATGTTGCACCC GAGAAGATTGAGAACATCTACATCCGGAGCCAACCTGTGGCGCAAATCTATGTCCATGGGGACAGCTTAA AGGCCTTTTTGGTAGGCATTGTTGTGCCTGACCCTGAAGTTATGCCCTCCTGGGCCCAGAAGAGAGGAAT TGAAGGAACATATGCAGATCTCTGCACAAATAAGGATCTGAAGAAAGCCATTTTGGAAGATATGGTGAGG TTAGGAAAAGAAAGTGGACTCCATTCTTTTGAGCAGGTTAAAGCCATTCACATCCATTCTGACATGTTCT CAGTTCAAAATGGCTTGCTGACACCAACACTAAAAGCTAAGAGACCTGAGCTGAGAGAGTACTTCAAAAA ACAAATAGAAGAGCTTTACTCAATCTCCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234715 representing NM_001205247 Red=Cloning site Green=Tags(s) MLTFFLVSGGSLWLFVEFVLSLLEKMQTQEILRILRLPELGDLGQFFRSLSATTLDSGGARRSVIGSGPQ LLTHYYDDARTMYQVFRRGLSISGNGPCLGFRKPKQPYQWLSYQEVADRAEFLGSGLLQHNCKACTDQFI GVFAQNRPEWIIVELACYTYSMVVVPLYDTLGPGAIRYIINTADISTVIVDKPQKAVLLLEHVERKETPG LKLIILMDPFEEALKERGQKCGVVIKSMQAVEDCGQENHQAPVPPQPDDLSIVCFTSGTTGNPKGAMLTH GNVVADFSGFLKVTESQWAPTCADVHISYLPLAHMFERMVQSVVYCHGGRVGFFQGDIRLLSDDMKALCP TIFPVVPRLLNRMYDKIFSQANTPLKRWLLEFAAKRKQAEVRSGIIRNDSIWDELFFNKIQASLGGCVRM IVTGAAPASPTVLGFLRAALGCQVYEGYGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIKLVDVEELNYW ACKGEGEICVRGPNVFKGYLKDPDRTKEALDSDGWLHTGDIGKWLPAGTLKIIDRKKHIFKLAQGEYVAP EKIENIYIRSQPVAQIYVHGDSLKAFLVGIVVPDPEVMPSWAQKRGIEGTYADLCTNKDLKKAILEDMVR LGKESGLHSFEQVKAIHIHSDMFSVQNGLLTPTLKAKRPELREYFKKQIEELYSISM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205247
ORF Size	2061 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205247.2
RefSeq Size	6449 bp
RefSeq ORF	2064 bp
Locus ID	23305
UniProt ID	Q9UKU0
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
MW	77.1 kDa
Gene Summary	The protein encoded by this gene catalyzes the formation of acyl-CoA from fatty acids, ATP, and CoA, using magnesium as a cofactor. The encoded protein plays a major role in fatty acid metabolism in the brain. Translocations with the ETV6 gene are causes of myelodysplastic syndrome with basophilia, acute myelogenous leukemia with eosinophilia, and acute eosinophilic leukemia. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.