Histidase (HAL) (NM_001258334) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HIS; HSTD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234511 representing NM_001258334 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGATACACGGTGCACGTACGTGGGGAATGGCTGGCAGTGCCCTGCCAGGACGCGCAGCTCACTG TGGGCTGGCTGGGCCGGGAGGCCGTGAGGCGCTATATCAAGAATAAGCCCGACAATGGTGGCTTCACCTC CGTGGATGACGCGCACTTCCTTGTGCGCCGGTGCAAGGGCCTGGGCCTGCTGGACAACGAGGACCGGCTC GAGGTGGCCCTAGAGAACAACGAGTTCGTGGAAGTGGTTATAGAGGGTGATGCCATGTCTCCTGACTTCA TTCCATCTCAACCAGAAGGAGTTTATCTATACAGCAAGTACCGGGAGCCTGAAAAGTACATCGAGTTAGA TGGAGACCGTCTGACCACGGAGGATCTGGTCAACTTGGGAAAGGGACGCTACAAAATAAAGCTCACCCCA ACAGCTGAGAAGAGGGTGCAGAAATCCAGGGAGGTCATAGATAGCATCATAAAAGAGAAAACAGTTGTTT ACGGTATTACTACAGGTTTTGGGAAATTTGCCAGAACTGTAATTCCTATCAATAAGCTACAGGAGCTTCA GGTCAACTTAGTACGCTCACATTCTTCAGGTGTTGGGAAACCACTAAGTCCTGAGAGGTGTCGGATGCTC TTGGCTTTAAGGATCAATGTCTTAGCCAAAGGATACAGTGGCATTTCCCTGGAGACCCTCAAACAAGTCA TAGAAATGTTTAATGCCTCCTGCCTGCCCTATGTCCCAGAGAAAGGAACCGTTGGTGCCAGTGGAGACCT TGCCCCACTCTCTCATCTTGCTCTTGGGCTAGTTGGAGAAGGGAAGATGTGGTCTCCGAAGAGTGGCTGG GCTGATGCTAAATACGTGCTAGAAGCCCATGGATTGAAACCAGTTATTTTAAAACCAAAAGAGGGCCTGG CACTCATCAATGGGACGCAGATGATCACATCCCTGGGCTGTGAAGCTGTAGAGCGAGCCAGTGCTATTGC ACGGCAGGCTGACATTGTGGCAGCCCTGACCCTTGAGGTGCTGAAGGGCACCACCAAAGCCTTTGACACT GACATTCATGCTCTTCGACCTCACCGTGGGCAAATTGAAGTTGCTTTTCGGTTTCGGTCACTCTTGGACT CAGATCACCACCCATCAGAAATAGCAGAGAGTCACAGGTTCTGTGATCGCGTCCAGGATGCATACACCTT GCGCTGCTGTCCACAGGTCCATGGTGTGGTGAATGATACAATAGCATTTGTGAAGAACATCATTACCACA GAACTGAACAGCGCAACAGATAATCCTATGGTCTTTGCCAATAGGGGAGAGACAGTTTCTGGAGGAAACT TCCATGGTGAATACCCAGCCAAAGCCCTAGACTACTTGGCCATTGGCATCCATGAACTTGCTGCAATCAG TGAGAGAAGAATCGAGCGGCTCTGCAATCCCTCCCTCAGTGAGCTGCCTGCCTTCCTGGTGGCTGAAGGT GGTCTGAACTCTGGGTTCATGATAGCTCACTGCACGGCAGCAGCCCTTGTTTCTGAGAACAAGGCTCTGT GCCATCCCTCGTCTGTTGACTCCCTCTCCACCAGCGCAGCCACGGAGGACCACGTCTCCATGGGAGGATG GGCAGCAAGGAAAGCCCTCAGGGTCATCGAGCATGTGGAGCAAGTGCTGGCCATCGAGCTCCTTGCAGCC TGCCAGGGCATAGAGTTTCTACGTCCCCTGAAAACAACCACTCCGCTGGAGAAGGTCTATGACCTGGTGC GCTCTGTTGTAAGGTTTGGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234511 representing NM_001258334 Red=Cloning site Green=Tags(s) MPRYTVHVRGEWLAVPCQDAQLTVGWLGREAVRRYIKNKPDNGGFTSVDDAHFLVRRCKGLGLLDNEDRL EVALENNEFVEVVIEGDAMSPDFIPSQPEGVYLYSKYREPEKYIELDGDRLTTEDLVNLGKGRYKIKLTP TAEKRVQKSREVIDSIIKEKTVVYGITTGFGKFARTVIPINKLQELQVNLVRSHSSGVGKPLSPERCRML LALRINVLAKGYSGISLETLKQVIEMFNASCLPYVPEKGTVGASGDLAPLSHLALGLVGEGKMWSPKSGW ADAKYVLEAHGLKPVILKPKEGLALINGTQMITSLGCEAVERASAIARQADIVAALTLEVLKGTTKAFDT DIHALRPHRGQIEVAFRFRSLLDSDHHPSEIAESHRFCDRVQDAYTLRCCPQVHGVVNDTIAFVKNIITT ELNSATDNPMVFANRGETVSGGNFHGEYPAKALDYLAIGIHELAAISERRIERLCNPSLSELPAFLVAEG GLNSGFMIAHCTAAALVSENKALCHPSSVDSLSTSAATEDHVSMGGWAARKALRVIEHVEQVLAIELLAA CQGIEFLRPLKTTTPLEKVYDLVRSVVRFGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258334
ORF Size	1773 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258334.2
RefSeq Size	3857 bp
RefSeq ORF	1776 bp
Locus ID	3034
UniProt ID	P42357
Protein Families	Druggable Genome
Protein Pathways	Histidine metabolism, Metabolic pathways, Nitrogen metabolism
MW	65.3 kDa
Gene Summary	Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

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