Syntaxin 4 (STX4) (NM_001272095) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	p35-2; STX4A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233473 representing NM_001272095 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCAGGAGCTGCAGAACCTGCGCGATGAGATCAAACAGCTGGGGAGGGAGATCCGCCTGCAGCTGA AGGCCATAGAGCCCCAGAAGGAGGAAGCTGATGAGAACTATAACTCCGTCAACACAAGAATGAGAAAAAC CCAGCATGGGGTCCTGTCCCAGCAATTCGTGGAGCTCATCAACAAGTGCAATTCAATGCAGTCCGAATAC CGGGAGAAGAACGTGGAGCGGATTCGGAGGCAGCTGAAGATCACCAATGCTGGGATGGTGTCTGATGAGG AGTTGGAGCAGATGCTGGACAGTGGGCAAAGCGAGGTGTTTGTGTCCAATATCCTGAAGGACACGCAGGT GACTCGACAGGCCTTAAATGAGATCTCGGCCCGGCACAGTGAGATCCAGCAGCTTGAACGCAGTATTCGT GAGCTGCACGACATATTCACTTTTCTGGCTACCGAAGTGGAGATGCAGGGGGAGATGATCAATCGGATTG AGAAGAACATCCTGAGCTCAGCGGACTACGTGGAACGTGGGCAGGAGCACGTCAAGACGGCCCTGGAGAA CCAGAAGAAGGCGAGGAAGAAGAAAGTCTTGATTGCCATCTGTGTGTCCATCACCGTCGTCCTCCTAGCA GTCATCATTGGCGTCACAGTGGTTGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233473 representing NM_001272095 Red=Cloning site Green=Tags(s) MKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEY REKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIR ELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKKKVLIAICVSITVVLLA VIIGVTVVG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001272095
ORF Size	657 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001272095.1, NP_001259024.1
RefSeq Size	1630 bp
RefSeq ORF	660 bp
Locus ID	6810
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	SNARE interactions in vesicular transport
MW	25.7 kDa
Gene Summary	Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.[UniProtKB/Swiss-Prot Function]

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